MitoBlock-6
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MedKoo CAT#: 408184

CAS#: 303215-67-0

Description: MitoBlock-6 is an Augmenter of liver regeneration (ALR) inhibitor. MitoBloCK-6 attenuated the import of Erv1 substrates into yeast mitochondria and inhibited oxidation of Tim13 and Cmc1 in in vitro reconstitution assays. In addition, MitoBloCK-6 revealed an unexpected role for Erv1 in the carrier import pathway, namely transferring substrates from the translocase of the outer membrane complex onto the small Tim complexes. Cardiac development was impaired in MitoBloCK-6-exposed zebrafish embryos. Finally, MitoBloCK-6 induced apoptosis via cytochrome c release in human embryonic stem cells (hESCs) but not in differentiated cells, suggesting an important role for ALR in hESC homeostasis.


Chemical Structure

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MitoBlock-6
CAS# 303215-67-0

Theoretical Analysis

MedKoo Cat#: 408184
Name: MitoBlock-6
CAS#: 303215-67-0
Chemical Formula: C19H14Cl2N2O
Exact Mass: 356.05
Molecular Weight: 357.234
Elemental Analysis: C, 63.88; H, 3.95; Cl, 19.85; N, 7.84; O, 4.48

Price and Availability

Size Price Availability Quantity
100mg USD 450 2 Weeks
200mg USD 750 2 Weeks
500mg USD 1650 2 Weeks
1g USD 2950 2 Weeks
2g USD 5250 2 Weeks
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Synonym: MitoBlock-6; MitoBlock 6; MitoBlock6;

IUPAC/Chemical Name: 2-[[(4-Anilinophenyl)imino]methyl]-4,6-dichlorophenol

InChi Key: DBYOPSAQYAKIQV-WSDLNYQXSA-N

InChi Code: InChI=1S/C19H14Cl2N2O/c20-14-10-13(19(24)18(21)11-14)12-22-15-6-8-17(9-7-15)23-16-4-2-1-3-5-16/h1-12,23-24H/b22-12+

SMILES Code: OC1=C(Cl)C=C(Cl)C=C1/C=N/C2=CC=C(NC3=CC=CC=C3)C=C2

Appearance: To be determined

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: To be determined

Shelf Life: >2 years if stored properly

Drug Formulation: To be determined

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info: Augmenter of liver regeneration (ALR) is a critical multi-isoform protein with its longer isoform, located in the mitochondrial intermembrane space, being part of the mitochondrial disulfide relay system (DRS). Upregulation of ALR was observed in multiple forms of cancer, among them hepatocellular carcinoma (HCC).

Biological target:
In vitro activity:
In vivo activity:

Preparing Stock Solutions

The following data is based on the product molecular weight 357.23 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
In vitro protocol:
In vivo protocol:

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1: Kacprzak SM, Van Aken O. Carbon starvation, senescence and specific mitochondrial stresses, but not nitrogen starvation and general stresses, are major triggers for mitophagy in Arabidopsis. Autophagy. 2022 Dec;18(12):2894-2912. doi: 10.1080/15548627.2022.2054039. Epub 2022 Apr 3. PMID: 35311445; PMCID: PMC9673927.


2: Kabiri Y, Fuhrmann A, Becker A, Jedermann L, Eberhagen C, König AC, Silva TB, Borges F, Hauck SM, Michalke B, Knolle P, Zischka H. Mitochondrial Impairment by MitoBloCK-6 Inhibits Liver Cancer Cell Proliferation. Front Cell Dev Biol. 2021 Sep 20;9:725474. doi: 10.3389/fcell.2021.725474. PMID: 34616733; PMCID: PMC8488156.


3: Dabir DV, Hasson SA, Setoguchi K, Johnson ME, Wongkongkathep P, Douglas CJ, Zimmerman J, Damoiseaux R, Teitell MA, Koehler CM. A small molecule inhibitor of redox-regulated protein translocation into mitochondria. Dev Cell. 2013 Apr 15;25(1):81-92. doi: 10.1016/j.devcel.2013.03.006. PMID: 23597483; PMCID: PMC3726224.