HC Blue No. 1

    WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 464240

CAS#: 2784-94-3

Description: HC Blue No. 1 is a a semipermanent hair dye.

Chemical Structure

HC Blue No. 1
CAS# 2784-94-3

Theoretical Analysis

MedKoo Cat#: 464240
Name: HC Blue No. 1
CAS#: 2784-94-3
Chemical Formula: C11H17N3O4
Exact Mass: 255.1219
Molecular Weight: 255.274
Elemental Analysis: C, 51.76; H, 6.71; N, 16.46; O, 25.07

Price and Availability

This product is not in stock, which may be available by custom synthesis. For cost-effective reason, minimum order is 1g (price is usually high, lead time is 2~3 months, depending on the technical challenge). Quote less than 1g will not be provided. To request quote, please email to sales @medkoo.com or click below button.
Note: Price will be listed if it is available in the future.

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Synonym: HC Blue No. 1; HC Blue 1; HC Blue Number 1;

IUPAC/Chemical Name: 2,2'-((4-(methylamino)-3-nitrophenyl)azanediyl)bis(ethan-1-ol)


InChi Code: InChI=1S/C11H17N3O4/c1-12-10-3-2-9(8-11(10)14(17)18)13(4-6-15)5-7-16/h2-3,8,12,15-16H,4-7H2,1H3

SMILES Code: CNc1c([N+]([O-])=O)cc(N(CCO)CCO)cc1

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: To be determined

Shelf Life: >2 years if stored properly

Drug Formulation: To be determined

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

Preparing Stock Solutions

The following data is based on the product molecular weight 255.274 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL

Molarity Calculator

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1: HC Blue No. 1. IARC Monogr Eval Carcinog Risks Hum. 1993;57:129-42. PMID: 8207849; PMCID: PMC7682267.

2: Abu-Shakra A, Johnson L, Earley K, Jameson CW, Kari FW, Gupta R, Langenbach R. Isolation of the mutagenic and DNA adduct-inducing components from a commercial preparation of HC blue 1 using Salmonella (TA98) bioassay-directed HPLC fractionation. Mutat Res. 1991 Aug;260(4):377-85. doi: 10.1016/0165-1218(91)90023-f. PMID: 1870626.

3: Hill LE, Parton JW, Probst GS, Garriott ML. Mutagenicity evaluation of HC Blue No. 1 and HC Blue No. 2, II. Effect on the in vitro induction of unscheduled DNA synthesis in rat, mouse, rabbit, hamster, and monkey primary hepatocytes. Mutat Res. 1990 Jun;241(2):145-50. doi: 10.1016/0165-1218(90)90118-l. PMID: 2345554.

4: Parton JW, Beyers JE, Garriott ML. Mutagenicity evaluation of HC Blue No. 1 and HC Blue No. 2, I. Effect on the induction of micronuclei in mouse bone marrow cells. Mutat Res. 1990 Jun;241(2):139-44. doi: 10.1016/0165-1218(90)90117-k. PMID: 2345553.

5: Oberly TJ, Kokkino AJ, Bewsey BJ, Richardson KK. Mutagenicity evaluation of HC Blue No. 1 and HC Blue No. 2. III. Effects in the Salmonella typhimurium/Escherichia coli reversion assay and the mouse lymphoma L5178Y TK+/- forward mutation assay. Mutat Res. 1990 Jun;241(2):151-9. doi: 10.1016/0165-1218(90)90119-m. PMID: 2189067.

6: Kari FW, Driscoll SM, Abu-Shakra A, Strom SC, Jenkins WL, Volosin JS, Rudo KM, Langenbach R. Comparative metabolism and genotoxicity of the structurally similar nitrophenylenediamine dyes, HC Blue 1 and HC Blue 2, in mouse hepatocytes. Cell Biol Toxicol. 1990 Apr;6(2):139-55. doi: 10.1007/BF00249590. PMID: 2361189.

7: Kari F, Driscoll S, Parker C, Rudo K, Tomer K, Langenbach R. Species comparisons regarding comparative metabolism of two structurally similar phenylenediamines (HC Blue 1 and HC Blue 2). Prog Clin Biol Res. 1990;340D:305-14. PMID: 2371302.

8: Kari FW, Mennear JH, Farnell D, Thompson RB, Huff JE. Comparative carcinogenicity of two structurally similar phenylenediamine dyes (HC blue no. 1 and HC blue no. 2) in F344/N rats and B6C3F1 mice. Toxicology. 1989 Jun 1;56(2):155-65. doi: 10.1016/0300-483x(89)90130-3. PMID: 2734800.

9: National Toxicology Program. NTP Toxicology and Carcinogenesis Studies of C.I. Acid Orange 3 (CAS No. 6373-74-6) in F344/N Rats and B6C3F1 Mice (Gavage Studies). Natl Toxicol Program Tech Rep Ser. 1988 Dec;335:1-157. PMID: 12732901.

10: Burnett CM, Corbett JF. Failure of short-term in vitro mutagenicity tests to predict the animal carcinogenicity of hair dyes. Food Chem Toxicol. 1987 Sep;25(9):703-7. doi: 10.1016/0278-6915(87)90104-9. PMID: 3653824.

11: National Toxicology Program. NTP Toxicology and Carcinogenesis Studies of HC Blue No. 1 (CAS No. 2784-94-3) in F344/N Rats and B6C3F1 Mice (Feed Studies). Natl Toxicol Program Tech Rep Ser. 1985 Aug;271:1-192. PMID: 12748683.

12: Darroudi F, Sobels FH, Natarajan AT. Evaluation of the mutagenic potential of the hair dye N-methyl-amino-2-nitro-4-N',N'-bis-(2-hydroxyethyl)-aminobenzene in a battery consisting of Drosophila and mammalian cytogenetic assays. Mutat Res. 1983 Feb;116(2):169-78. doi: 10.1016/0165-1218(83)90108-8. PMID: 6402694.

13: Loprieno N, Mariani L, Rusciano D. Lack of genotoxic properties of the hair- dye component N-methyl-amino-2-nitro-4-N',N'-bis-(2-hydroxyethyl)-aminobenzene, in mammalian cells in vitro, and in yeasts. Mutat Res. 1983 Feb;116(2):161-8. doi: 10.1016/0165-1218(83)90107-6. PMID: 6402693.

14: Shahin MM, Bugaut A. Mutagenic evaluation of the monocyclic aromatic amine N-methylamino-2-nitro-4-N', N'-bis(2-hydroxyethyl)-aminobenzene in the Salmonella typhimurium/mammalian microsome test. Mutat Res. 1983 Feb;116(2):155-9. doi: 10.1016/0165-1218(83)90106-4. PMID: 6338359.