ZZW-115
new
featured

    WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 555051

CAS#: 801991-87-7

Description: ZZW-115 is a potent NUPR1 inhibitor. ZZW-115 induces ferroptosis in a mitochondria-dependent manner. ZZW-115 induces ROS accumulation followed by a ferroptotic cell death, which could be prevented by ferrostatin-1 (Fer-1) and ROS-scavenging agents. In addition, ZZW-115-treatment increases the accumulation of hydroperoxided lipids in these cells. ZZW-115-induced mitochondrial morphological changes, compatible with the ferroptotic process, as well as mitochondrial network disorganization and strong mitochondrial metabolic dysfunction, which are rescued by both Fer-1 and N-acetylcysteine (NAC). Of note, the expression of TFAM, a key regulator of mitochondrial biogenesis, is downregulated by ZZW-115.


Chemical Structure

img
ZZW-115
CAS# 801991-87-7

Theoretical Analysis

MedKoo Cat#: 555051
Name: ZZW-115
CAS#: 801991-87-7
Chemical Formula: C24H31F3N4S
Exact Mass: 464.2222
Molecular Weight: 464.5952
Elemental Analysis: C, 62.05; H, 6.73; F, 12.27; N, 12.06; S, 6.90

Price and Availability

Size Price Availability Quantity
25.0mg USD 450.0 2 Weeks
50.0mg USD 750.0 2 Weeks
100.0mg USD 1250.0 2 Weeks
200.0mg USD 1950.0 2 Weeks
500.0mg USD 3250.0 2 Weeks
Bulk inquiry

Synonym: ZZW-115; ZZW 115; ZZW115;

IUPAC/Chemical Name: N,N-dimethyl-2-(4-(3-(2-(trifluoromethyl)-10H-phenothiazin-10-yl)propyl)piperazin-1-yl)ethan-1-amine

InChi Key: HUDONDPCYIGAMQ-UHFFFAOYSA-N

InChi Code: InChI=1S/C24H31F3N4S/c1-28(2)12-13-30-16-14-29(15-17-30)10-5-11-31-20-6-3-4-7-22(20)32-23-9-8-19(18-21(23)31)24(25,26)27/h3-4,6-9,18H,5,10-17H2,1-2H3

SMILES Code: FC(C(C=C1N2CCCN3CCN(CCN(C)C)CC3)=CC=C1SC4=C2C=CC=C4)(F)F

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: To be determined

Shelf Life: >2 years if stored properly

Drug Formulation: To be determined

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

Preparing Stock Solutions

The following data is based on the product molecular weight 464.5952 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

1: Rizzuti B, Lan W, Santofimia-Castaño P, Zhou Z, Velázquez-Campoy A, Abián O, Peng L, Neira JL, Xia Y, Iovanna JL. Design of Inhibitors of the Intrinsically Disordered Protein NUPR1: Balance between Drug Affinity and Target Function. Biomolecules. 2021 Oct 3;11(10):1453. doi: 10.3390/biom11101453. PMID: 34680086; PMCID: PMC8533202.

2: Huang C, Santofimia-Castaño P, Liu X, Xia Y, Peng L, Gotorbe C, Neira JL, Tang D, Pouyssegur J, Iovanna J. NUPR1 inhibitor ZZW-115 induces ferroptosis in a mitochondria-dependent manner. Cell Death Discov. 2021 Oct 1;7(1):269. doi: 10.1038/s41420-021-00662-2. PMID: 34599149; PMCID: PMC8486797.

3: Liu J, Song X, Kuang F, Zhang Q, Xie Y, Kang R, Kroemer G, Tang D. NUPR1 is a critical repressor of ferroptosis. Nat Commun. 2021 Jan 28;12(1):647. doi: 10.1038/s41467-021-20904-2. PMID: 33510144; PMCID: PMC7843652.

4: Santofimia-Castaño P, Lan W, Xia Y, Zhou Z, Huang C, Barea D, Cervello M, Giannitrapani L, Montalto G, Peng L, Iovanna J. Response to the Letter to the editor regarding "Targeting NUPR1 with the small compound ZZW-115 is an efficient strategy to treat hepatocellular carcinoma" by Jiong Lin. Cancer Lett. 2021 Mar 1;500:161-162. doi: 10.1016/j.canlet.2020.12.001. Epub 2020 Dec 5. PMID: 33290870.

5: Lin J. Letter to the editor regarding "Targeting NUPR1 with the small compound ZZW-115 is an efficient strategy to treat hepatocellular carcinoma". Cancer Lett. 2021 Feb 28;499:1. doi: 10.1016/j.canlet.2020.11.020. Epub 2020 Nov 21. PMID: 33232790.

6: Lan W, Santofimia-Castaño P, Swayden M, Xia Y, Zhou Z, Audebert S, Camoin L, Huang C, Peng L, Jiménez-Alesanco A, Velázquez-Campoy A, Abián O, Lomberk G, Urrutia R, Rizzuti B, Geli V, Soubeyran P, Neira JL, Iovanna J. ZZW-115-dependent inhibition of NUPR1 nuclear translocation sensitizes cancer cells to genotoxic agents. JCI Insight. 2020 Sep 17;5(18):e138117. doi: 10.1172/jci.insight.138117. PMID: 32780723; PMCID: PMC7526551.

7: Lan W, Santofimia-Castaño P, Xia Y, Zhou Z, Huang C, Fraunhoffer N, Barea D, Cervello M, Giannitrapani L, Montalto G, Peng L, Iovanna J. Targeting NUPR1 with the small compound ZZW-115 is an efficient strategy to treat hepatocellular carcinoma. Cancer Lett. 2020 Aug 28;486:8-17. doi: 10.1016/j.canlet.2020.04.024. Epub 2020 May 21. PMID: 32446862.

8: Santofimia-Castaño P, Xia Y, Peng L, Velázquez-Campoy A, Abián O, Lan W, Lomberk G, Urrutia R, Rizzuti B, Soubeyran P, Neira JL, Iovanna J. Targeting the Stress-Induced Protein NUPR1 to Treat Pancreatic Adenocarcinoma. Cells. 2019 Nov 17;8(11):1453. doi: 10.3390/cells8111453. PMID: 31744261; PMCID: PMC6912534.

9: Santofimia-Castaño P, Xia Y, Lan W, Zhou Z, Huang C, Peng L, Soubeyran P, Velázquez-Campoy A, Abián O, Rizzuti B, Neira JL, Iovanna J. Ligand-based design identifies a potent NUPR1 inhibitor exerting anticancer activity via necroptosis. J Clin Invest. 2019 Mar 28;129(6):2500-2513. doi: 10.1172/JCI127223. PMID: 30920390; PMCID: PMC6546470.

ZZW-115

25.0mg / USD 450.0