Resorufin sodium salt
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MedKoo CAT#: 462973

CAS#: 34994-50-8

Description: Resorufin is a fluorescent probe and the reduced form of the redox indicator dye resazurin. It can be further reduced to hydroresorufin, which is non-fluorescent. Resorufin has been used in the synthesis of resorufin-based fluorescent probes. It has also been used for the direct measurement of solvent-solute hydrogen bond dynamics with distinct absorption and emission peaks in solvents with strong hydrogen bonds and overlapping peaks in solvents with weak hydrogen bonds. It displays excitation/emission maxima of 570/580 nm, respectively.

Chemical Structure

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Resorufin sodium salt
CAS# 34994-50-8
Product data Instruction

Theoretical Analysis

MedKoo Cat#: 462973
Name: Resorufin sodium salt
CAS#: 34994-50-8
Chemical Formula: C12H6NNaO3
Exact Mass: 0.00
Molecular Weight: 235.170
Elemental Analysis: C, 61.29; H, 2.57; N, 5.96; Na, 9.78; O, 20.41

Price and Availability

Size Price Availability Quantity
10mg USD -2 2 Weeks
25mg USD -2 2 Weeks
50mg USD -2 2 Weeks
2g USD -2 2 Weeks
5g USD -2 2 Weeks
100mg USD 220 2 Weeks
250mg USD 360 2 Weeks
500mg USD 580 2 Weeks
1g USD 880 2 Weeks
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Synonym: Resorufin sodium salt; Resorufin;

IUPAC/Chemical Name: sodium 3-oxo-3H-phenoxazin-7-olate

InChi Key: TYXBZZBYMSVFPR-UHFFFAOYSA-M

InChi Code: InChI=1S/C12H7NO3.Na/c14-7-1-3-9-11(5-7)16-12-6-8(15)2-4-10(12)13-9;/h1-6,14H;/q;+1/p-1

SMILES Code: O=C1C=C2OC3=CC([O-])=CC=C3N=C2C=C1.[Na+]

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO and water

Shelf Life: >3 years if stored properly

Drug Formulation: This drug may be formulated in DMSO and water

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Product Data:
Product Data
Instruction:
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Biological target: Resorufin has been used to determine the cytochrome P450 2A5 coumarin 7-hydroxylase activity changes over a broad range of lipopolysaccharide doses. It has also been used as an internal standard for ethoxyresurufin-o-deethylase analysis.
In vitro activity: An iodinated resorufin derivative (R1) induced photocytotoxicity after being triggered by endogenous monoamine oxidase (MAO) enzyme in cancer cells. R1 displayed differential cytotoxicity between cancer and normal cells, without any considerable dark toxicity. R1 marks the first example of a resorufin-based photosensitizer as well as the first anticancer drug that is activated by a MAO enzyme. Reference: ACS Med Chem Lett. 2020 Nov 23;11(12):2491-2496. https://pubmed.ncbi.nlm.nih.gov/33335672/
In vivo activity: This study used a resorufin-based fluorescence probe to sense various biogenic amines (Bas). Upon nucleophilic substitution reaction with BAs, the probe released resorufin, affording to strong fluorescence emission at 592 nm with rapid response (<8 min), good selectivity and a low detection limit (LOD = 0.47 μM). Resorufin has low cytotoxicity and good membrane permeability; it has been successfully used to visualize BAs in living cells and zebrafish with good performance. Reference: RSC Adv. 2022 Nov 25;12(52):33870-33875. https://pubmed.ncbi.nlm.nih.gov/36505703/

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 62.5 265.77
Water 7.7 32.70

Preparing Stock Solutions

The following data is based on the product molecular weight 235.17 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol: 1. Almammadov T, Atakan G, Leylek O, Ozcan G, Gunbas G, Kolemen S. Resorufin Enters the Photodynamic Therapy Arena: A Monoamine Oxidase Activatable Agent for Selective Cytotoxicity. ACS Med Chem Lett. 2020 Nov 23;11(12):2491-2496. doi: 10.1021/acsmedchemlett.0c00484. PMID: 33335672; PMCID: PMC7734820. 2. Sandholt IS, Olsen BB, Guerra B, Issinger OG. Resorufin: a lead for a new protein kinase CK2 inhibitor. Anticancer Drugs. 2009 Apr;20(4):238-48. doi: 10.1097/CAD.0b013e328326472e. PMID: 19177021. 3. Pei SL, Zhang J, Ge W, Liu C, Sheng R, Zeng L, Pan LH. A resorufin-based fluorescence probe for visualizing biogenic amines in cells and zebrafish. RSC Adv. 2022 Nov 25;12(52):33870-33875. doi: 10.1039/d2ra06482k. PMID: 36505703; PMCID: PMC9693732. 4. Su H, Wang N, Wang J, Wang H, Zhang J, Zhao W. A resorufin-based red-emitting fluorescent probe with high selectivity for tracking endogenous peroxynitrite in living cells and inflammatory mice. Spectrochim Acta A Mol Biomol Spectrosc. 2021 May 5;252:119502. doi: 10.1016/j.saa.2021.119502. Epub 2021 Jan 30. PMID: 33578120.
In vitro protocol: 1. Almammadov T, Atakan G, Leylek O, Ozcan G, Gunbas G, Kolemen S. Resorufin Enters the Photodynamic Therapy Arena: A Monoamine Oxidase Activatable Agent for Selective Cytotoxicity. ACS Med Chem Lett. 2020 Nov 23;11(12):2491-2496. doi: 10.1021/acsmedchemlett.0c00484. PMID: 33335672; PMCID: PMC7734820. 2. Sandholt IS, Olsen BB, Guerra B, Issinger OG. Resorufin: a lead for a new protein kinase CK2 inhibitor. Anticancer Drugs. 2009 Apr;20(4):238-48. doi: 10.1097/CAD.0b013e328326472e. PMID: 19177021.
In vivo protocol: 1. Pei SL, Zhang J, Ge W, Liu C, Sheng R, Zeng L, Pan LH. A resorufin-based fluorescence probe for visualizing biogenic amines in cells and zebrafish. RSC Adv. 2022 Nov 25;12(52):33870-33875. doi: 10.1039/d2ra06482k. PMID: 36505703; PMCID: PMC9693732. 2. Su H, Wang N, Wang J, Wang H, Zhang J, Zhao W. A resorufin-based red-emitting fluorescent probe with high selectivity for tracking endogenous peroxynitrite in living cells and inflammatory mice. Spectrochim Acta A Mol Biomol Spectrosc. 2021 May 5;252:119502. doi: 10.1016/j.saa.2021.119502. Epub 2021 Jan 30. PMID: 33578120.

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