WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 466235

CAS#: 69075-42-9

Description: 5-Ethynyluridine is a cell-permeable uridine analog. 5-Ethynyluridine can be used in vitro and in vivo to label newly synthesized RNA. 5-Ethynyluridine can be used to study changes in transcription.

Chemical Structure

CAS# 69075-42-9

Theoretical Analysis

MedKoo Cat#: 466235
Name: 5-Ethynyluridine
CAS#: 69075-42-9
Chemical Formula: C11H12N2O6
Exact Mass: 268.0695
Molecular Weight: 268.225
Elemental Analysis: C, 49.26; H, 4.51; N, 10.44; O, 35.79

Price and Availability

Size Price Availability Quantity
10.0mg USD 290.0 2 Weeks
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Synonym: 5-Ethynyluridine; 5Ethynyluridine; 5 Ethynyluridine;

IUPAC/Chemical Name: 1-((2R,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-ethynylpyrimidine-2,4(1H,3H)-dione


InChi Code: InChI=1S/C11H12N2O6/c1-2-5-3-13(11(18)12-9(5)17)10-8(16)7(15)6(4-14)19-10/h1,3,6-8,10,14-16H,4H2,(H,12,17,18)/t6-,7-,8-,10-/m1/s1

SMILES Code: OC[C@H]1O[C@H]([C@H](O)[C@@H]1O)N2C=C(C#C)C(NC2=O)=O

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: To be determined

Shelf Life: >2 years if stored properly

Drug Formulation: To be determined

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

Preparing Stock Solutions

The following data is based on the product molecular weight 268.225 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL

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7: Ierusalimsky VN, Balaban PM. Long-living RNA in the CNS of terrestrial snail. RNA Biol. 2018 Feb 1;15(2):207-213. doi: 10.1080/15476286.2017.1411460. Epub 2017 Dec 21. PMID: 29210316; PMCID: PMC5798958.

8: Palozola KC, Donahue G, Liu H, Grant GR, Becker JS, Cote A, Yu H, Raj A, Zaret KS. Mitotic transcription and waves of gene reactivation during mitotic exit. Science. 2017 Oct 6;358(6359):119-122. doi: 10.1126/science.aal4671. Epub 2017 Sep 14. PMID: 28912132; PMCID: PMC5727891.

9: Hida N, Aboukilila MY, Burow DA, Paul R, Greenberg MM, Fazio M, Beasley S, Spitale RC, Cleary MD. EC-tagging allows cell type-specific RNA analysis. Nucleic Acids Res. 2017 Sep 6;45(15):e138. doi: 10.1093/nar/gkx551. PMID: 28641402; PMCID: PMC5587779.

10: Liang Y, Suzol SH, Wen Z, Artiles AG, Mathivathanan L, Raptis RG, Wnuk SF. Uracil Nucleosides with Reactive Group at C5 Position: 5-(1-Halo-2-sulfonylvinyl)uridine Analogues. Org Lett. 2016 Mar 18;18(6):1418-21. doi: 10.1021/acs.orglett.6b00346. Epub 2016 Mar 2. PMID: 26933954.

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12: Yan F, Liu JJ, Ip V, Jamieson SM, McKeage MJ. Role of platinum DNA damage- induced transcriptional inhibition in chemotherapy-induced neuronal atrophy and peripheral neurotoxicity. J Neurochem. 2015 Dec;135(6):1099-112. doi: 10.1111/jnc.13355. Epub 2015 Oct 28. PMID: 26364854.

13: Kirschbaum M, Karimian G, Adelmeijer J, Giepmans BN, Porte RJ, Lisman T. Horizontal RNA transfer mediates platelet-induced hepatocyte proliferation. Blood. 2015 Aug 6;126(6):798-806. doi: 10.1182/blood-2014-09-600312. Epub 2015 Jun 8. PMID: 26056167