EdU (5-ethynyl-2'-deoxyuridine)
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    WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 558934

CAS#: 61135-33-9

Description: EdU (ethynyldeoxyuridine) is a nucleoside, an analog of thymidine, carrying an ethynyl group. This nucleoside behaves as a substrate for cellular DNA replication machinery. Then, DNA containing ethynyl groups can be developed by Click chemistry reaction with various dye azides. Fluorescent DNA can be detected by microscopy, or cells can be sorted by FACS.


Chemical Structure

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EdU (5-ethynyl-2'-deoxyuridine)
CAS# 61135-33-9

Theoretical Analysis

MedKoo Cat#: 558934
Name: EdU (5-ethynyl-2'-deoxyuridine)
CAS#: 61135-33-9
Chemical Formula: C11H12N2O5
Exact Mass: 252.0746
Molecular Weight: 252.23
Elemental Analysis: C, 52.38; H, 4.80; N, 11.11; O, 31.72

Price and Availability

This product is not in stock, which may be available by custom synthesis. For cost-effective reason, minimum order is 1g (price is usually high, lead time is 2~3 months, depending on the technical challenge). Quote less than 1g will not be provided. To request quote, please email to sales @medkoo.com or click below button.
Note: Price will be listed if it is available in the future.

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Synonym:

IUPAC/Chemical Name: 5-ethynyl-1-((2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)pyrimidine-2,4(1H,3H)-dione

InChi Key: CDEURGJCGCHYFH-DJLDLDEBSA-N

InChi Code: InChI=1S/C11H12N2O5/c1-2-6-4-13(11(17)12-10(6)16)9-3-7(15)8(5-14)18-9/h1,4,7-9,14-15H,3,5H2,(H,12,16,17)/t7-,8+,9+/m0/s1

SMILES Code: O=C(NC1=O)N(C=C1C#C)[C@@H]2O[C@H](CO)[C@@H](O)C2

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >3 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

Preparing Stock Solutions

The following data is based on the product molecular weight 252.23 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL

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3. Wang, Z.; Cui, M.; Shah, A.M.; Ye, W.; Tan, W.; Min, Y.-L.; Botten, G.A.; Shelton, J.M.; Liu, N.; Bassel-Duby, R.; Olson, E.N. Mechanistic basis of neonatal heart regeneration revealed by transcriptome and histone modification profiling. Proceedings of the National Academy of Sciences of the U.S.A., 2019, 116(37), 18455–18465. doi: 10.1073/pnas.1905824116

4. Morais, C.M.; Cunha, P.P.; Melo, T.; Cardoso, A.M.; Domingues, P.; Domingues, M.R.; Pedroso de Lima, M.C.; Jurado, A.S. Glucosylceramide synthase silencing combined with the receptor tyrosine kinase inhibitor axitinib as a new multimodal strategy for glioblastoma. Human Molecular Genetics, 2019, 28(21), 3664–3679. doi: 10.1093/hmg/ddz152