Aza-T-dCyd

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MedKoo CAT#: 465873

CAS#: 169514-76-5

Description: Aza-T-dCyd is an orally bioavailable non-toxic "best-in-class" DNA methyltransferase 1 (DNMT1) depleting agent.


Chemical Structure

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Aza-T-dCyd
CAS# 169514-76-5

Theoretical Analysis

MedKoo Cat#: 465873
Name: Aza-T-dCyd
CAS#: 169514-76-5
Chemical Formula: C8H12N4O3S
Exact Mass: 244.06
Molecular Weight: 244.269
Elemental Analysis: C, 39.34; H, 4.95; N, 22.94; O, 19.65; S, 13.12

Price and Availability

This product is not in stock, which may be available by custom synthesis. For cost-effective reason, minimum order is 1g (price is usually high, lead time is 2~3 months, depending on the technical challenge). Quote less than 1g will not be provided. To request quote, please email to sales @medkoo.com or click below button.
Note: Price will be listed if it is available in the future.

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Synonym: Aza-T-dCyd; AzaT-dCyd; Aza T-dCyd; Aza-TdCyd; Aza-T dCyd; Aza T dCyd; AzaTdCyd;

IUPAC/Chemical Name: 4-amino-1-((2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)tetrahydrothiophen-2-yl)-1,3,5-triazin-2(1H)-one

InChi Key: HOOZQNOZVFCJNL-KVQBGUIXSA-N

InChi Code: InChI=1S/C8H12N4O3S/c9-7-10-3-12(8(15)11-7)6-1-4(14)5(2-13)16-6/h3-6,13-14H,1-2H2,(H2,9,11,15)/t4-,5+,6+/m0/s1

SMILES Code: OC[C@@H]1[C@@H](O)C[C@H](N2C=NC(N)=NC2=O)S1

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: To be determined

Shelf Life: >2 years if stored properly

Drug Formulation: To be determined

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Biological target:
In vitro activity:
In vivo activity:

Preparing Stock Solutions

The following data is based on the product molecular weight 244.27 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
In vitro protocol:
In vivo protocol:

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1: Parker WB, Thottassery JV. 5-Aza-4'-thio-2'-deoxycytidine, a New Orally Bioavailable Nontoxic "Best-in-Class": DNA Methyltransferase 1-Depleting Agent in Clinical Development. J Pharmacol Exp Ther. 2021 Nov;379(3):211-222. doi: 10.1124/jpet.121.000758. Epub 2021 Sep 9. PMID: 34503994.

2: Morris J, Wishka DG, Lopez OD, Rudchenko V, Huang G, Hoffman SN, Borgel S, Georgius K, Carter J, Stotler H, Kunkel MW, Collins JM, Hollingshead MG, Teicher BA. F-aza-T-dCyd (NSC801845), a Novel Cytidine Analog, in Comparative Cell Culture and Xenograft Studies with the Clinical Candidates T-dCyd, F-T-dCyd, and Aza-T-dCyd. Mol Cancer Ther. 2021 Apr;20(4):625-631. doi: 10.1158/1535-7163.MCT-20-0738. PMID: 33811149; PMCID: PMC8030693.

3: Wishka DG, Lopez OD, Rudchenko VF, Huang G, Bahde R, Kumar V, Denysenko SM, Zhang L, Zhang M, Teicher BA, Morris J. The development of β-selective glycosylation reactions with benzyl substituted 2-deoxy-1,4-dithio-D-erythro-pentofuranosides: enabling practical multi- gram syntheses of 4'-Thio-2'-deoxycytidine (T-dCyd) and 5-aza-4'-thio-2'-deoxycytidine (aza-T-dCyd) to support clinical development. Nucleosides Nucleotides Nucleic Acids. 2021;40(1):68-95. doi: 10.1080/15257770.2020.1832694. Epub 2020 Oct 16. PMID: 33063584.

4: Thottassery JV, Sambandam V, Allan PW, Maddry JA, Maxuitenko YY, Tiwari K, Hollingshead M, Parker WB. Novel DNA methyltransferase-1 (DNMT1) depleting anticancer nucleosides, 4'-thio-2'-deoxycytidine and 5-aza-4'-thio-2'-deoxycytidine. Cancer Chemother Pharmacol. 2014 Aug;74(2):291-302. doi: 10.1007/s00280-014-2503-z. Epub 2014 Jun 8. PMID: 24908436; PMCID: PMC4194194.