JF 526
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    WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 464513

CAS#: unknown

Description: JF 526 is a fluorogenic yellow fluorescent dye. It is supplied as an NHS ester for coupling to primary amine groups. NHS esters can be converted to relevant substrates for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. It is suitable for confocal fluorescent imaging and super-resolution microscopy (SRM) techniques, such as dSTORM (live and fixed cells) and STED. NHS esters can be multiplexed for two color imaging with Janelia Fluor® 635 SE. It is cell permeable and has an excitation maximum = 526 nm, emission maximum = 550 nm, quantum yield = 0.87, and extinction coefficient = 118,000 M-1cm-1.


Chemical Structure

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JF 526
CAS# unknown

Theoretical Analysis

MedKoo Cat#: 464513
Name: JF 526
CAS#: unknown
Chemical Formula: C31H19F6N3O7
Exact Mass: 659.1127
Molecular Weight: 659.4974
Elemental Analysis: C, 56.46; H, 2.90; F, 17.28; N, 6.37; O, 16.98

Price and Availability

Size Price Availability Quantity
1.0mg USD 480.0 2 Weeks
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Synonym: JF 526; JF526; JF-526; NHS; Janelia Fluor 526®, SE;

IUPAC/Chemical Name: 2-(3-(3,3-difluoroazetidin-1-ium-1-ylidene)-6-(3,3-difluoroazetidin-1-yl)-2,7-difluoro-3H-xanthen-9-yl)-4-(((2,5-dioxopyrrolidin-1-yl)oxy)carbonyl)benzoate

InChi Key: BCNDQOPFHGWJEL-UHFFFAOYSA-N

InChi Code: InChI=1S/C31H19F6N3O7/c32-19-6-17-23(8-21(19)38-10-30(34,35)11-38)46-24-9-22(39-12-31(36,37)13-39)20(33)7-18(24)27(17)16-5-14(1-2-15(16)28(43)44)29(45)47-40-25(41)3-4-26(40)42/h1-2,5-9H,3-4,10-13H2

SMILES Code: O=C(C1=CC=C(C(C(C2=CC(F)=C(C=C2OC3=C/C4=[N+]5CC(F)(C/5)F)N6CC(F)(C6)F)=C3C=C4F)=C1)C([O-])=O)ON7C(CCC7=O)=O

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: To be determined

Shelf Life: >2 years if stored properly

Drug Formulation: To be determined

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 13.19 20.0

Preparing Stock Solutions

The following data is based on the product molecular weight 659.4974 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL

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1: Zheng Q, Ayala AX, Chung I, Weigel AV, Ranjan A, Falco N, Grimm JB, Tkachuk AN, Wu C, Lippincott-Schwartz J, Singer RH, Lavis LD. Rational Design of Fluorogenic and Spontaneously Blinking Labels for Super-Resolution Imaging. ACS Cent Sci. 2019 Sep 25;5(9):1602-1613. doi: 10.1021/acscentsci.9b00676. Epub 2019 Sep 5. Erratum in: ACS Cent Sci. 2020 Oct 28;6(10):1844. PMID: 31572787; PMCID: PMC6764213.

JF 526

1.0mg / USD 480.0