BW284C51
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    WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 412166

CAS#: 402-40-4

Description: BW284C51 is a selective acetylcholinesterase inhibitor. It is also a nicotinic antagonist

Chemical Structure

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BW284C51
CAS# 402-40-4
Instruction

Theoretical Analysis

MedKoo Cat#: 412166
Name: BW284C51
CAS#: 402-40-4
Chemical Formula: C27H38Br2N2O
Exact Mass: 564.14
Molecular Weight: 566.420
Elemental Analysis: C, 57.25; H, 6.76; Br, 28.21; N, 4.95; O, 2.82

Price and Availability

Size Price Availability Quantity
25mg USD 350 2 Weeks
100mg USD 800 2 Weeks
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Synonym: BW 284C51; BW-284C51; BW284C51; BW284 C 51; BW-284 C 51; BW 284 C 51

IUPAC/Chemical Name: Benzenaminium, 4,4'-(3-oxo-1,5-pentanediyl)bis(N,N-dimethyl-N-2-propenyl-, dibromide

InChi Key: WKDURMTZOWGWTD-UHFFFAOYSA-L

InChi Code: InChI=1S/C27H38N2O.2BrH/c1-7-21-28(3,4)25-15-9-23(10-16-25)13-19-27(30)20-14-24-11-17-26(18-12-24)29(5,6)22-8-2;;/h7-12,15-18H,1-2,13-14,19-22H2,3-6H3;2*1H/q+2;;/p-2

SMILES Code: O=C(CCC1=CC=C([N+](C)(C)CC=C)C=C1)CCC2=CC=C([N+](C)(C)CC=C)C=C2.[Br-].[Br-]

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >3 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Biological target:
In vitro activity:
In vivo activity:

Preparing Stock Solutions

The following data is based on the product molecular weight 566.42 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
In vitro protocol:
In vivo protocol:

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1: Naik RS, Belinskaya T, Vinayaka CR, Saxena A. Monoclonal antibodies to fetal bovine serum acetylcholinesterase distinguish between acetylcholinesterases from ruminant and non-ruminant species. Chem Biol Interact. 2020 Oct 1;330:109225. doi: 10.1016/j.cbi.2020.109225. Epub 2020 Aug 12. PMID: 32795450.

2: Cao Y, Herrero-Nogareda L, Cedergreen N. A comparative study of acetylcholinesterase and general-esterase activity assays using different substrates, in vitro and in vivo exposures and model organisms. Ecotoxicol Environ Saf. 2020 Feb;189:109954. doi: 10.1016/j.ecoenv.2019.109954. Epub 2019 Nov 20. PMID: 31759743.

3: Mohamed MA, Shaalan S, Ghazy AM, Ali AA, Abd-Elaziz AM, Ghanem MME, Abd- Elghany SA. Purification and characterization of acetylcholinesterase in Rhynchophorus ferrugineus (Olivier) (Coleoptera: Curculionidae). Int J Biol Macromol. 2020 Mar 15;147:1029-1040. doi: 10.1016/j.ijbiomac.2019.10.071. Epub 2019 Nov 18. PMID: 31751747.

4: Casu V, Tardelli F, De Marchi L, Monni G, Cuccaro A, Oliva M, Freitas R, Pretti C. Soluble esterases as biomarkers of neurotoxic compounds in the widespread serpulid Ficopomatus enigmaticus (Fauvel, 1923). J Environ Sci Health B. 2019;54(11):883-891. doi: 10.1080/03601234.2019.1640028. Epub 2019 Jul 16. PMID: 31311415.

5: Gerlits O, Ho KY, Cheng X, Blumenthal D, Taylor P, Kovalevsky A, Radić Z. A new crystal form of human acetylcholinesterase for exploratory room-temperature crystallography studies. Chem Biol Interact. 2019 Aug 25;309:108698. doi: 10.1016/j.cbi.2019.06.011. Epub 2019 Jun 7. PMID: 31176713; PMCID: PMC6679772.

6: Xu ML, Luk WKW, Bi CWC, Liu EYL, Wu KQY, Yao P, Dong TTX, Tsim KWK. Erythropoietin regulates the expression of dimeric form of acetylcholinesterase during differentiation of erythroblast. J Neurochem. 2018 Aug;146(4):390-402. doi: 10.1111/jnc.14448. Epub 2018 Jul 23. PMID: 29675901.

7: Rivas-Hernández G, May-Uc Y, Noreña-Barroso E, Cobos-Gasca V, Rodríguez- Fuentes G. Plasma Cholinesterase Activity in Female Green Turtles Chelonia mydas Nesting in Laguna de Terminos, Mexico Related to Organochlorine Pesticides in Their Eggs. Bull Environ Contam Toxicol. 2018 Jan;100(1):101-105. doi: 10.1007/s00128-017-2250-z. Epub 2017 Dec 14. PMID: 29243208.

8: Meng X, Xu X, Bao H, Wang J, Liu Z. Characterization of the Fifth Putative Acetylcholinesterase in the Wolf Spider, Pardosa pseudoannulata. Molecules. 2017 Jul 11;22(7):1118. doi: 10.3390/molecules22071118. PMID: 28696352; PMCID: PMC6152279.

9: Spieker J, Mudersbach T, Vogel-Höpker A, Layer PG. Endochondral Ossification Is Accelerated in Cholinesterase-Deficient Mice and in Avian Mesenchymal Micromass Cultures. PLoS One. 2017 Jan 24;12(1):e0170252. doi: 10.1371/journal.pone.0170252. PMID: 28118357; PMCID: PMC5261733.

10: Hagstrom D, Hirokawa H, Zhang L, Radic Z, Taylor P, Collins ES. Planarian cholinesterase: in vitro characterization of an evolutionarily ancient enzyme to study organophosphorus pesticide toxicity and reactivation. Arch Toxicol. 2017 Aug;91(8):2837-2847. doi: 10.1007/s00204-016-1908-3. Epub 2016 Dec 18. PMID: 27990564; PMCID: PMC6485937.

11: Mennillo E, Casu V, Tardelli F, De Marchi L, Freitas R, Pretti C. Suitability of cholinesterase of polychaete Diopatra neapolitana as biomarker of exposure to pesticides: In vitro characterization. Comp Biochem Physiol C Toxicol Pharmacol. 2017 Jan;191:152-159. doi: 10.1016/j.cbpc.2016.10.007. Epub 2016 Oct 21. PMID: 27777085.

12: Rodríguez-Fuentes G, Marín-López V, Hernández-Márquez E. Cholinesterases in Gambusia yucatana: Biochemical Characterization and its Relationship with Sex and Total Length. Bull Environ Contam Toxicol. 2016 Dec;97(6):776-780. doi: 10.1007/s00128-016-1939-8. Epub 2016 Oct 11. PMID: 27730276.

13: Spieker J, Ackermann A, Salfelder A, Vogel-Höpker A, Layer PG. Acetylcholinesterase Regulates Skeletal In Ovo Development of Chicken Limbs by ACh-Dependent and -Independent Mechanisms. PLoS One. 2016 Aug 30;11(8):e0161675. doi: 10.1371/journal.pone.0161675. PMID: 27574787; PMCID: PMC5004892.

14: Estévez J, Selva V, Benabent M, Mangas I, Sogorb MÁ, Vilanova E. Acetylcholine-hydrolyzing activities in soluble brain fraction: Characterization with reversible and irreversible inhibitors. Chem Biol Interact. 2016 Nov 25;259(Pt B):374-381. doi: 10.1016/j.cbi.2016.08.004. Epub 2016 Aug 6. PMID: 27507601.

15: Meng X, Li C, Xiu C, Zhang J, Li J, Huang L, Zhang Y, Liu Z. Identification and Biochemical Properties of Two New Acetylcholinesterases in the Pond Wolf Spider (Pardosa pseudoannulata). PLoS One. 2016 Jun 23;11(6):e0158011. doi: 10.1371/journal.pone.0158011. PMID: 27337188; PMCID: PMC4919072.

16: Araújo MC, Assis CR, Silva LC, Machado DC, Silva KC, Lima AV, Carvalho LB Jr, Bezerra Rde S, Oliveira MB. Brain acetylcholinesterase of jaguar cichlid (Parachromis managuensis): From physicochemical and kinetic properties to its potential as biomarker of pesticides and metal ions. Aquat Toxicol. 2016 Aug;177:182-9. doi: 10.1016/j.aquatox.2016.05.019. Epub 2016 May 24. PMID: 27288599.

17: You H, Gobert GN, Du X, Pali G, Cai P, Jones MK, McManus DP. Functional characterisation of Schistosoma japonicum acetylcholinesterase. Parasit Vectors. 2016 Jun 10;9(1):328. doi: 10.1186/s13071-016-1615-1. PMID: 27283196; PMCID: PMC4901427.

18: Thangaraj G, Manakov V, Cucu A, Fournier C, Layer PG. Inflammatory effects of TNFα are counteracted by X-ray irradiation and AChE inhibition in mouse micromass cultures. Chem Biol Interact. 2016 Nov 25;259(Pt B):313-318. doi: 10.1016/j.cbi.2016.03.027. Epub 2016 Mar 24. PMID: 27019294.

19: Mohamed MA, Mahdy el-SM, Ghazy AE, Ibrahim NM, El-Mezayen HA, Ghanem MM. The activity of detoxifying enzymes in the infective juveniles of Heterorhabditis bacteriophora strains: Purification and characterization of two acetylcholinesterases. Comp Biochem Physiol C Toxicol Pharmacol. 2016 Feb;180:11-22. doi: 10.1016/j.cbpc.2015.10.014. Epub 2015 Nov 9. PMID: 26545490.

20: Almeida JR, Freitas M, Cruz S, Leão PN, Vasconcelos V, Cunha I. Acetylcholinesterase in Biofouling Species: Characterization and Mode of Action of Cyanobacteria-Derived Antifouling Agents. Toxins (Basel). 2015 Jul 24;7(8):2739-56. doi: 10.3390/toxins7082739. PMID: 26213967; PMCID: PMC4549721.