RAMB4
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MedKoo CAT#: 408101

CAS#: 145888-79-5

Description: RAMB4, also known as PTP1B-IN-9, is a ubiquitin-proteasome system stressor. RAMB4 selectively reduces the viability of cervical cancer cells independently of HPV genotype via blockade of proteasomal degradation. RAMB4 treatment triggers a Ubiquitin-Proteasome-System-stress response without affecting 20S proteasome catalytic activities.

Chemical Structure

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RAMB4
CAS# 145888-79-5
Product data Instruction

Theoretical Analysis

MedKoo Cat#: 408101
Name: RAMB4
CAS#: 145888-79-5
Chemical Formula: C19H13Cl4NO
Exact Mass: 410.98
Molecular Weight: 413.120
Elemental Analysis: C, 55.24; H, 3.17; Cl, 34.32; N, 3.39; O, 3.87

Price and Availability

Size Price Availability Quantity
100mg USD 450 2 Weeks
200mg USD 750 2 Weeks
500mg USD 1250 2 Weeks
1g USD 1850 2 Weeks
2g USD 3250 2 Weeks
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Synonym: PTP1B-IN-9; PTP1B-IN 9; PTP1B-IN9; RAMB4; RAMB-4; RAMB 4;

IUPAC/Chemical Name: (3E,5E)-3,5-Bis(3,4-dichlorobenzylidene)piperidin-4-one

InChi Key: GJPXGFGIFQWUOC-ACFHMISVSA-N

InChi Code: InChI=1S/C19H13Cl4NO/c20-15-3-1-11(7-17(15)22)5-13-9-24-10-14(19(13)25)6-12-2-4-16(21)18(23)8-12/h1-8,24H,9-10H2/b13-5+,14-6+

SMILES Code: O=C1/C(CNC/C1=C\C2=CC=C(Cl)C(Cl)=C2)=C/C3=CC=C(Cl)C(Cl)=C3

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >3 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info: Cervical cancer cells exhibit an increased requirement for ubiquitin-dependent protein degradation associated with an elevated metabolic turnover rate, and for specific signaling pathways, notably HPV E6-targeted degradation of p53 and PDZ proteins. Natural compounds with antioxidant properties including flavonoids and triterpenoids hold promise as anticancer agents by interfering with ubiquitin-dependent protein degradation. An increasing body of evidence indicates that their α-β unsaturated carbonyl system is the molecular determinant for inhibition of ubiquitin-mediated protein degradation up-stream of the catalytic sites of the 20S proteasome.

Product Data:
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Biological target: RAMB4 is an inhibitor of the ubiquitin-proteasome system. It increases the levels of polyubiquitinated proteins in HeLa cervical cancer cells, but does not inhibit the chymotrypsin-like, trypsin-like, or peptidylglutamyl peptide hydrolyzing-like activities of the 20S proteasome.
In vitro activity: RAMB1 treatment results in activation of lysosomal-dependent degradation pathways as a mechanism to compensate for increasing levels of poly-ubiquitin enriched toxic aggregates. RAMB1 synergistically triggers cell death of cervical cancer cells when combined with Chloroquine. Reference: PLoS One. 2011;6(8):e23888. https://pubmed.ncbi.nlm.nih.gov/21909374/
In vivo activity: To be determined

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 1.0 2.42

Preparing Stock Solutions

The following data is based on the product molecular weight 413.12 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol: 1. Anchoori RK, Khan SR, Sueblinvong T, Felthauser A, Iizuka Y, Gavioli R, Destro F, Isaksson Vogel R, Peng S, Roden RB, Bazzaro M. Stressing the ubiquitin-proteasome system without 20S proteolytic inhibition selectively kills cervical cancer cells. PLoS One. 2011;6(8):e23888. doi: 10.1371/journal.pone.0023888. Epub 2011 Aug 31. PMID: 21909374; PMCID: PMC3166081.
In vitro protocol: 1. Anchoori RK, Khan SR, Sueblinvong T, Felthauser A, Iizuka Y, Gavioli R, Destro F, Isaksson Vogel R, Peng S, Roden RB, Bazzaro M. Stressing the ubiquitin-proteasome system without 20S proteolytic inhibition selectively kills cervical cancer cells. PLoS One. 2011;6(8):e23888. doi: 10.1371/journal.pone.0023888. Epub 2011 Aug 31. PMID: 21909374; PMCID: PMC3166081.
In vivo protocol: To be determined

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1 . Anchoori RK, et al. Stressing the ubiquitin-proteasome system without 20S proteolytic inhibition selectively kills cervical cancer cells.PLoS One. 2011;6(8):e23888.