UNC-669
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    WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 406494

CAS#: 314241-44-5

Description: UNC-669 is a L3MBTL domain inhibitor. UNC669 specifically targets lethal 3 malignant brain tumour-like protein (L3MBTL) with an IC50 of 5 μM.

Chemical Structure

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UNC-669
CAS# 314241-44-5
Product data Instruction

Theoretical Analysis

MedKoo Cat#: 406494
Name: UNC-669
CAS#: 314241-44-5
Chemical Formula: C15H20BrN3O
Exact Mass: 337.08
Molecular Weight: 338.243
Elemental Analysis: C, 53.26; H, 5.96; Br, 23.62; N, 12.42; O, 4.73

Price and Availability

Size Price Availability Quantity
10mg USD 150 Ready to ship
25mg USD 250 Ready to ship
50mg USD 350 Ready to ship
100mg USD 550 Ready to ship
200mg USD 750 Ready to ship
500mg USD 950 Ready to ship
1g USD 1450 Ready to ship
2g USD 2650 2 weeks
5g USD 3950 2 weeks
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Synonym: UNC669, UNC 669, UNC-669

IUPAC/Chemical Name: (5-bromopyridin-3-yl)(4-(pyrrolidin-1-yl)piperidin-1-yl)methanone

InChi Key: CQERVFFAOOUFEQ-UHFFFAOYSA-N

InChi Code: InChI=1S/C15H20BrN3O/c16-13-9-12(10-17-11-13)15(20)19-7-3-14(4-8-19)18-5-1-2-6-18/h9-11,14H,1-8H2

SMILES Code: O=C(C1=CC(Br)=CN=C1)N2CCC(N3CCCC3)CC2

Appearance: Pale orange-yellow solid

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO, not in water

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Biological target: UNC669 is a potent and selective MBT (malignant brain tumor) inhibitor with IC50 of 6 μM for L3MBTL1, 5- and 11-fold selective over L3MBTL3 and L3MBTL4.
In vitro activity: Inhibiting the MBT domain of PHF20L1 with small chemicals UNC1215 and UNC669 destabilized SOX2. PA-1 cells were incubated with UNC1215 or UNC669 for 24 h and then further treated with either DMSO or MG-132 for 4 h. Densitometry measurements illustrating the relative protein levels of SOX2 and PHF20L1, normalized to Cullin-1, were plotted (right panels). UNC1215 and UNC669 treatments did not affect the mRNA level of SOX2. Quantitative real-time PCR was performed to measure the mRNA level of SOX2 upon UNC1215 or UNC669 treatments. Results were normalized to β-actin. Treatment with UNC1215 or UNC669 shortened the half-life of SOX2 protein. PA-1 cells were incubated with 40 μM UNC1215, UNC669, or DMSO for 24 h, and followed by CHX treatment as indicated. The relative protein levels of SOX2 and PHF20L1 were densitometry quantified using Gel Image analysis software and plotted. The data were represented as mean ± SD Reference: Lab Invest. 2018 Dec;98(12):1627-1641. https://www.nature.com/articles/s41374-018-0106-8
In vivo activity: TBD

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 11.0 32.52
Ethanol 68.0 201.04

Preparing Stock Solutions

The following data is based on the product molecular weight 338.24 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol: 1. Wang Q, Yu M, Ma Y, Zhang X, Zhang H, Li S, Lan R, Lu F. PHF20L1 antagonizes SOX2 proteolysis triggered by the MLL1/WDR5 complexes. Lab Invest. 2018 Dec;98(12):1627-1641. doi: 10.1038/s41374-018-0106-8. Epub 2018 Aug 8. PMID: 30089852. 2. Zhou H, Che X, Bao G, Wang N, Peng L, Barnash KD, Frye SV, James LI, Bai X. Design, synthesis, and protein methyltransferase activity of a unique set of constrained amine containing compounds. Bioorg Med Chem Lett. 2016 Sep 15;26(18):4436-4440. doi: 10.1016/j.bmcl.2016.08.004. Epub 2016 Aug 3. PMID: 27528434; PMCID: PMC4996710.
In vitro protocol: 1. Wang Q, Yu M, Ma Y, Zhang X, Zhang H, Li S, Lan R, Lu F. PHF20L1 antagonizes SOX2 proteolysis triggered by the MLL1/WDR5 complexes. Lab Invest. 2018 Dec;98(12):1627-1641. doi: 10.1038/s41374-018-0106-8. Epub 2018 Aug 8. PMID: 30089852. 2. Zhou H, Che X, Bao G, Wang N, Peng L, Barnash KD, Frye SV, James LI, Bai X. Design, synthesis, and protein methyltransferase activity of a unique set of constrained amine containing compounds. Bioorg Med Chem Lett. 2016 Sep 15;26(18):4436-4440. doi: 10.1016/j.bmcl.2016.08.004. Epub 2016 Aug 3. PMID: 27528434; PMCID: PMC4996710.
In vivo protocol: TBD

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