L012 sodium

    WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 563087

CAS#: 143556-24-5

Description: L012 sodium is a chemiluminescent probe for detecting NAPDH oxidase-derived reactive oxygen and nitrogen species (ROS and RNS).


Price and Availability

Size
Price

Size
Price

Size
Price

L012 sodium is not in stock, may be available through custom synthesis. For cost-effective reason, minimum 1 gram order is requested. The product will be characterized by NMR, HPLC and MS analysis. Purity (HPLC) is usually >98%. CoA, QC data, MSDS will be provided when product is successfully made. The estimated lead time is 2-3 months. Please send email to sales@medkoo.com to inquire quote.


Chemical Structure

img

Theoretical Analysis

MedKoo Cat#: 563087
Name: L012 sodium
CAS#: 143556-24-5
Chemical Formula: C13H8ClN4NaO2
Exact Mass: 310.0233
Molecular Weight: 310.67
Elemental Analysis: C, 50.26; H, 2.60; Cl, 11.41; N, 18.03; Na, 7.40; O, 10.30


Synonym: L012 sodium salt; L012 sodium; L-012 sodium; L 012 sodium; L 012 sodium salt; L-012 sodium salt;

IUPAC/Chemical Name: sodium 8-amino-5-chloro-4-oxo-7-phenyl-3,4-dihydropyrido[3,4-d]pyridazin-1-olate

InChi Key: IGEUYSJHQQCEFP-UHFFFAOYSA-M

InChi Code: InChI=1S/C13H9ClN4O2.Na/c14-11-8-7(12(19)17-18-13(8)20)9(15)10(16-11)6-4-2-1-3-5-6;/h1-5H,15H2,(H,17,19)(H,18,20);/q;+1/p-1

SMILES Code: O=C1C(C(Cl)=NC(C2=CC=CC=C2)=C3N)=C3C(O[Na])=NN1


Technical Data

Appearance:
Solid powder

Purity:
>98% (or refer to the Certificate of Analysis)

Shipping Condition:
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition:
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility:
Soluble in DMSO

Shelf Life:
>2 years if stored properly

Drug Formulation:
This drug may be formulated in DMSO

Stock Solution Storage:
0 - 4 C for short term (days to weeks), or -20 C for long term (months).

Harmonized System Code:
293490


References

1: Prieto-Lloret J, Snetkov VA, Shaifta Y, Docio I, Connolly MJ, MacKay CE, Knock GA, Ward JPT, Aaronson PI. Role of reactive oxygen species and sulfide-quinone oxoreductase in hydrogen sulfide-induced contraction of rat pulmonary arteries. Am J Physiol Lung Cell Mol Physiol. 2018 Apr 1;314(4):L670-L685. doi: 10.1152/ajplung.00283.2016. Epub 2017 Dec 28. PubMed PMID: 29351439; PubMed Central PMCID: PMC5966778.

2: Filipowicz N, Momotko M, Boczkaj G, Pawlikowski T, Wanarska M, Cieśliński H. Isolation and Characterization of Phenol-Degrading Psychrotolerant Yeasts. Water Air Soil Pollut. 2017;228(6):210. doi: 10.1007/s11270-017-3391-8. Epub 2017 May 22. PubMed PMID: 28603316; PubMed Central PMCID: PMC5440478.

3: Cui C, Chen Y, Jiang D, Zhu JJ, Chen HY. Attomole Antigen Detection Using Self-Electrochemiluminous Graphene Oxide-Capped Au@L012 Nanocomposite. Anal Chem. 2017 Feb 21;89(4):2418-2423. doi: 10.1021/acs.analchem.6b04305. Epub 2017 Feb 9. PubMed PMID: 28192953.

4: Melcher RL, Moerschbacher BM. An improved microtiter plate assay to monitor the oxidative burst in monocot and dicot plant cell suspension cultures. Plant Methods. 2016 Jan 26;12:5. doi: 10.1186/s13007-016-0110-1. eCollection 2016. PubMed PMID: 26819624; PubMed Central PMCID: PMC4729151.

5: Rezende F, Löwe O, Helfinger V, Prior KK, Walter M, Zukunft S, Fleming I, Weissmann N, Brandes RP, Schröder K. Unchanged NADPH Oxidase Activity in Nox1-Nox2-Nox4 Triple Knockout Mice: What Do NADPH-Stimulated Chemiluminescence Assays Really Detect? Antioxid Redox Signal. 2016 Mar 1;24(7):392-9. doi: 10.1089/ars.2015.6314. Epub 2015 Jun 30. Review. PubMed PMID: 25906178.

6: Pingel S, Tiyerili V, Mueller J, Werner N, Nickenig G, Mueller C. Thrombin inhibition by dabigatran attenuates atherosclerosis in ApoE deficient mice. Arch Med Sci. 2014 Feb 24;10(1):154-60. doi: 10.5114/aoms.2014.40742. Epub 2014 Feb 23. PubMed PMID: 24701228; PubMed Central PMCID: PMC3953984.

7: Hoyer FF, Khoury M, Slomka H, Kebschull M, Lerner R, Lutz B, Schott H, Lütjohann D, Wojtalla A, Becker A, Zimmer A, Nickenig G. Inhibition of endocannabinoid-degrading enzyme fatty acid amide hydrolase increases atherosclerotic plaque vulnerability in mice. J Mol Cell Cardiol. 2014 Jan;66:126-32. doi: 10.1016/j.yjmcc.2013.11.013. Epub 2013 Nov 25. PubMed PMID: 24286707.

8: Ondrackova P, Alexa P, Matiasovic J, Volf J, Faldyna M. Interaction of porcine neutrophils with different strains of enterotoxigenic Escherichia coli. Vet Microbiol. 2012 Nov 9;160(1-2):108-16. doi: 10.1016/j.vetmic.2012.05.010. Epub 2012 May 28. PubMed PMID: 22704243.

9: Hoyer FF, Steinmetz M, Zimmer S, Becker A, Lütjohann D, Buchalla R, Zimmer A, Nickenig G. Atheroprotection via cannabinoid receptor-2 is mediated by circulating and vascular cells in vivo. J Mol Cell Cardiol. 2011 Dec;51(6):1007-14. doi: 10.1016/j.yjmcc.2011.08.008. Epub 2011 Aug 18. PubMed PMID: 21884703.

10: Hoyer FF, Albrecht L, Nickenig G, Müller C. Selective inhibition of leukotriene receptor BLT-2 reduces vascular oxidative stress and improves endothelial function in ApoE-/- mice. Mol Cell Biochem. 2012 Jan;359(1-2):25-31. doi: 10.1007/s11010-011-0995-y. Epub 2011 Jul 14. PubMed PMID: 21755457.

11: Becher UM, Ghanem A, Tiyerili V, Fürst DO, Nickenig G, Mueller CF. Inhibition of leukotriene C4 action reduces oxidative stress and apoptosis in cardiomyocytes and impedes remodeling after myocardial injury. J Mol Cell Cardiol. 2011 Mar;50(3):570-7. doi: 10.1016/j.yjmcc.2010.11.013. Epub 2010 Nov 26. PubMed PMID: 21112334.

12: Judkins CP, Diep H, Broughton BR, Mast AE, Hooker EU, Miller AA, Selemidis S, Dusting GJ, Sobey CG, Drummond GR. Direct evidence of a role for Nox2 in superoxide production, reduced nitric oxide bioavailability, and early atherosclerotic plaque formation in ApoE-/- mice. Am J Physiol Heart Circ Physiol. 2010 Jan;298(1):H24-32. doi: 10.1152/ajpheart.00799.2009. Epub 2009 Oct 16. PubMed PMID: 19837950.

13: Mueller CF, Becher MU, Zimmer S, Wassmann S, Keuler B, Nickenig G. Angiotensin II triggers release of leukotriene C4 in vascular smooth muscle cells via the multidrug resistance-related protein 1. Mol Cell Biochem. 2010 Jan;333(1-2):261-7. doi: 10.1007/s11010-009-0227-x. Epub 2009 Aug 15. PubMed PMID: 19685171.

14: Imada I, Sato EF, Kira Y, Inoue M. Effect of CoQ homologues on reactive oxygen generation by mitochondria. Biofactors. 2008;32(1-4):41-8. PubMed PMID: 19096099.

15: Jabs A, Göbel S, Wenzel P, Kleschyov AL, Hortmann M, Oelze M, Daiber A, Münzel T. Sirolimus-induced vascular dysfunction. Increased mitochondrial and nicotinamide adenosine dinucleotide phosphate oxidase-dependent superoxide production and decreased vascular nitric oxide formation. J Am Coll Cardiol. 2008 Jun 3;51(22):2130-8. doi: 10.1016/j.jacc.2008.01.058. PubMed PMID: 18510959.

16: Mueller CF, Wassmann K, Widder JD, Wassmann S, Chen CH, Keuler B, Kudin A, Kunz WS, Nickenig G. Multidrug resistance protein-1 affects oxidative stress, endothelial dysfunction, and atherogenesis via leukotriene C4 export. Circulation. 2008 Jun 3;117(22):2912-8. doi: 10.1161/CIRCULATIONAHA.107.747667. Epub 2008 May 27. PubMed PMID: 18506003.

17: Kadomura K, Naruse S, Sugihara S, Yamaguchi K, Oda T. Production of reactive oxygen species (ROS) by various marine fish species during the larval stage. Biosci Biotechnol Biochem. 2007 Aug;71(8):2029-33. Epub 2007 Aug 7. PubMed PMID: 17690462.

18: Ambasta RK, Schreiber JG, Janiszewski M, Busse R, Brandes RP. Noxa1 is a central component of the smooth muscle NADPH oxidase in mice. Free Radic Biol Med. 2006 Jul 15;41(2):193-201. Epub 2006 Jan 30. PubMed PMID: 16814099.