WARNING: This product is for research use only, not for human or veterinary use.
MedKoo CAT#: 556071
CAS#: Unknown
Description: 7C1 polymer is a novel ionazable lipid first reported by James E. Dahlman and Carmen Barnes et al. 7C1 polymer was made by the reaction of low-molecular-weight polyamine with lipid. 7C1 polymer can be positively charged, which efficiently binds siRNA to form nanoparticle. 7C1 polymer can deliver siRNA to endothelial cells with high efficiency, thereby facilitating the simultaneous silencing of multiple endothelial genes in vivo. Unlike lipid or lipid-like nanoparticles, this formulation does not significantly reduce gene expression in hepatocytes or immune cells even at the dosage necessary for endothelial gene silencing. These nanoparticles mediate the most durable non-liver silencing reported so far and facilitate the delivery of siRNAs that modify endothelial function in mouse models of vascular permeability, emphysema, primary tumour growth and metastasis.
MedKoo Cat#: 556071
Name: 7C1 polymer
CAS#: Unknown
Chemical Formula:
Exact Mass:
Molecular Weight:
Elemental Analysis:
Synonym: 7C1 polymer;
IUPAC/Chemical Name: poly(ethyleneamine) with C15 lipid.
InChi Key: N/A
InChi Code: N/A
SMILES Code: NCCN(CCNCC(O)CCCCCCCCCCCCC)CCNCCN(CCNCCN(CCN(CCNCCNCC(O)CCCCCCCCCCCCC)CCNCC(O)CCCCCCCCCCCCC)CCNCC(O)CCCCCCCCCCCCC)CCNCCNCC(O)CCCCCCCCCCCCC
Appearance: Solid powder
Purity: >98% (or refer to the Certificate of Analysis)
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility: To be determined
Shelf Life: >2 years if stored properly
Drug Formulation: To be determined
Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code: 2934.99.9001
| Biological target: | 7C1 is a low-molecular weight, ionizable polymer that forms nanoparticles. |
| In vitro activity: | TBD |
| In vivo activity: | To test the ability of 7C1 nanoparticles to achieve effective gene silencing in the endothelium, Tie2 was selected as the target gene because of its putative endothelial cell specificity. After confirming sequence homology of the Tie2 siRNA target site in mouse and nonhuman primate, the efficacy of the Tie2 siRNA formulated in 7C1 nanoparticles was first analyzed in a dose-response study in mice. he particle sizes measured by dynamic light scattering of the Tie2 and Luc control 7C1 siRNA nanoparticles were 86.8 ± 2.6 nm and 109.6 ± 2.6 nm, respectively. Reference: Sci Adv. 2018 Jun 27;4(6):eaar8409. https://pubmed.ncbi.nlm.nih.gov/29963629/ |
The following data is based on the product molecular weight Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
| Formulation protocol: | 1. Khan OF, Kowalski PS, Doloff JC, Tsosie JK, Bakthavatchalu V, Winn CB, Haupt J, Jamiel M, Langer R, Anderson DG. Endothelial siRNA delivery in nonhuman primates using ionizable low-molecular weight polymeric nanoparticles. Sci Adv. 2018 Jun 27;4(6):eaar8409. doi: 10.1126/sciadv.aar8409. PMID: 29963629; PMCID: PMC6021147. |
| In vitro protocol: | TBD |
| In vivo protocol: | 1. Khan OF, Kowalski PS, Doloff JC, Tsosie JK, Bakthavatchalu V, Winn CB, Haupt J, Jamiel M, Langer R, Anderson DG. Endothelial siRNA delivery in nonhuman primates using ionizable low-molecular weight polymeric nanoparticles. Sci Adv. 2018 Jun 27;4(6):eaar8409. doi: 10.1126/sciadv.aar8409. PMID: 29963629; PMCID: PMC6021147. |
Dahlman JE, Barnes C, Khan O, Thiriot A, Jhunjunwala S, Shaw TE, Xing Y, Sager HB, Sahay G, Speciner L, Bader A, Bogorad RL, Yin H, Racie T, Dong Y, Jiang S, Seedorf D, Dave A, Sandu KS, Webber MJ, Novobrantseva T, Ruda VM, Lytton-Jean AKR, Levins CG, Kalish B, Mudge DK, Perez M, Abezgauz L, Dutta P, Smith L, Charisse K, Kieran MW, Fitzgerald K, Nahrendorf M, Danino D, Tuder RM, von Andrian UH, Akinc A, Schroeder A, Panigrahy D, Kotelianski V, Langer R, Anderson DG. In vivo endothelial siRNA delivery using polymeric nanoparticles with low molecular weight. Nat Nanotechnol. 2014 Aug;9(8):648-655. doi: 10.1038/nnano.2014.84. Epub 2014 May 11. PMID: 24813696; PMCID: PMC4207430.
