4-Nitrophenyl Palmitate

    WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 463373

CAS#: 1492-30-4

Description: 4-Nitrophenyl palmitate is a colorimetric lipase and esterase substrate. Upon enzymatic hydrolysis of 4-nitrophenyl palmitate, 4-nitrophenol is released which can be quantified by colorimetric detection at 410 nm as a measure of enzyme activity. 4-Nitrophenyl palmitate has been used to characterize the activity of various bacterial and mammalian enzymes, including Burkholderia and porcine pancreatic lipases.

Chemical Structure

4-Nitrophenyl Palmitate
CAS# 1492-30-4

Theoretical Analysis

MedKoo Cat#: 463373
Name: 4-Nitrophenyl Palmitate
CAS#: 1492-30-4
Chemical Formula: C22H35NO4
Exact Mass: 377.2566
Molecular Weight: 377.525
Elemental Analysis: C, 69.99; H, 9.35; N, 3.71; O, 16.95

Price and Availability

Size Price Availability Quantity
5.0g USD 550.0 2 Weeks
10.0g USD 950.0 2 Weeks
25.0g USD 1950.0 2 Weeks
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Synonym: 4-Nitrophenyl Palmitate; p-Nitrophenyl Palmitate; para-Nitrophenyl Palmitate; pNpp;

IUPAC/Chemical Name: 4-nitrophenyl palmitate


InChi Code: InChI=1S/C22H35NO4/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-22(24)27-21-18-16-20(17-19-21)23(25)26/h16-19H,2-15H2,1H3


Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >3 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

Preparing Stock Solutions

The following data is based on the product molecular weight 377.525 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL

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6: Takó M, KotogÁn A, Papp T, Kadaikunnan S, Alharbi NS, VÁgvölgyi C. Purification and Properties of Extracellular Lipases with Transesterification Activity and 1,3-Regioselectivity from Rhizomucor miehei and Rhizopus oryzae. J Microbiol Biotechnol. 2017 Feb 28;27(2):277-288. doi: 10.4014/jmb.1608.08005. PMID: 27780957.

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16: Ganasen P, Khan MR, Kalam MA, Mahmud MS. Effect of visible light on catalytic hydrolysis of p-nitrophenyl palmitate by the Pseudomonas cepacia lipase immobilized on sol-gel support. Bioprocess Biosyst Eng. 2014 Nov;37(11):2353-9. doi: 10.1007/s00449-014-1213-6. Epub 2014 May 31. PMID: 24879090.

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