MT1 | BET inhibitor | CAS#2060573-82-0

MT1 BET inhibitor
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WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 407972

CAS#: 2060573-82-0

Description: MT1, also known as (Bis-CPI203)-PEG7, is a highly potent BET bromodomain inhibitor. MT1 is an intramolecular bivalent BRD4 binder that is more than 100-fold more potent, in cellular assays, than the corresponding monovalent antagonist, JQ1. MT1 significantly (P < 0.05) delayed leukemia progression in mice, as compared to JQ1.

Chemical Structure

MT1 BET inhibitor

CAS# 2060573-82-0

Product data Instruction

Theoretical Analysis

MedKoo Cat#: 407972
Name: MT1 BET inhibitor
CAS#: 2060573-82-0
Chemical Formula: C54H66Cl2N10O9S2
Exact Mass: 1,132.38
Molecular Weight: 1,134.203
Elemental Analysis: C, 57.19; H, 5.87; Cl, 6.25; N, 12.35; O, 12.70; S, 5.65

Price and Availability

Size	Price	Availability	Quantity
1mg	USD 275
5mg	USD 750
10mg	USD 1250
Bulk inquiry Welcome, Customer: Please do not inquire quote if your intended use is for a patient since our products are for research use and for chemical synthesis use, not for human use . For in-stock products, we listed price in the web page. You may inquire prices for which sizes were not listed. If no price is listed, this means the product is not in stock at the moment, which may be available via custom synthesis. For cost-effective reason, minimum order of 1g is requested (typically very expensive). The lead time is usually 2-4 months. Quote of less than 1g will not be provided. MedKoo may not offer quote for below reasons: (1) current available synthetic method appears to be extremely expensive which is obviously not affordable to most customers; (2) Key raw material to make the product is temporally not available; (3) DEA controlled substances; (4) the product is under patent protection in customer’s country.

Synonym: MT1; MT-1; MT 1; Bis-CPI203-PEG7; Bis-CPI-203-PEG7; (Bis-CPI203)-PEG7;

IUPAC/Chemical Name: N,N'-(3,6,9,12,15,18,21-heptaoxatricosane-1,23-diyl)bis(2-((S)-4-(4-chlorophenyl)-2,3,9-trimethyl-6H-thieno[3,2-f][1,2,4]triazolo[4,3-a][1,4]diazepin-6-yl)acetamide)

InChi Key: JNSLBXJNVHYNNW-CXNSMIOJSA-N

InChi Code: InChI=1S/C54H66Cl2N10O9S2/c1-33-35(3)76-53-47(33)49(39-7-11-41(55)12-8-39)59-43(51-63-61-37(5)65(51)53)31-45(67)57-15-17-69-19-21-71-23-25-73-27-29-75-30-28-74-26-24-72-22-20-70-18-16-58-46(68)32-44-52-64-62-38(6)66(52)54-48(34(2)36(4)77-54)50(60-44)40-9-13-42(56)14-10-40/h7-14,43-44H,15-32H2,1-6H3,(H,57,67)(H,58,68)/t43-,44-/m0/s1

SMILES Code: O=C(NCCOCCOCCOCCOCCOCCOCCOCCNC(C[C@@H](C1=NN=C(N21)C)N=C(C3=C2SC(C)=C3C)C4=CC=C(C=C4)Cl)=O)C[C@H]5C6=NN=C(C)N6C7=C(C(C)=C(C)S7)C(C8=CC=C(Cl)C=C8)=N5

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >3 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Product Data:

Product Data

Instruction:

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Biological target:	MT1 is a bivalent chemical probe of BET bromodomains, with an IC50 of 0.789 nM for BRD4(1).
In vitro activity:	Significant apoptosis was observed by caspase-3 and PARP cleavage after treatment with MT1 (Fig. 5b). These cellular events followed after HEXIM1 upregulation and MYC downregulation. Early and late apoptosis were assessed with annexin-V and propidium iodide staining to compare apoptosis induction between MT1 and JQ1 (Fig. 5c and Supplementary Fig. 12). Importantly, MT1 induced a greater degree of apoptosis at 10-fold lower concentrations than JQ1. Reference: Nat Chem Biol. 2016 Dec;12(12):1089-1096. https://pubmed.ncbi.nlm.nih.gov/27775715/
In vivo activity:	To determine whether MT1 could attenuate the growth of BRD4-dependent leukemia as a single agent in vivo, this study selected an aggressive disseminated leukemia model (mCherry+, Luciferase+, MV4;11) and treated animals with established disease using equimolar (44.2 µmol/kg) and half an equivalent (22.1 µmol/kg) of MT1 compared to JQ1 for 14 days. During the study, leukemic burden was monitored by non-invasive bioluminescence imaging. Even half an equivalent of MT1 significantly reduced leukemic burden over the course of the study compared to either vehicle or JQ1 (Fig. 6a). Post-mortem analysis of leukemic burden in bone marrow by FACS also revealed significantly decreased mCherry+ disease with MT1 administration at 22.1 µg/kg (Fig. 6b). Reference: Nat Chem Biol. 2016 Dec;12(12):1089-1096. https://pubmed.ncbi.nlm.nih.gov/27775715/

Solubility Data

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	150.0	132.25

Preparing Stock Solutions

The following data is based on the product molecular weight 1,134.20 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:	Tanaka M, Roberts JM, Seo HS, Souza A, Paulk J, Scott TG, DeAngelo SL, Dhe-Paganon S, Bradner JE. Design and characterization of bivalent BET inhibitors. Nat Chem Biol. 2016 Dec;12(12):1089-1096. doi: 10.1038/nchembio.2209. Epub 2016 Oct 24. PMID: 27775715; PMCID: PMC5117811.
In vitro protocol:	Tanaka M, Roberts JM, Seo HS, Souza A, Paulk J, Scott TG, DeAngelo SL, Dhe-Paganon S, Bradner JE. Design and characterization of bivalent BET inhibitors. Nat Chem Biol. 2016 Dec;12(12):1089-1096. doi: 10.1038/nchembio.2209. Epub 2016 Oct 24. PMID: 27775715; PMCID: PMC5117811.
In vivo protocol:	Tanaka M, Roberts JM, Seo HS, Souza A, Paulk J, Scott TG, DeAngelo SL, Dhe-Paganon S, Bradner JE. Design and characterization of bivalent BET inhibitors. Nat Chem Biol. 2016 Dec;12(12):1089-1096. doi: 10.1038/nchembio.2209. Epub 2016 Oct 24. PMID: 27775715; PMCID: PMC5117811.

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1: Tanaka M, Roberts JM, Seo HS, Souza A, Paulk J, Scott TG, DeAngelo SL,
Dhe-Paganon S, Bradner JE. Design and characterization of bivalent BET
inhibitors. Nat Chem Biol. 2016 Dec;12(12):1089-1096. doi: 10.1038/nchembio.2209.
Epub 2016 Oct 24. PubMed PMID: 27775715; PubMed Central PMCID: PMC5117811.

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MT1 BET inhibitor featured