RCM-1
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    WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 525344

CAS#: 339163-65-4

Description: RCM-1 is a nontoxic FOXM1 inhibitor, inhibiting goblet cell metaplasia and excessive mucus production.

Chemical Structure

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RCM-1
CAS# 339163-65-4
Product data Instruction

Theoretical Analysis

MedKoo Cat#: 525344
Name: RCM-1
CAS#: 339163-65-4
Chemical Formula: C20H12N2OS4
Exact Mass: 423.98
Molecular Weight: 424.570
Elemental Analysis: C, 56.58; H, 2.85; N, 6.60; O, 3.77; S, 30.20

Price and Availability

Size Price Availability Quantity
5mg USD 325
10mg USD 565
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Synonym: RCM-1; RCM1; RCM 1.

IUPAC/Chemical Name: 2-{[2-Oxo-2-(thiophen-2-yl)ethyl]sulfanyl}-4,6-di(thiophen-2-yl)pyridine-3- carbonitrile

InChi Key: DCCYTKKDAZEVLY-UHFFFAOYSA-N

InChi Code: InChI=1S/C20H12N2OS4/c21-11-14-13(17-4-1-7-24-17)10-15(18-5-2-8-25-18)22-20(14)27-12-16(23)19-6-3-9-26-19/h1-10H,12H2

SMILES Code: N#CC1=C(C2=CC=CS2)C=C(C3=CC=CS3)N=C1SCC(C4=CC=CS4)=O

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Product Data:
Product Data
Certificate of Analysis:
View CoA: current batch, Lot#AOB80925
Safety Data Sheet (SDS):
View Safety Data Sheet (SDS)
Instruction:
View handling instruction
Biological target: RCM 1 is a forkhead box M1 (FOXM1) transcription factor inhibitor (IC50 = 0.72 μM). Inhibits expression of FOXM1 target genes. Induces FOXM1 protein degradation in vitro. Reduces airway resistance, increases lung compliance and decreases proinflammatory cytokine production in allergen-exposed mice.
In vitro activity: RCM-1 decreased protein levels and nuclear localization of β-catenin, and inhibited protein-protein interaction between β-catenin and FOXM1 in cultured tumor cells. RCM-1 could be a promising candidate for anticancer therapy. Reference: Mol Cancer Ther. 2019 Jul;18(7):1217-1229. https://pubmed.ncbi.nlm.nih.gov/31040162/
In vivo activity: The results of this study suggest that RCM-1 is an inhibitor of goblet cell metaplasia and IL-13 signaling. These results provide a new therapeutic candidate to treat patients with asthma and other chronic airway diseases. Reference: Sci Signal. 2017 Apr 18;10(475):eaai8583. https://pubmed.ncbi.nlm.nih.gov/28420758/

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 8.5 20.00

Preparing Stock Solutions

The following data is based on the product molecular weight 424.57 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol: 1. Kamimura M, Sasaki A, Otani Y, Nakamura Y, Nakamura T, Kuramochi K, Imai T, Kubo N, Okamoto S. Methylthioacetic acid, a derivative of aroma compounds from Cucumis melo var. conomon dose-dependently triggers differentiation and apoptosis of RCM-1 human colorectal cancer cells. J Toxicol Sci. 2023;48(1):25-35. doi: 10.2131/jts.48.25. PMID: 36599425. 2. Shukla S, Milewski D, Pradhan A, Rama N, Rice K, Le T, Flick MJ, Vaz S, Zhao X, Setchell KD, Logarinho E, Kalinichenko VV, Kalin TV. The FOXM1 Inhibitor RCM-1 Decreases Carcinogenesis and Nuclear β-Catenin. Mol Cancer Ther. 2019 Jul;18(7):1217-1229. doi: 10.1158/1535-7163.MCT-18-0709. Epub 2019 Apr 30. PMID: 31040162; PMCID: PMC7341442. 3. Sun L, Ren X, Wang IC, Pradhan A, Zhang Y, Flood HM, Han B, Whitsett JA, Kalin TV, Kalinichenko VV. The FOXM1 inhibitor RCM-1 suppresses goblet cell metaplasia and prevents IL-13 and STAT6 signaling in allergen-exposed mice. Sci Signal. 2017 Apr 18;10(475):eaai8583. doi: 10.1126/scisignal.aai8583. PMID: 28420758.
In vitro protocol: 1. Kamimura M, Sasaki A, Otani Y, Nakamura Y, Nakamura T, Kuramochi K, Imai T, Kubo N, Okamoto S. Methylthioacetic acid, a derivative of aroma compounds from Cucumis melo var. conomon dose-dependently triggers differentiation and apoptosis of RCM-1 human colorectal cancer cells. J Toxicol Sci. 2023;48(1):25-35. doi: 10.2131/jts.48.25. PMID: 36599425. 2. Shukla S, Milewski D, Pradhan A, Rama N, Rice K, Le T, Flick MJ, Vaz S, Zhao X, Setchell KD, Logarinho E, Kalinichenko VV, Kalin TV. The FOXM1 Inhibitor RCM-1 Decreases Carcinogenesis and Nuclear β-Catenin. Mol Cancer Ther. 2019 Jul;18(7):1217-1229. doi: 10.1158/1535-7163.MCT-18-0709. Epub 2019 Apr 30. PMID: 31040162; PMCID: PMC7341442.
In vivo protocol: 1. Sun L, Ren X, Wang IC, Pradhan A, Zhang Y, Flood HM, Han B, Whitsett JA, Kalin TV, Kalinichenko VV. The FOXM1 inhibitor RCM-1 suppresses goblet cell metaplasia and prevents IL-13 and STAT6 signaling in allergen-exposed mice. Sci Signal. 2017 Apr 18;10(475):eaai8583. doi: 10.1126/scisignal.aai8583. PMID: 28420758.

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1: Sun L, Ren X, Wang IC, Pradhan A, Zhang Y, Flood HM, Han B, Whitsett JA, Kalin TV, Kalinichenko VV. The FOXM1 inhibitor RCM-1 suppresses goblet cell metaplasia and prevents IL-13 and STAT6 signaling in allergen-exposed mice. Sci Signal. 2017 Apr 18;10(475). pii: eaai8583. doi: 10.1126/scisignal.aai8583. PubMed PMID: 28420758.

2: De Sousa PA, Tye BJ, Sneddon S, Bruce K, Dand P, Russell G, Collins DM, Greenshields A, McDonald K, Bradburn H, Gardner J, Downie JM, Courtney A, Brison DR. Derivation of the human embryonic stem cell line RCM1. Stem Cell Res. 2016 Mar;16(2):476-80. doi: 10.1016/j.scr.2015.12.020. Epub 2015 Dec 31. PubMed PMID: 27346018.

3: Cupertino FB, Virgilio S, Freitas FZ, Candido Tde S, Bertolini MC. Regulation of glycogen metabolism by the CRE-1, RCO-1 and RCM-1 proteins in Neurospora crassa. The role of CRE-1 as the central transcriptional regulator. Fungal Genet Biol. 2015 Apr;77:82-94. doi: 10.1016/j.fgb.2015.03.011. Epub 2015 Apr 16. PubMed PMID: 25889113.

4: Liu X, Li H, Liu Q, Niu Y, Hu Q, Deng H, Cha J, Wang Y, Liu Y, He Q. Role for Protein Kinase A in the Neurospora Circadian Clock by Regulating White Collar-Independent frequency Transcription through Phosphorylation of RCM-1. Mol Cell Biol. 2015 Jun;35(12):2088-102. doi: 10.1128/MCB.00709-14. Epub 2015 Apr 6. PubMed PMID: 25848091; PubMed Central PMCID: PMC4438235.

5: Png CW, Weerasooriya M, Guo J, James SJ, Poh HM, Osato M, Flavell RA, Dong C, Yang H, Zhang Y. DUSP10 regulates intestinal epithelial cell growth and colorectal tumorigenesis. Oncogene. 2016 Jan 14;35(2):206-17. doi: 10.1038/onc.2015.74. Epub 2015 Mar 16. PubMed PMID: 25772234.

6: Saeki T, Inui H, Fujioka S, Fukuda S, Nomura A, Nakamura Y, Park EY, Sato K, Kanamoto R. Staurosporine synergistically potentiates the deoxycholate-mediated induction of COX-2 expression. Physiol Rep. 2014 Aug 28;2(8). pii: e12143. doi: 10.14814/phy2.12143. Print 2014 Aug 1. PubMed PMID: 25168879; PubMed Central PMCID: PMC4246598.

7: Ruger-Herreros C, Gil-Sánchez Mdel M, Sancar G, Brunner M, Corrochano LM. Alteration of light-dependent gene regulation by the absence of the RCO-1/RCM-1 repressor complex in the fungus Neurospora crassa. PLoS One. 2014 Apr 18;9(4):e95069. doi: 10.1371/journal.pone.0095069. eCollection 2014. PubMed PMID: 24747913; PubMed Central PMCID: PMC3991626.

8: Koller S, Inzinger M, Rothmund M, Ahlgrimm-Siess V, Massone C, Arzberger E, Wolf P, Hofmann-Wellenhof R. UV-induced alterations of the skin evaluated over time by reflectance confocal microscopy. J Eur Acad Dermatol Venereol. 2014 Aug;28(8):1061-8. doi: 10.1111/jdv.12284. Epub 2013 Oct 17. PubMed PMID: 24131408.

9: Nakamura Y, Watanabe S, Kageyama M, Shirota K, Shirota K, Amano H, Kashimoto T, Matsuo T, Okamoto S, Park EY, Sato K. Antimutagenic; differentiation-inducing; and antioxidative effects of fragrant ingredients in Katsura-uri (Japanese pickling melon; Cucumis melo var. conomon). Mutat Res. 2010 Dec 21;703(2):163-8. doi: 10.1016/j.mrgentox.2010.08.015. Epub 2010 Aug 27. PubMed PMID: 20801232.

10: Olmedo M, Navarro-Sampedro L, Ruger-Herreros C, Kim SR, Jeong BK, Lee BU, Corrochano LM. A role in the regulation of transcription by light for RCO-1 and RCM-1, the Neurospora homologs of the yeast Tup1-Ssn6 repressor. Fungal Genet Biol. 2010 Nov;47(11):939-52. doi: 10.1016/j.fgb.2010.08.001. Epub 2010 Aug 10. PubMed PMID: 20709620.

11: Aldabbous MS, Roca MG, Stout A, Huang IC, Read ND, Free SJ. The ham-5, rcm-1 and rco-1 genes regulate hyphal fusion in Neurospora crassa. Microbiology. 2010 Sep;156(Pt 9):2621-9. doi: 10.1099/mic.0.040147-0. Epub 2010 Jun 3. PubMed PMID: 20522492; PubMed Central PMCID: PMC3068686.

12: Nakamura Y, Nakayama Y, Ando H, Tanaka A, Matsuo T, Okamoto S, Upham BL, Chang CC, Trosko JE, Park EY, Sato K. 3-Methylthiopropionic acid ethyl ester, isolated from Katsura-uri (Japanese pickling melon, Cucumis melo var. conomon), enhanced differentiation in human colon cancer cells. J Agric Food Chem. 2008 May 14;56(9):2977-84. doi: 10.1021/jf072898i. Epub 2008 Apr 22. PubMed PMID: 18426216; PubMed Central PMCID: PMC2435010.

13: Carrillo-Domínguez S, Carranco-Jauregui ME, Castillo-Domínguez RM, Castro-González MI, Avila-González E, Pérez-Gil F. Cholesterol and n-3 and n-6 fatty acid content in eggs from laying hens fed with red crab meal (Pleuroncodes planipes). Poult Sci. 2005 Jan;84(1):167-72. PubMed PMID: 15685957.

14: Okabe S, Arai T, Yamashita H, Sugihara K. Adenovirus-mediated prodrug-enzyme therapy for CEA-producing colorectal cancer cells. J Cancer Res Clin Oncol. 2003 Jun;129(6):367-73. Epub 2003 Jun 3. PubMed PMID: 12783317.

15: Soongswang J, Sangtawesin C, Sittiwangkul R, Wanitkun S, Muangmingsuk S, Sopontammarak S, Klungratana C, Kangkagate C. Myocardial diseases in Thai children. J Med Assoc Thai. 2002 Aug;85 Suppl 2:S648-57. PubMed PMID: 12403243.

16: van Wegen RJ, Lee SY, Middelberg AP. Metabolic and kinetic analysis of poly(3-hydroxybutyrate) production by recombinant Escherichia coli. Biotechnol Bioeng. 2001 Jul 5;74(1):70-80. PubMed PMID: 11353412.

17: Shimao Y, Nabeshima K, Inoue T, Koono M. TPA-enhanced motility and invasion in a highly metastatic variant (L-10) of human rectal adenocarcinoma cell line RCM-1: selective role of PKC-alpha and its inhibition by a combination of PDBu-induced PKC downregulation and antisense oligonucleotides treatment. Clin Exp Metastasis. 1999 Jun;17(4):351-60. PubMed PMID: 10545022.

18: Shimao Y, Nabeshima K, Inoue T, Koono M. Role of fibroblasts in HGF/SF-induced cohort migration of human colorectal carcinoma cells: fibroblasts stimulate migration associated with increased fibronectin production via upregulated TGF-beta1. Int J Cancer. 1999 Jul 30;82(3):449-58. PubMed PMID: 10399964.

19: Nabeshima K, Shimao Y, Inoue T, Itoh H, Kataoka H, Koono M. Hepatocyte growth factor/scatter factor induces not only scattering but also cohort migration of human colorectal-adenocarcinoma cells. Int J Cancer. 1998 Dec 9;78(6):750-9. PubMed PMID: 9833769.

20: Nabeshima K, Inoue T, Shimao Y, Kataoka H, Koono M. TPA-induced cohort migration of well-differentiated human rectal adenocarcinoma cells: cells move in a RGD-dependent manner on fibronectin produced by cells, and phosphorylation of E-cadherin/catenin complex is induced independently of cell-extracellular matrix interactions. Virchows Arch. 1998 Sep;433(3):243-53. PubMed PMID: 9769128.