RWJ-54428
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MedKoo CAT#: 414251

CAS#: 189448-35-9

Description: RWJ-54428 is a new cephalosporin active against gram-positive bacteria, including methicillin-resistant Staphylococcus aureus (MRSA).


Chemical Structure

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RWJ-54428
CAS# 189448-35-9

Theoretical Analysis

MedKoo Cat#: 414251
Name: RWJ-54428
CAS#: 189448-35-9
Chemical Formula: C20H20ClN7O5S4
Exact Mass: 601.01
Molecular Weight: 602.110
Elemental Analysis: C, 39.90; H, 3.35; Cl, 5.89; N, 16.28; O, 13.29; S, 21.30

Price and Availability

Size Price Availability Quantity
1mg USD 150 3-4 weeks
5mg USD 550 3-4 weeks
10mg USD 950 3-4 weeks
25mg USD 1650 3-4 weeks
50mg USD 2950 3-4 weeks
100mg USD 5250 3-4 weeks
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Synonym: RWJ-54428; RWJ 54428; RWJ54428; MC02479; MC-02479; MC 02479

IUPAC/Chemical Name: 5-Thia-1-azabicyclo(4.2.0)oct-2-ene-2-carboxylic acid, 7-(((2-amino-5-chloro-4-thiazolyl)(hydroxyimino)acetyl)amino)-3-((3-(((2-aminoethyl)thio)methyl)-4-pyridinyl)thio)-8-oxo-, (6R-(6alpha,7beta(Z)))-

InChi Key: LTUWUNMGTLOPNC-RLQAYIIJSA-N

InChi Code: InChI=1S/C20H20ClN7O5S4/c21-15-11(26-20(23)37-15)12(27-33)16(29)25-13-17(30)28-14(19(31)32)10(7-35-18(13)28)36-9-1-3-24-5-8(9)6-34-4-2-22/h1,3,5,13,18,33H,2,4,6-7,22H2,(H2,23,26)(H,25,29)(H,31,32)/b27-12-/t13-,18-/m1/s1

SMILES Code: O=C(C(N12)=C(SC3=C(CSCCN)C=NC=C3)CS[C@]2([H])[C@H](NC(/C(C4=C(Cl)SC(N)=N4)=N\O)=O)C1=O)O

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: To be determined

Shelf Life: >2 years if stored properly

Drug Formulation: To be determined

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Biological target:
In vitro activity:
In vivo activity:

Preparing Stock Solutions

The following data is based on the product molecular weight 602.11 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
In vitro protocol:
In vivo protocol:

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1: Malouin F, Blais J, Chamberland S, Hoang M, Park C, Chan C, Mathias K, Hakem S, Dupree K, Liu E, Nguyen T, Dudley MN. RWJ-54428 (MC-02,479), a new cephalosporin with high affinity for penicillin-binding proteins, including PBP 2a, and stability to staphylococcal beta-lactamases. Antimicrob Agents Chemother. 2003 Feb;47(2):658-64. doi: 10.1128/AAC.47.2.658-664.2003. PMID: 12543674; PMCID: PMC151748.

2: Griffith DC, Harford L, Williams R, Lee VJ, Dudley MN. In vivo antibacterial activity of RWJ-54428, a new cephalosporin with activity against gram-positive bacteria. Antimicrob Agents Chemother. 2003 Jan;47(1):43-7. doi: 10.1128/AAC.47.1.43-47.2003. PMID: 12499167; PMCID: PMC149000.

3: Griffith DC, Rodriguez D, Corcoran E, Dudley MN. Pharmacodynamics of RWJ-54428 against Staphylococcus aureus, Streptococcus pneumoniae, and Enterococcus faecalis in a neutropenic mouse thigh infection model. Antimicrob Agents Chemother. 2008 Jan;52(1):244-7. doi: 10.1128/AAC.00776-07. Epub 2007 Oct 22. PMID: 17954697; PMCID: PMC2223871.

4: Chamberland S, Blais J, Hoang M, Dinh C, Cotter D, Bond E, Gannon C, Park C, Malouin F, Dudley MN. In vitro activities of RWJ-54428 (MC-02,479) against multiresistant gram-positive bacteria. Antimicrob Agents Chemother. 2001 May;45(5):1422-30. doi: 10.1128/AAC.45.5.1422-1430.2001. PMID: 11302805; PMCID: PMC90483.

5: Johnson AP, Warner M, Carter M, Livermore DM. In vitro activity of cephalosporin RWJ-54428 (MC-02479) against multidrug-resistant gram-positive cocci. Antimicrob Agents Chemother. 2002 Feb;46(2):321-6. doi: 10.1128/AAC.46.2.321-326.2002. PMID: 11796337; PMCID: PMC127043.

6: Hecker SJ, Glinka TW, Cho A, Zhang ZJ, Price ME, Chamberland S, Griffith D, Lee VJ. Discovery of RWJ-54428 (MC-02,479), a new cephalosporin active against resistant gram-positive bacteria. J Antibiot (Tokyo). 2000 Nov;53(11):1272-81. doi: 10.7164/antibiotics.53.1272. PMID: 11213288.

7: Swenson JM, Tenover FC. In vitro activity of a new cephalosporin, RWJ-54428, against streptococci, enterococci and staphylococci, including glycopeptide- intermediate Staphylococcus aureus. J Antimicrob Chemother. 2002 May;49(5):845-50. doi: 10.1093/jac/dkf020. PMID: 12003982.

8: Lee VJ, Hecker SJ. Antibiotic resistance versus small molecules, the chemical evolution. Med Res Rev. 1999 Nov;19(6):521-42. doi: 10.1002/(sici)1098-1128(199911)19:6<521::aid-med4>3.0.co;2-9. PMID: 10557368.

9: Hoffman-Roberts HL, C Babcock E, Mitropoulos IF. Investigational new drugs for the treatment of resistant pneumococcal infections. Expert Opin Investig Drugs. 2005 Aug;14(8):973-95. doi: 10.1517/13543784.14.8.973. PMID: 16050791.

10: Hoellman DB, Kelly LM, Jacobs MR, Appelbaum PC. In vitro anti-anaerobic activity of the cephalosporin derivative RWJ 54428, compared to seven other compounds. Clin Microbiol Infect. 2002 Dec;8(12):814-22. doi: 10.1046/j.1469-0691.2002.00475.x. PMID: 12519357.