MCU-i4
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MedKoo CAT#: 533794

CAS#: 371924-24-2

Description: MCU-i4 is a novel negative modulator of the MCU, binding MICU1 and impairing muscle cell growth.

Chemical Structure

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MCU-i4
CAS# 371924-24-2
Product data Instruction

Theoretical Analysis

MedKoo Cat#: 533794
Name: MCU-i4
CAS#: 371924-24-2
Chemical Formula: C23H27N3O2
Exact Mass: 377.21
Molecular Weight: 377.488
Elemental Analysis: C, 73.18; H, 7.21; N, 11.13; O, 8.48

Price and Availability

Size Price Availability Quantity
25mg USD 90 Ready to ship
50mg USD 150 Ready to ship
100mg USD 250 Ready to ship
200mg USD 450 Ready to ship
500mg USD 950 Ready to ship
1g USD 1650 Ready to ship
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Synonym: MCU-i4; MCUi4; MCU i4;

IUPAC/Chemical Name: Ethyl 4-((4-(diethylamino)phenyl)amino)-6-methylquinoline-3-carboxylate

InChi Key: RIWXBJCWHVMATR-UHFFFAOYSA-N

InChi Code: InChI=1S/C23H27N3O2/c1-5-26(6-2)18-11-9-17(10-12-18)25-22-19-14-16(4)8-13-21(19)24-15-20(22)23(27)28-7-3/h8-15H,5-7H2,1-4H3,(H,24,25)

SMILES Code: O=C(C1=C(NC2=CC=C(N(CC)CC)C=C2)C3=CC(C)=CC=C3N=C1)OCC

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >3 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Biological target: MCU-i4 blocks the IP3-dependent mitochondrial Ca2+-uptake, maintaining the gatekeeping role of their target.
In vitro activity: For this purpose, this study measured mitochondrial Ca2+ uptake speed in cells permeabilized with digitonin and then perfused with 3 μM Ca2+ in the presence of the active molecules (Figure 2C). MCU-i4 and MCU-i11 decreased mitochondrial Ca2+ uptake speed. These results confirm that the selected compounds impinge directly on the MCU complex activity rather than on Ca2+ release from intracellular stores or on cytosolic [Ca2+]. Reference: Cell Rep. 2020 Feb 18;30(7):2321-2331.e6. https://pubmed.ncbi.nlm.nih.gov/32075766/
In vivo activity: Both MCU-i4 and MCU-i11 inhibited mitochondrial Ca2+ uptake in myofibers (Figure 5C). Both compounds decreased myotube width (Figure 5D). These data prove the efficacy of MCU-i4 and MCU-i11 ex vivo and indicate that pharmacological modulation of mitochondrial Ca2+ uptake interferes with an essential biological process. Reference: Cell Rep. 2020 Feb 18;30(7):2321-2331.e6. https://pubmed.ncbi.nlm.nih.gov/32075766/

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 18.9 50.00
Ethanol 37.8 100.00

Preparing Stock Solutions

The following data is based on the product molecular weight 377.49 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol: 1. Di Marco G, Vallese F, Jourde B, Bergsdorf C, Sturlese M, De Mario A, Techer-Etienne V, Haasen D, Oberhauser B, Schleeger S, Minetti G, Moro S, Rizzuto R, De Stefani D, Fornaro M, Mammucari C. A High-Throughput Screening Identifies MICU1 Targeting Compounds. Cell Rep. 2020 Feb 18;30(7):2321-2331.e6. doi: 10.1016/j.celrep.2020.01.081. PMID: 32075766; PMCID: PMC7034061.
In vitro protocol: 1. Di Marco G, Vallese F, Jourde B, Bergsdorf C, Sturlese M, De Mario A, Techer-Etienne V, Haasen D, Oberhauser B, Schleeger S, Minetti G, Moro S, Rizzuto R, De Stefani D, Fornaro M, Mammucari C. A High-Throughput Screening Identifies MICU1 Targeting Compounds. Cell Rep. 2020 Feb 18;30(7):2321-2331.e6. doi: 10.1016/j.celrep.2020.01.081. PMID: 32075766; PMCID: PMC7034061.
In vivo protocol: 1. Di Marco G, Vallese F, Jourde B, Bergsdorf C, Sturlese M, De Mario A, Techer-Etienne V, Haasen D, Oberhauser B, Schleeger S, Minetti G, Moro S, Rizzuto R, De Stefani D, Fornaro M, Mammucari C. A High-Throughput Screening Identifies MICU1 Targeting Compounds. Cell Rep. 2020 Feb 18;30(7):2321-2331.e6. doi: 10.1016/j.celrep.2020.01.081. PMID: 32075766; PMCID: PMC7034061.

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1: Di Marco G, Vallese F, Jourde B, Bergsdorf C, Sturlese M, De Mario A, Techer- Etienne V, Haasen D, Oberhauser B, Schleeger S, Minetti G, Moro S, Rizzuto R, De Stefani D, Fornaro M, Mammucari C. A High-Throughput Screening Identifies MICU1 Targeting Compounds. Cell Rep. 2020 Feb 18;30(7):2321-2331.e6. doi: 10.1016/j.celrep.2020.01.081. PMID: 32075766; PMCID: PMC7034061.