C12-200
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MedKoo CAT#: 556048

CAS#: 1220890-25-4

Description: C12-200 is potent branched-tail lipid. C12-200 is useful for the development of lipidoid nanoparticles for siRNA, mRNA, and vaccine Delivery. C12-200 formulation can increase the potency of erythropoietin-mRNA-loaded C12-200 lipid nanoparticles 7-fold relative to formulations previously used for siRNA delivery.

Chemical Structure

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C12-200
CAS# 1220890-25-4
Product data Instruction

Theoretical Analysis

MedKoo Cat#: 556048
Name: C12-200
CAS#: 1220890-25-4
Chemical Formula: C70H145N5O5
Exact Mass: 1,136.12
Molecular Weight: 1,136.960
Elemental Analysis: C, 73.95; H, 12.86; N, 6.16; O, 7.04

Price and Availability

Size Price Availability Quantity
10mg USD 150 Ready to Ship
25mg USD 250 Ready to Ship
50mg USD 450 Ready to Ship
100mg USD 750 Ready to Ship
200mg USD 1250 Ready to Ship
500mg USD 2650 Ready to Ship
1g USD 3850 Ready to Ship
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Synonym: C12-200; C 12-200; C-12-200; C12 200; C12200; C 12200; C-12200; Tech G 1;

IUPAC/Chemical Name: 1,1-((2-(4-(2-((2-(bis(2-hydroxydodecyl)amino)ethyl)(2-hydroxydodecyl) amino)ethyl)piperazin-1-yl)ethyl)azanediyl)bis(dodecan-2-ol)

InChi Key: URQSMTPJROAUPK-UHFFFAOYSA-N

InChi Code: InChI=1S/C70H145N5O5/c1-6-11-16-21-26-31-36-41-46-65(76)61-74(62-66(77)47-42-37-32-27-22-17-12-7-2)53-51-69(70(80)50-45-40-35-30-25-20-15-10-5)71-52-54-72-55-57-73(58-56-72)59-60-75(63-67(78)48-43-38-33-28-23-18-13-8-3)64-68(79)49-44-39-34-29-24-19-14-9-4/h65-71,76-80H,6-64H2,1-5H3

SMILES Code: CCCCCCCCCCC(O)C(NCCN1CCN(CCN(CC(O)CCCCCCCCCC)CC(O)CCCCCCCCCC)CC1)CCN(CC(O)CCCCCCCCCC)CC(O)CCCCCCCCCC

Appearance: Liquid

Purity: >90% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: To be determined

Shelf Life: >2 years if stored properly

Drug Formulation: To be determined

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Biological target: C12-200 is a benchmark ionizable cationic lipidoid along with helper lipids.
In vitro activity: The cationic lipidoid, C12-200, in the LNPs resulted in a net positive charge to the blank LNPs. This study prepared siRNA-LNPs using C12-200, a benchmark ionizable cationic lipidoid along with helper lipids. Reference: AAPS J. 2021 Dec 6;24(1):8. https://pubmed.ncbi.nlm.nih.gov/34873640/
In vivo activity: To test this theory, this study compared IV-injected lipid nanoparticles formulated with reporter mRNA incorporating five base modifications (ψ, m1ψ, m5U, m5C/ψ, and m5C/s2U) and four ionizable lipids (C12-200, cKK-E12, ZA3-Ep10, and 200Oi10) with tropism for different organs. C12-200 and cKK-E12 (also known as MD-1) are five- and four-tailed ionizable lipids that predominately deliver mRNA to the liver. The ionizable lipid 200Oi10, which has the same amino core as C12-200 but contains ester linkages next to the alkyl tails with a branch on the terminal carbon, induces protein expression in the spleen and the liver. Reference: J Control Release. 2022 Jan;341:206-214. https://pubmed.ncbi.nlm.nih.gov/34801660/

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 100.0 87.95
Ethanol 100.0 87.95

Preparing Stock Solutions

The following data is based on the product molecular weight 1,136.96 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol: 1. Khare P, Dave KM, Kamte YS, Manoharan MA, O'Donnell LA, Manickam DS. Development of Lipidoid Nanoparticles for siRNA Delivery to Neural Cells. AAPS J. 2021 Dec 6;24(1):8. doi: 10.1208/s12248-021-00653-2. PMID: 34873640; PMCID: PMC8648339. 2. Melamed JR, Hajj KA, Chaudhary N, Strelkova D, Arral ML, Pardi N, Alameh MG, Miller JB, Farbiak L, Siegwart DJ, Weissman D, Whitehead KA. Lipid nanoparticle chemistry determines how nucleoside base modifications alter mRNA delivery. J Control Release. 2022 Jan;341:206-214. doi: 10.1016/j.jconrel.2021.11.022. Epub 2021 Nov 18. PMID: 34801660; PMCID: PMC8905090. 3. Hajj KA, Melamed JR, Chaudhary N, Lamson NG, Ball RL, Yerneni SS, Whitehead KA. A Potent Branched-Tail Lipid Nanoparticle Enables Multiplexed mRNA Delivery and Gene Editing In Vivo. Nano Lett. 2020 Jul 8;20(7):5167-5175. doi: 10.1021/acs.nanolett.0c00596. Epub 2020 Jun 9. PMID: 32496069; PMCID: PMC7781386.
In vitro protocol: 1. Khare P, Dave KM, Kamte YS, Manoharan MA, O'Donnell LA, Manickam DS. Development of Lipidoid Nanoparticles for siRNA Delivery to Neural Cells. AAPS J. 2021 Dec 6;24(1):8. doi: 10.1208/s12248-021-00653-2. PMID: 34873640; PMCID: PMC8648339.
In vivo protocol: 1. Melamed JR, Hajj KA, Chaudhary N, Strelkova D, Arral ML, Pardi N, Alameh MG, Miller JB, Farbiak L, Siegwart DJ, Weissman D, Whitehead KA. Lipid nanoparticle chemistry determines how nucleoside base modifications alter mRNA delivery. J Control Release. 2022 Jan;341:206-214. doi: 10.1016/j.jconrel.2021.11.022. Epub 2021 Nov 18. PMID: 34801660; PMCID: PMC8905090. 2. Hajj KA, Melamed JR, Chaudhary N, Lamson NG, Ball RL, Yerneni SS, Whitehead KA. A Potent Branched-Tail Lipid Nanoparticle Enables Multiplexed mRNA Delivery and Gene Editing In Vivo. Nano Lett. 2020 Jul 8;20(7):5167-5175. doi: 10.1021/acs.nanolett.0c00596. Epub 2020 Jun 9. PMID: 32496069; PMCID: PMC7781386.

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