NEM
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MedKoo CAT#: 464946

CAS#: 128-53-0

Description: NEM is a modifier of sulfhydryl groups in proteins. It has commonly been used to inactivate cysteine-containing enzymes in vitro. NEM inhibits prolyl endopeptidase with an IC50 value of 6.3 µM.3 It alkylates cysteine 519 (Cys519) in voltage-gated potassium channel 7.4 (Kv7.4) and activates Kv7.2 , Kv7.4, and Kv7.5, but not Kv7.3, in CHO cells expressing the human channels when used at a concentration of 50 µM.

Chemical Structure

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NEM
CAS# 128-53-0
Instruction

Theoretical Analysis

MedKoo Cat#: 464946
Name: NEM
CAS#: 128-53-0
Chemical Formula: C6H7NO2
Exact Mass: 125.05
Molecular Weight: 125.127
Elemental Analysis: C, 57.59; H, 5.64; N, 11.19; O, 25.57

Price and Availability

Size Price Availability Quantity
5g USD 220 2 Weeks
10g USD 360 2 Weeks
25g USD 590 2 Weeks
50g USD 900 2 Weeks
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Synonym: NEM; NSC 7638; NSC7638; NSC-7638; N-Ethylmaleimide; N Ethylmaleimide;

IUPAC/Chemical Name: 1-ethyl-1H-pyrrole-2,5-dione

InChi Key: HDFGOPSGAURCEO-UHFFFAOYSA-N

InChi Code: InChI=1S/C6H7NO2/c1-2-7-5(8)3-4-6(7)9/h3-4H,2H2,1H3

SMILES Code: O=C1C=CC(N1CC)=O

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: To be determined

Shelf Life: >2 years if stored properly

Drug Formulation: To be determined

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Biological target:
In vitro activity:
In vivo activity:

Preparing Stock Solutions

The following data is based on the product molecular weight 125.13 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
In vitro protocol:
In vivo protocol:

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1: Korotkov SM. Effects of Tl+ on the inner membrane thiol groups, respiration, and swelling in succinate-energized rat liver mitochondria were modified by thiol reagents. Biometals. 2021 Jul 8. doi: 10.1007/s10534-021-00329-6. Epub ahead of print. PMID: 34236558.

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10: Wang P, Wei M, Zhu X, Liu Y, Yoshimura K, Zheng M, Liu G, Kume S, Morishima M, Kurokawa T, Ono K. Nitric oxide down-regulates voltage-gated Na+ channel in cardiomyocytes possibly through S-nitrosylation-mediated signaling. Sci Rep. 2021 May 28;11(1):11273. doi: 10.1038/s41598-021-90840-0. PMID: 34050231; PMCID: PMC8163867.

11: Kim C, Shon MJ, Kim SH, Eun GS, Ryu JK, Hyeon C, Jahn R, Yoon TY. Extreme parsimony in ATP consumption by 20S complexes in the global disassembly of single SNARE complexes. Nat Commun. 2021 May 28;12(1):3206. doi: 10.1038/s41467-021-23530-0. PMID: 34050166; PMCID: PMC8163800.

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13: Davis LJ, Bright NA, Edgar JR, Parkinson MDJ, Wartosch L, Mantell J, Peden AA, Luzio JP. Organelle tethering, pore formation and SNARE compensation in the late endocytic pathway. J Cell Sci. 2021 May 15;134(10):jcs255463. doi: 10.1242/jcs.255463. Epub 2021 May 27. PMID: 34042162; PMCID: PMC8186482.

14: Moro A, van Nifterick A, Toonen RF, Verhage M. Dynamin controls neuropeptide secretion by organizing dense-core vesicle fusion sites. Sci Adv. 2021 May 21;7(21):eabf0659. doi: 10.1126/sciadv.abf0659. PMID: 34020952; PMCID: PMC8139595.

15: Qian L, Yang X, Li S, Zhao H, Gao Y, Zhao S, Lv X, Zhang X, Li L, Zhai L, Zhou F, Chen B. Reduced O-GlcNAcylation of SNAP-23 promotes cisplatin resistance by inducing exosome secretion in ovarian cancer. Cell Death Discov. 2021 May 18;7(1):112. doi: 10.1038/s41420-021-00489-x. PMID: 34001861; PMCID: PMC8128872.

16: Pulukool SK, Bhagavatham SKS, Kannan V, Sukumar P, Dandamudi RB, Ghaisas S, Kunchala H, Saieesh D, Naik AA, Pargaonkar A, Sharma A, Sivaramakrishnan V. Elevated dimethylarginine, ATP, cytokines, metabolic remodeling involving tryptophan metabolism and potential microglial inflammation characterize primary open angle glaucoma. Sci Rep. 2021 May 7;11(1):9766. doi: 10.1038/s41598-021-89137-z. PMID: 33963197; PMCID: PMC8105335.

17: Young KZ, Cartee NMP, Lee SJ, Keep SG, Ivanova MI, Wang MM. Electrophilic and Drug-Induced Stimulation of NOTCH3 N-terminal Fragment Oligomerization in Cerebrovascular Pathology. Transl Stroke Res. 2021 May 3. doi: 10.1007/s12975-021-00908-2. Epub ahead of print. PMID: 33939102.

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20: Liu F, Li JP, Li LS, Liu Q, Li SW, Song ML, Li S, Zhang Y. The canonical α-SNAP is essential for gametophytic development in Arabidopsis. PLoS Genet. 2021 Apr 22;17(4):e1009505. doi: 10.1371/journal.pgen.1009505. PMID: 33886546; PMCID: PMC8096068.