Fluorescein dipotassium

    WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 575584

CAS#: 6417-85-2

Description: Fluorescein dipotassium is a colorimetric probe formed by the reaction of adipic acid dihydrazide and fluorescein isothiocyanate.

Chemical Structure

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Fluorescein dipotassium
CAS# 6417-85-2
Instruction

Theoretical Analysis

MedKoo Cat#: 575584
Name: Fluorescein dipotassium
CAS#: 6417-85-2
Chemical Formula: C20H10K2O5
Exact Mass: 407.98
Molecular Weight: 408.490
Elemental Analysis: C, 58.81; H, 2.47; K, 19.14; O, 19.58

Price and Availability

This product is not in stock, which may be available by custom synthesis. For cost-effective reason, minimum order is 1g (price is usually high, lead time is 2~3 months, depending on the technical challenge). Quote less than 1g will not be provided. To request quote, please email to sales @medkoo.com or click below button.
Note: Price will be listed if it is available in the future.

Request quote for custom synthesis

Synonym: Fluorescein dipotassium

IUPAC/Chemical Name: Spiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one, 3',6'-dihydroxy-, dipotassium salt

InChi Key: DZSPMLRDMGJVAT-UHFFFAOYSA-L

InChi Code: InChI=1S/C20H12O5.2K/c21-11-5-7-15-17(9-11)24-18-10-12(22)6-8-16(18)20(15)14-4-2-1-3-13(14)19(23)25-20;;/h1-10,21-22H;;/q;2*+1/p-2

SMILES Code: [K+].[K+].[O-]c1ccc2c(Oc3cc([O-])ccc3C24OC(=O)c5ccccc45)c1

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Biological target:
In vitro activity:
In vivo activity:

Preparing Stock Solutions

The following data is based on the product molecular weight 408.49 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
In vitro protocol:
In vivo protocol:

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1. Zhang L, Zhang X. A selectively fluorescein-based colorimetric probe for detecting copper(II) ion. Spectrochim Acta A Mol Biomol Spectrosc. 2014 Dec 10;133:54-9. doi: 10.1016/j.saa.2014.04.130. Epub 2014 Apr 30. PMID: 24929315.

2. Ahn B, Rhee SG, Stadtman ER. Use of fluorescein hydrazide and fluorescein thiosemicarbazide reagents for the fluorometric determination of protein carbonyl groups and for the detection of oxidized protein on polyacrylamide gels. Anal Biochem. 1987 Mar;161(2):245-57. doi: 10.1016/0003-2697(87)90448-9. PMID: 2883911.

3. Zhang J, Zhang L, Wei Y, Ma J, Shuang S, Cai Z, Dong C. A selectively fluorescein-based colorimetric probe for detecting copper(II) ion. Spectrochim Acta A Mol Biomol Spectrosc. 2014 Mar 25;122:731-6. doi: 10.1016/j.saa.2013.11.096. Epub 2013 Nov 23. PMID: 24370938.