Pamidronic acid
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    WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 574839

CAS#: 40391-99-9

Description: Pamidronic acid is a biochemical.


Chemical Structure

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Pamidronic acid
CAS# 40391-99-9

Theoretical Analysis

MedKoo Cat#: 574839
Name: Pamidronic acid
CAS#: 40391-99-9
Chemical Formula: C3H11NO7P2
Exact Mass: 235.00
Molecular Weight: 235.069
Elemental Analysis: C, 15.33; H, 4.72; N, 5.96; O, 47.64; P, 26.35

Price and Availability

Size Price Availability Quantity
250mg USD 215 2 Weeks
1g USD 365 2 Weeks
5g USD 715 2 Weeks
Bulk inquiry

Synonym: Pamidronic acid

IUPAC/Chemical Name: (3-Amino-1-hydroxypropane-1,1-diyl)diphosphonic acid

InChi Key: WRUUGTRCQOWXEG-UHFFFAOYSA-N

InChi Code: InChI=1S/C3H11NO7P2/c4-2-1-3(5,12(6,7)8)13(9,10)11/h5H,1-2,4H2,(H2,6,7,8)(H2,9,10,11)

SMILES Code: OC(P(O)(O)=O)(P(O)(O)=O)CCN

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >3 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Product Data:
Biological target: Pamidronic acid is a drug used to treat a broad spectrum of bone absorption diseases.
In vitro activity: As expected, pamidronate disodium 1, 5, and 10 μg/ml were unfavorable for BMMSC growth (P < .05), whereas 0.1 and 0.5 μg/mL did not affect BMMSC growth (P ≥ .05). BMMSCs treated with pamidronate disodium 0.5 μg/mL had lower ALP activity, ALP staining, and ARS staining (P < .05), and BMMSCs treated with low concentrations (<0.5 μg/mL) of pamidronate disodium had the same levels of ALP activity, ALP staining, and ARS staining as the control (0 μg/mL). Pamidronate disodium 0.5 μg/mL decreased the expression of genes and proteins involved in Wnt and β-catenin signaling. Reference: J Oral Maxillofac Surg. 2017 Oct;75(10):2135-2143. https://pubmed.ncbi.nlm.nih.gov/28412267/
In vivo activity: Micro-CT and histology analyses indicated that PAM treatment for 2 or 10 weeks could completely prevent or reverse osteoarthritic subchondral bone loss and cartilage surface erosion. Immunohistochemistry and western blot analysis indicated that expression of OPG and RANKL increased, although RANKL expression increased more significantly than that of OPG. Therefore the ratio of OPG to RANKL was lower in the ACLT rabbit group. However, the ratio of OPG to RANKL in the PAM group was significantly higher than that in the ACLT group. Additionally, expression of MMP-9 and TLR-4 were upregulated in the ACLT group and downregulated in the PAM treated groups. Reference: BMC Musculoskelet Disord. 2014 Nov 6;15:370. https://pubmed.ncbi.nlm.nih.gov/25377946/

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 1.0 4.25
Water 30.5 129.75

Preparing Stock Solutions

The following data is based on the product molecular weight 235.07 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol: 1. Xu Y, Sun J, Yang X, Yu Y, Mai H, Li Z. Pamidronate Disodium Leads to Bone Necrosis via Suppression of Wnt/β-Catenin Signaling in Human Bone Marrow Mesenchymal Stem Cells In Vitro. J Oral Maxillofac Surg. 2017 Oct;75(10):2135-2143. doi: 10.1016/j.joms.2017.03.016. Epub 2017 Mar 22. PMID: 28412267. 2. Viereck V, Emons G, Lauck V, Frosch KH, Blaschke S, Gründker C, Hofbauer LC. Bisphosphonates pamidronate and zoledronic acid stimulate osteoprotegerin production by primary human osteoblasts. Biochem Biophys Res Commun. 2002 Mar 1;291(3):680-6. doi: 10.1006/bbrc.2002.6510. PMID: 11855844. 3. Lv Y, Xia JY, Chen JY, Zhao H, Yan HC, Yang HS, Li Q, Fan YX, Guo KJ, Chen XY. Effects of pamidronate disodium on the loss of osteoarthritic subchondral bone and the expression of cartilaginous and subchondral osteoprotegerin and RANKL in rabbits. BMC Musculoskelet Disord. 2014 Nov 6;15:370. doi: 10.1186/1471-2474-15-370. PMID: 25377946; PMCID: PMC4240862. 4. Kapitola J, Zák J. Effect of pamidronate on bone blood flow in oophorectomized rats. Physiol Res. 1998;47(4):237-40. PMID: 9803469.
In vitro protocol: 1. Xu Y, Sun J, Yang X, Yu Y, Mai H, Li Z. Pamidronate Disodium Leads to Bone Necrosis via Suppression of Wnt/β-Catenin Signaling in Human Bone Marrow Mesenchymal Stem Cells In Vitro. J Oral Maxillofac Surg. 2017 Oct;75(10):2135-2143. doi: 10.1016/j.joms.2017.03.016. Epub 2017 Mar 22. PMID: 28412267. 2. Viereck V, Emons G, Lauck V, Frosch KH, Blaschke S, Gründker C, Hofbauer LC. Bisphosphonates pamidronate and zoledronic acid stimulate osteoprotegerin production by primary human osteoblasts. Biochem Biophys Res Commun. 2002 Mar 1;291(3):680-6. doi: 10.1006/bbrc.2002.6510. PMID: 11855844.
In vivo protocol: 1. Lv Y, Xia JY, Chen JY, Zhao H, Yan HC, Yang HS, Li Q, Fan YX, Guo KJ, Chen XY. Effects of pamidronate disodium on the loss of osteoarthritic subchondral bone and the expression of cartilaginous and subchondral osteoprotegerin and RANKL in rabbits. BMC Musculoskelet Disord. 2014 Nov 6;15:370. doi: 10.1186/1471-2474-15-370. PMID: 25377946; PMCID: PMC4240862. 2. Kapitola J, Zák J. Effect of pamidronate on bone blood flow in oophorectomized rats. Physiol Res. 1998;47(4):237-40. PMID: 9803469.

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