WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 130105


Description: GBLI-2 serves as a bioluminescence probe for imaging of tumors, specifically granzyme B activity, during immunotherapy treatments. GBLI-2 consists of the mouse granzyme B tetrapeptide IEFD substrate that, through a self-immolative group, was conjugated to D-luciferin. For CT26 murine colorectal carcinoma in mice, GBLI-2 successfully correlated to the amount/level of granzyme B-expressing CD8+ T cells in tumors.

Chemical Structure


Theoretical Analysis

MedKoo Cat#: 130105
Name: GBLI-2
Chemical Formula: C45H51N7O12S2
Exact Mass: 945.3037
Molecular Weight: 946.06
Elemental Analysis: C, 57.13; H, 5.43; N, 10.36; O, 20.29; S, 6.78

Price and Availability

This product is not in stock, which may be available by custom synthesis. For cost-effective reason, minimum order is 1g (price is usually high, lead time is 2~3 months, depending on the technical challenge). Quote less than 1g will not be provided. To request quote, please email to sales or click below button.
Note: Price will be listed if it is available in the future.

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Synonym: GBLI-2; GBLI2; GBLI 2

IUPAC/Chemical Name: (S)-2-(6-((4-((2S,5S,8S,11R)-5-Benzyl-11-((R)-sec-butyl)-2,8-bis(2-carboxyethyl)-4,7,10,13-tetraoxo-3,6,9,12-tetraazatetradecanamido)benzyl)oxy)benzo[d]thiazol-2-yl)-4,5-dihydrothiazole-4-carboxylic acid


InChi Code: InChI=1S/C45H51N7O12S2/c1-4-24(2)38(46-25(3)53)42(61)49-32(17-19-37(56)57)40(59)50-33(20-26-8-6-5-7-9-26)41(60)48-31(16-18-36(54)55)39(58)47-28-12-10-27(11-13-28)22-64-29-14-15-30-35(21-29)66-44(51-30)43-52-34(23-65-43)45(62)63/h5-15,21,24,31-34,38H,4,16-20,22-23H2,1-3H3,(H,46,53)(H,47,58)(H,48,60)(H,49,61)(H,50,59)(H,54,55)(H,56,57)(H,62,63)/t24-,31+,32+,33+,34-,38-/m1/s1

SMILES Code: O=C([C@@H]1N=C(C2=NC3=CC=C(OCC4=CC=C(NC([C@H](CCC(O)=O)NC([C@H](CC5=CC=CC=C5)NC([C@H](CCC(O)=O)NC([C@@H]([C@H](C)CC)NC(C)=O)=O)=O)=O)=O)C=C4)C=C3S2)SC1)O

Appearance: To be determined

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: To be determined

Shelf Life: >2 years if stored properly

Drug Formulation: To be determined

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

Preparing Stock Solutions

The following data is based on the product molecular weight 946.06 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL

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1. Chen M, Zhou K, Dai SY, Tadepalli S, Balakrishnan PB, Xie J, Rami FEI, Dai T, Cui L, Idoyaga J, Rao J. In vivo bioluminescence imaging of granzyme B activity in tumor response to cancer immunotherapy. Cell Chem Biol. 2022 Oct 20;29(10):1556-1567.e6. doi: 10.1016/j.chembiol.2022.08.006. Epub 2022 Sep 13. PMID: 36103874; PMCID: PMC9588750.