WARNING: This product is for research use only, not for human or veterinary use.
MedKoo CAT#: 413853
CAS#: 3374-05-8
Description: Nalidixate sodium anhydrous is a synthetic 1,8-naphthyridine antimicrobial agent with a limited bacteriocidal spectrum. It is an inhibitor of the A subunit of bacterial DNA GYRASE.
MedKoo Cat#: 413853
Name: Nalidixate sodium anhydrous
CAS#: 3374-05-8
Chemical Formula: C12H11N2NaO3
Exact Mass: 254.0667
Molecular Weight: 254.2208
Elemental Analysis: C, 56.70; H, 4.36; N, 11.02; Na, 9.04; O, 18.88
Synonym: Nalidixate sodium anhydrous; Win18320; Win-18320; Win 18320
IUPAC/Chemical Name: 1,8-Naphthyridine-3-carboxylic acid, 1-ethyl-1,4-dihydro-7-methyl-4-oxo-, sodium salt
InChi Key: ROKRAUFZFDQWLE-UHFFFAOYSA-M
InChi Code: InChI=1S/C12H12N2O3.Na/c1-3-14-6-9(12(16)17)10(15)8-5-4-7(2)13-11(8)14;/h4-6H,3H2,1-2H3,(H,16,17);/q;+1/p-1
SMILES Code: O=C(C1=CN(CC)C2=NC(C)=CC=C2C1=O)[O-].[Na+]
Appearance: Solid powder
Purity: >98% (or refer to the Certificate of Analysis)
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility: To be determined
Shelf Life: >2 years if stored properly
Drug Formulation: To be determined
Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code: 2934.99.9001
| Biological target: | Nalidixic acid sodium salt, a quinolone antibiotic, is effective against both gram-positive and gram-negative bacteria. |
| In vitro activity: | Sodium nalidixate inhibited the cell growth and division of several respiratory competent strains of Saccharomyces cerevisiae. Reference: Mol Gen Genet. 1976 Jul 5;146(1):95-100. https://pubmed.ncbi.nlm.nih.gov/785214/ |
| In vivo activity: | Nalidixic acid preferentially suppressed growth in soft agar of transformed Balb/3T3 mouse cells induced by methylcholanthrene. Nalidixic acid suppressed growth in soft agar of NIH/3T3 mouse cells transformed by an activated c-Ha-ras, without affecting the amount of ras p21 proteins as detected by an immunoblotting analysis using a monoclonal antibody. Reference: Br J Cancer. 1989 Dec;60(6):880-6. https://pubmed.ncbi.nlm.nih.gov/2690912/ |
The following data is based on the product molecular weight 254.2208 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
| Formulation protocol: | 1. Chadha J, Khullar L. Subinhibitory concentrations of nalidixic acid alter bacterial physiology and induce anthropogenic resistance in a commensal strain of Escherichia coli in vitro. Lett Appl Microbiol. 2021 Nov;73(5):623-633. doi: 10.1111/lam.13550. Epub 2021 Aug 24. PMID: 34376018. 2. Carnevali F, Sarcoe LE, Whittaker PA. Differential effects of nalidixate on the cell growth of respiratory competent strains and cytoplasmic petite mutants of Saccharomyces cerevisiae. Mol Gen Genet. 1976 Jul 5;146(1):95-100. doi: 10.1007/BF00267988. PMID: 785214. 3. Kaneko M, Horikoshi J. Reversible suppression by nalidixic acid of anchorage-independent growth of mouse cells transformed by 3-methylcholanthrene or an activated c-Ha-ras gene. Br J Cancer. 1989 Dec;60(6):880-6. doi: 10.1038/bjc.1989.384. PMID: 2690912; PMCID: PMC2247252. 4. McQueen CA, Rosado RR, Williams GM. Effect of nalidixic acid on DNA repair in rat hepatocytes. Cell Biol Toxicol. 1989 Jun;5(2):201-6. doi: 10.1007/BF00122653. PMID: 2504447. |
| In vitro protocol: | 1. Chadha J, Khullar L. Subinhibitory concentrations of nalidixic acid alter bacterial physiology and induce anthropogenic resistance in a commensal strain of Escherichia coli in vitro. Lett Appl Microbiol. 2021 Nov;73(5):623-633. doi: 10.1111/lam.13550. Epub 2021 Aug 24. PMID: 34376018. 2. Carnevali F, Sarcoe LE, Whittaker PA. Differential effects of nalidixate on the cell growth of respiratory competent strains and cytoplasmic petite mutants of Saccharomyces cerevisiae. Mol Gen Genet. 1976 Jul 5;146(1):95-100. doi: 10.1007/BF00267988. PMID: 785214. |
| In vivo protocol: | 1. Kaneko M, Horikoshi J. Reversible suppression by nalidixic acid of anchorage-independent growth of mouse cells transformed by 3-methylcholanthrene or an activated c-Ha-ras gene. Br J Cancer. 1989 Dec;60(6):880-6. doi: 10.1038/bjc.1989.384. PMID: 2690912; PMCID: PMC2247252. 2. McQueen CA, Rosado RR, Williams GM. Effect of nalidixic acid on DNA repair in rat hepatocytes. Cell Biol Toxicol. 1989 Jun;5(2):201-6. doi: 10.1007/BF00122653. PMID: 2504447. |
1: Callao V, Ramos A. Actividad antibacteriana del ácido nalidíxico (Win 18320) frente a diversas estirpes de gram-negativos [Antibacterial activity of nalidixic acid (WIN 18320) on various strains of Gram-negative organisms]. Microbiol Esp. 1966 Apr-Jun;19(2):173-82. Spanish. PMID: 5974262.
2: TERRERI G, FORSTER RF. ESTUDO COMPARATIVO E OBSERVA C OES CL'INICAS DO WIN-18.320 ('ACIDO NALID'IXICO) EM INFEC C OES DO TRATO URIN'ARIO [COMPARATIVE STUDY AND CLINICAL OBSERVATIONS ON WIN-18320 (NALIDIXIC ACID) IN URINARY TRACT INFECTIONS]. Folha Med. 1964 May;48:283-7. Portuguese. PMID: 14271217.
