H-8208 trihydrochloride

    WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 576143

CAS#: 107882-63-3

Description: H-8208 trihydrochloride is a benzimidazole dye that has been shown to intercalate into DNA.


Chemical Structure

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H-8208 trihydrochloride
CAS# 107882-63-3

Theoretical Analysis

MedKoo Cat#: 576143
Name: H-8208 trihydrochloride
CAS#: 107882-63-3
Chemical Formula: C18H24Cl3N5
Exact Mass: 415.1097
Molecular Weight: 416.77
Elemental Analysis: C, 51.87; H, 5.80; Cl, 25.52; N, 16.80

Price and Availability

This product is not in stock, which may be available by custom synthesis. For cost-effective reason, minimum order is 1g (price is usually high, lead time is 2~3 months, depending on the technical challenge). Quote less than 1g will not be provided. To request quote, please email to sales @medkoo.com or click below button.
Note: Price will be listed if it is available in the future.

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Synonym: H-8208 trihydrochloride; H8208 trihydrochloride; H 8208 trihydrochloride; Hoechst 8208 trihydrochloride

IUPAC/Chemical Name: Benzenamine, 4-(5-(4-methyl-1-piperazinyl)-1H-benzimidazol-2-yl)-, trihydrochloride

InChi Key: PGVHDHFKFINXBN-UHFFFAOYSA-N

InChi Code: InChI=1S/C18H21N5.3ClH/c1-22-8-10-23(11-9-22)15-6-7-16-17(12-15)21-18(20-16)13-2-4-14(19)5-3-13;;;/h2-7,12H,8-11,19H2,1H3,(H,20,21);3*1H

SMILES Code: Cl.Cl.Cl.CN1CCN(CC1)c2ccc3[nH]c(nc3c2)c4ccc(N)cc4

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

Preparing Stock Solutions

The following data is based on the product molecular weight 416.77 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL

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1. Bardsley HJ, Sharples D, Grèzes JR. The binding of the benzimidazole dye H8208 to DNA and to polyinosinic-polycytidylic acid. J Pharm Pharmacol. 1987 Mar;39(3):212-4. doi: 10.1111/j.2042-7158.1987.tb06250.x. PMID: 2883286.

2. Hasegawa N, Kubota Y. Equilibrium and kinetic studies of the interaction of the benzimidazole dye Hoechst 8208 with nucleic acids. Nucleic Acids Symp Ser. 1993;(29):85-6. PMID: 8247805.

3. Nakamura H, Hifumi H, Kubota Y. The interaction of the benzimidazole dye Hoechst 8208 with nucleic acids. Nucleic Acids Symp Ser. 1990;(22):99-100. PMID: 2101927.