Fluo-3
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    WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 575570

CAS#: 123632-39-3

Description: Fluo-3 is a fluorescent Ca(2+) indicator that permits continuous monitoring of Ca without interference with use of UV-sensitive caged compounds.

Chemical Structure

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Fluo-3
CAS# 123632-39-3
Instruction

Theoretical Analysis

MedKoo Cat#: 575570
Name: Fluo-3
CAS#: 123632-39-3
Chemical Formula: C36H30Cl2N2O13
Exact Mass: 768.11
Molecular Weight: 769.540
Elemental Analysis: C, 56.19; H, 3.93; Cl, 9.21; N, 3.64; O, 27.03

Price and Availability

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1mg USD 535 2 Weeks
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Synonym: Fluo 3; Fluo3; Fluo-3; Fluo-3-AM

IUPAC/Chemical Name: Glycine, N-(2-(2-(2-(bis(carboxymethyl)amino)-5-(2,7-dichloro-6-hydroxy-3-oxo-3H-xanthene-9-yl)phenoxy)ethoxy)-4-methylphenyl)-N-(carboxymethyl)-

InChi Key: OZLGRUXZXMRXGP-UHFFFAOYSA-N

InChi Code: InChI=1S/C36H30Cl2N2O13/c1-18-2-4-24(39(14-32(43)44)15-33(45)46)30(8-18)51-6-7-52-31-9-19(3-5-25(31)40(16-34(47)48)17-35(49)50)36-20-10-22(37)26(41)12-28(20)53-29-13-27(42)23(38)11-21(29)36/h2-5,8-13,41H,6-7,14-17H2,1H3,(H,43,44)(H,45,46)(H,47,48)(H,49,50)

SMILES Code: Cc1ccc(N(CC(=O)O)CC(=O)O)c(OCCOc2cc(ccc2N(CC(=O)O)CC(=O)O)C3=C4C=C(Cl)C(=O)C=C4Oc5cc(O)c(Cl)cc35)c1

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Biological target:
In vitro activity:
In vivo activity:

Preparing Stock Solutions

The following data is based on the product molecular weight 769.54 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
In vitro protocol:
In vivo protocol:

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1. Zhang S, Li C, Gao J, Qiu X, Cui Z. [Application of the Ca2+ indicator fluo-3 and fluo-4 in the process of H2O2 induced apoptosis of A549 cell]. Zhongguo Fei Ai Za Zhi. 2014 Mar;17(3):197-202. Chinese. doi: 10.3779/j.issn.1009-3419.2014.03.03. PMID: 24667255; PMCID: PMC6019359.

2. Timmers AC, Reiss HD, Schel JH. Digitonin-aided loading of Fluo-3 into embryogenic plant cells. Cell Calcium. 1991 Jul;12(7):515-21. doi: 10.1016/0143-4160(91)90033-b. PMID: 1934038.

3. Merritt JE, McCarthy SA, Davies MP, Moores KE. Use of fluo-3 to measure cytosolic Ca2+ in platelets and neutrophils. Loading cells with the dye, calibration of traces, measurements in the presence of plasma, and buffering of cytosolic Ca2+. Biochem J. 1990 Jul 15;269(2):513-9. doi: 10.1042/bj2690513. PMID: 2117443; PMCID: PMC1131607.