WARNING: This product is for research use only, not for human or veterinary use.
MedKoo CAT#: 408184
Description: MitoBlock-6 is an Augmenter of liver regeneration (ALR) inhibitor. MitoBloCK-6 attenuated the import of Erv1 substrates into yeast mitochondria and inhibited oxidation of Tim13 and Cmc1 in in vitro reconstitution assays. In addition, MitoBloCK-6 revealed an unexpected role for Erv1 in the carrier import pathway, namely transferring substrates from the translocase of the outer membrane complex onto the small Tim complexes. Cardiac development was impaired in MitoBloCK-6-exposed zebrafish embryos. Finally, MitoBloCK-6 induced apoptosis via cytochrome c release in human embryonic stem cells (hESCs) but not in differentiated cells, suggesting an important role for ALR in hESC homeostasis.
MedKoo Cat#: 408184
Chemical Formula: C19H14Cl2N2O
Exact Mass: 356.0483
Molecular Weight: 357.234
Elemental Analysis: C, 63.88; H, 3.95; Cl, 19.85; N, 7.84; O, 4.48
Synonym: MitoBlock-6; MitoBlock 6; MitoBlock6;
IUPAC/Chemical Name: 2-[[(4-Anilinophenyl)imino]methyl]-4,6-dichlorophenol
InChi Key: DBYOPSAQYAKIQV-WSDLNYQXSA-N
InChi Code: InChI=1S/C19H14Cl2N2O/c20-14-10-13(19(24)18(21)11-14)12-22-15-6-8-17(9-7-15)23-16-4-2-1-3-5-16/h1-12,23-24H/b22-12+
SMILES Code: OC1=C(Cl)C=C(Cl)C=C1/C=N/C2=CC=C(NC3=CC=CC=C3)C=C2
Appearance: To be determined
Purity: >98% (or refer to the Certificate of Analysis)
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility: To be determined
Shelf Life: >2 years if stored properly
Drug Formulation: To be determined
Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code: 2934.99.9001
The following data is based on the product molecular weight 357.234 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||1.15 mL||5.76 mL||11.51 mL|
|5 mM||0.23 mL||1.15 mL||2.3 mL|
|10 mM||0.12 mL||0.58 mL||1.15 mL|
|50 mM||0.02 mL||0.12 mL||0.23 mL|
1: Kacprzak SM, Van Aken O. Carbon starvation, senescence and specific mitochondrial stresses, but not nitrogen starvation and general stresses, are major triggers for mitophagy in Arabidopsis. Autophagy. 2022 Dec;18(12):2894-2912. doi: 10.1080/15548627.2022.2054039. Epub 2022 Apr 3. PMID: 35311445; PMCID: PMC9673927.
2: Kabiri Y, Fuhrmann A, Becker A, Jedermann L, Eberhagen C, König AC, Silva TB, Borges F, Hauck SM, Michalke B, Knolle P, Zischka H. Mitochondrial Impairment by MitoBloCK-6 Inhibits Liver Cancer Cell Proliferation. Front Cell Dev Biol. 2021 Sep 20;9:725474. doi: 10.3389/fcell.2021.725474. PMID: 34616733; PMCID: PMC8488156.
3: Dabir DV, Hasson SA, Setoguchi K, Johnson ME, Wongkongkathep P, Douglas CJ, Zimmerman J, Damoiseaux R, Teitell MA, Koehler CM. A small molecule inhibitor of redox-regulated protein translocation into mitochondria. Dev Cell. 2013 Apr 15;25(1):81-92. doi: 10.1016/j.devcel.2013.03.006. PMID: 23597483; PMCID: PMC3726224.
Augmenter of liver regeneration (ALR) is a critical multi-isoform protein with its longer isoform, located in the mitochondrial intermembrane space, being part of the mitochondrial disulfide relay system (DRS). Upregulation of ALR was observed in multiple forms of cancer, among them hepatocellular carcinoma (HCC).