DMRIE

    WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 465232

CAS#: 153312-64-2 (bromide)

Description: DMRIE is a cationic lipid for RNA and vaccine delivery. DMRIE is an important component for making DMRIE-C Transfection Reagent. DMRIE-C can also be used for in vivo delivery of DNA. DMRIE-C is a 1:1 (M/M) liposome formulation of the cationic lipid DMRIE (1,2-dimyristyloxy-propyl-3-dimethyl-hydroxy ethyl ammonium bromide) and cholesterol in membrane-filtered water.

Chemical Structure

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DMRIE
CAS# 153312-64-2 (bromide)
Instruction

Theoretical Analysis

MedKoo Cat#: 465232
Name: DMRIE
CAS#: 153312-64-2 (bromide)
Chemical Formula: C35H74BrNO3
Exact Mass: 635.49
Molecular Weight: 636.885
Elemental Analysis: C, 66.01; H, 11.71; Br, 12.55; N, 2.20; O, 7.54

Price and Availability

This product is not in stock, which may be available by custom synthesis. For cost-effective reason, minimum order is 1g (price is usually high, lead time is 2~3 months, depending on the technical challenge). Quote less than 1g will not be provided. To request quote, please email to sales @medkoo.com or click below button.
Note: Price will be listed if it is available in the future.

Request quote for custom synthesis

Related CAS #: 153312-64-2 (bromide)   191980-81-1 (cation),  

Synonym: DMRIE; dimyristyloxypropyl-3-dimethyl-hydroxyethyl ammonium;

IUPAC/Chemical Name: N-(2-hydroxyethyl)-N,N-dimethyl-2,3-bis(tetradecyloxy)propan-1-aminium bromide

InChi Key: WALUVDCNGPQPOD-UHFFFAOYSA-M

InChi Code: InChI=1S/C35H74NO3.BrH/c1-5-7-9-11-13-15-17-19-21-23-25-27-31-38-34-35(33-36(3,4)29-30-37)39-32-28-26-24-22-20-18-16-14-12-10-8-6-2;/h35,37H,5-34H2,1-4H3;1H/q+1;/p-1

SMILES Code: OCC[N+](C)(CC(COCCCCCCCCCCCCCC)OCCCCCCCCCCCCCC)C.[Br-]

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: To be determined

Shelf Life: >2 years if stored properly

Drug Formulation: To be determined

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Biological target:
In vitro activity:
In vivo activity:

Preparing Stock Solutions

The following data is based on the product molecular weight 636.89 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
In vitro protocol:
In vivo protocol:

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1: Kim BK, Doh KO, Hwang GB, Seu YB. Transfection property of a new cholesterol- based cationic lipid containing tri-2-hydroxyethylamine as gene delivery vehicle. J Microbiol Biotechnol. 2012 Jun;22(6):866-71. doi: 10.4014/jmb.1111.11010. PMID: 22573166.

2: Li XL, Lei Y, Zhong S, Peng FY, Zhou Z, Li K, Ren H. [Construction and functional characterization of a monocistronic replicon based on the HCV genotype 2a promotor]. Zhonghua Gan Zang Bing Za Zhi. 2012 Feb;20(2):103-7. Chinese. doi: 10.3760/cma.j.issn.1007-3418.2012.02.007. PMID: 22464780.

3: Lee SJ, Yoon SH, Doh KO. Enhancement of gene delivery using novel homodimeric tat peptide formed by disulfide bond. J Microbiol Biotechnol. 2011 Aug;21(8):802-7. doi: 10.4014/jmb.1105.05041. PMID: 21876369.

4: Goepfert C, Gazdhar A, Frey FJ, Frey BM. Effect of electroporation-mediated diphtheria toxin A expression on PSA positive human prostate xenograft tumors in SCID mice. Prostate. 2011 Jun 1;71(8):872-80. doi: 10.1002/pros.21303. Epub 2010 Nov 4. PMID: 21456069.

5: Wong AW, Baginski TK, Reilly DE. Enhancement of DNA uptake in FUT8-deleted CHO cells for transient production of afucosylated antibodies. Biotechnol Bioeng. 2010 Aug 1;106(5):751-63. doi: 10.1002/bit.22749. PMID: 20564613.

6: Branski LK, Masters OE, Herndon DN, Mittermayr R, Redl H, Traber DL, Cox RA, Kita K, Jeschke MG. Pre-clinical evaluation of liposomal gene transfer to improve dermal and epidermal regeneration. Gene Ther. 2010 Jun;17(6):770-8. doi: 10.1038/gt.2010.32. Epub 2010 Apr 8. PMID: 20376099.

7: Rhinn H, Largeau C, Bigey P, Kuen RL, Richard M, Scherman D, Escriou V. How to make siRNA lipoplexes efficient? Add a DNA cargo. Biochim Biophys Acta. 2009 Apr;1790(4):219-30. doi: 10.1016/j.bbagen.2009.01.005. PMID: 19714863.

8: Gong FY, Deng JY, Shi YF. Mek and p38 MAPK-dependant pathways are involoved in the positive effect of interleukin-6 on human growth hormone gene expression in rat MtT/S somatotroph cells. Chin Med Sci J. 2008 Jun;23(2):73-80. doi: 10.1016/s1001-9294(09)60016-x. PMID: 18686625.

9: Johler SM, Rejman J, Guan S, Rosenecker J. Nebulisation of IVT mRNA Complexes for Intrapulmonary Administration. PLoS One. 2015 Sep 9;10(9):e0137504. doi: 10.1371/journal.pone.0137504. PMID: 26352268; PMCID: PMC4564175.

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