JFN-75223 HCl

    WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 464503

CAS#: 70375-22-3 (hydrochloride)

Description: JFN-75223 is a fluorogenic substrate for trypsin, cathepsin B, and cathepsin H. Upon enzymatic cleavage by trypsin, cathepsin B, or cathepsin H, 7-amino-4-methylcoumarin (AMC) is released and its fluorescence can be used to quantify trypsin, cathepsin B, and cathepsin H activity. JFN-75223 displays excitation/emission maxima of 340-360/440-460 nm, respectively. This product has no formal name at the moment. For the convenience of communication, a temporary code name was therefore proposed according to MedKoo Chemical Nomenclature (see web page: https://www.medkoo.com/page/naming).

Chemical Structure

JFN-75223 HCl
CAS# 70375-22-3 (hydrochloride)

Theoretical Analysis

MedKoo Cat#: 464503
Name: JFN-75223 HCl
CAS#: 70375-22-3 (hydrochloride)
Chemical Formula: C24H28ClN5O5
Exact Mass:
Molecular Weight: 501.968
Elemental Analysis: C, 57.43; H, 5.62; Cl, 7.06; N, 13.95; O, 15.94

Price and Availability

Size Price Availability Quantity
10.0mg USD 240.0 2 Weeks
25.0mg USD 390.0 2 Weeks
50.0mg USD 630.0 2 Weeks
100.0mg USD 980.0 2 Weeks
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Synonym: JFN-75223 HCl; JFN75223 HCl; JFN 75223 HCl; Z-(L-Arg)-AMC (hydrochloride); Bz-Arg-AMC; N-benzoxy-L-Arg-7-amido-4-methylcoumarin; N-benzoyl-L-Arg-AMC; Z-Arg(HCl)AMC;

IUPAC/Chemical Name: benzyl (S)-(5-guanidino-1-((4-methyl-2-oxo-2H-chromen-7-yl)amino)-1-oxopentan-2-yl)carbamate hydrochloride


InChi Code: InChI=1S/C24H27N5O5.ClH/c1-15-12-21(30)34-20-13-17(9-10-18(15)20)28-22(31)19(8-5-11-27-23(25)26)29-24(32)33-14-16-6-3-2-4-7-16;/h2-4,6-7,9-10,12-13,19H,5,8,11,14H2,1H3,(H,28,31)(H,29,32)(H4,25,26,27);1H/t19-;/m0./s1


Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: To be determined

Shelf Life: >2 years if stored properly

Drug Formulation: To be determined

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
DMF 1.0 2.0
DMSO 1.0 2.0

Preparing Stock Solutions

The following data is based on the product molecular weight 501.968 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL

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1: Kramer J, Désor V, Brunst S, Wittmann SK, Lausen J, Heering J, Proschak A, Proschak E. A coupled fluorescence-based assay for the detection of protein arginine N-methyltransferase 6 (PRMT6) enzymatic activity. Anal Biochem. 2018 Apr 15;547:7-13. doi: 10.1016/j.ab.2018.01.023. Epub 2018 Feb 2. PMID: 29410016.

2: Smith AM, Dalton JP, Clough KA, Kilbane CL, Harrop SA, Hole N, Brindley PJ. Adult Schistosoma mansoni express cathepsin L proteinase activity. Mol Biochem Parasitol. 1994 Sep;67(1):11-9. doi: 10.1016/0166-6851(94)90091-4. PMID: 7838171.