EDA-DA
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    WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 466255

CAS#: 87156-01-2

Description: EDA-DA is an unnatural dipeptide building block (an ethynyl-D-alanine and a D-alanine). It incorporates a biorthogonal alkyne group into peptidoglycan (PG) through MurF in the cytoplasmic pathway, which enables selective labeling via a click-chemistry reaction. EDA-DA allows labeling of PG in Gram-positive (B. subtilis), Gram-negative (E. coli and C. trachomatis), Mycobacterium (M. smegmatis) and moss plastids (P. patens) with azide modified fluorescent dyes such as Alexa Fluor 488.

Chemical Structure

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EDA-DA
CAS# 87156-01-2
Instruction

Theoretical Analysis

MedKoo Cat#: 466255
Name: EDA-DA
CAS#: 87156-01-2
Chemical Formula: C10H13F3N2O5
Exact Mass: 298.08
Molecular Weight: 298.218
Elemental Analysis: C, 40.28; H, 4.39; F, 19.11; N, 9.39; O, 26.82

Price and Availability

Size Price Availability Quantity
10mg USD 440 2 Weeks
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Synonym: EDA-DA; EDA DA; EDADA;

IUPAC/Chemical Name: ((R)-2-aminopent-4-ynoyl)-D-alanine compound with 2,2,2-trifluoroacetic acid (1:1)

InChi Key: BRIXKPONTJDKNO-KGZKBUQUSA-N

InChi Code: InChI=1S/C8H12N2O3.C2HF3O2/c1-3-4-6(9)7(11)10-5(2)8(12)13;3-2(4,5)1(6)7/h1,5-6H,4,9H2,2H3,(H,10,11)(H,12,13);(H,6,7)/t5-,6-;/m1./s1

SMILES Code: C[C@@H](NC([C@H](N)CC#C)=O)C(O)=O.FC(F)(C(O)=O)F

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: To be determined

Shelf Life: >2 years if stored properly

Drug Formulation: To be determined

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Biological target:
In vitro activity:
In vivo activity:

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 29.8 100.00
Water 29.8 100.00

Preparing Stock Solutions

The following data is based on the product molecular weight 298.22 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
In vitro protocol:
In vivo protocol:

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1: Hirano T, Tanidokoro K, Shimizu Y, Kawarabayasi Y, Ohshima T, Sato M, Tadano S, Ishikawa H, Takio S, Takechi K, Takano H. Moss Chloroplasts Are Surrounded by a Peptidoglycan Wall Containing D-Amino Acids. Plant Cell. 2016 Jul;28(7):1521-32. doi: 10.1105/tpc.16.00104. Epub 2016 Jun 20. PMID: 27325639; PMCID: PMC4981129.