p-APMSF
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MedKoo CAT#: 574257

CAS#: 74938-88-8

Description: p-APMSF is an irreversible inhibitor of serine proteases.


Chemical Structure

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p-APMSF
CAS# 74938-88-8

Theoretical Analysis

MedKoo Cat#: 574257
Name: p-APMSF
CAS#: 74938-88-8
Chemical Formula: C8H10ClFN2O2S
Exact Mass: 252.01
Molecular Weight: 252.688
Elemental Analysis: C, 38.03; H, 3.99; Cl, 14.03; F, 7.52; N, 11.09; O, 12.66; S, 12.69

Price and Availability

Size Price Availability Quantity
1mg USD 210
5mg USD 365
10mg USD 580
25mg USD 1000
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Synonym: p-APMSF, para-APMSF, (p-Amidinophenyl)methanesulfonyl Fluoride

IUPAC/Chemical Name: 4-(aminoiminomethyl)-benzenemethanesulfonyl fluoride, monohydrochloride

InChi Key: KHLLRHIUKOJXLL-UHFFFAOYSA-N

InChi Code: InChI=1S/C8H9FN2O2S.ClH/c9-14(12,13)5-6-1-3-7(4-2-6)8(10)11;/h1-4H,5H2,(H3,10,11);1H

SMILES Code: FS(CC1=CC=C(C(N)=N)C=C1)(=O)=O.Cl

Appearance: Solid powder

Purity: >95% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >3 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Product Data:
Biological target: p-APMSF is an irreversible inhibitor of serine proteases.
In vitro activity: the Ki of p-APMSF for all of the above-mentioned proteases is between 1 and 2 microM. In contrast, p-APMSF in large molar excess does not inactivate chymotrypsin or acetylcholinesterase. The unique reactivity of p-APMSF has been further shown in comparison with the related compound p-nitrophenyl (p-amidinophenyl)methanesulfonate which is an active-site titrant for thrombin but reacts poorly with Factor Xa, C1-r, and C1-s and is not hydrolyzed by bovine trypsin or human plasmin. Studies with bovine trypsin have demonstrated that the inhibitory activity of p-APMSF is the result of its interaction with the diisopropyl fluorophosphate reactive site. Reference: Biochemistry. 1980 Oct 14;19(21):4859-64. https://pubmed.ncbi.nlm.nih.gov/6775696/
In vivo activity: TBD

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 50.0 197.87
Water 25.0 98.94

Preparing Stock Solutions

The following data is based on the product molecular weight 252.69 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol: Laura R, Robison DJ, Bing DH. (p-Amidinophenyl)methanesulfonyl fluoride, an irreversible inhibitor of serine proteases. Biochemistry. 1980 Oct 14;19(21):4859-64. doi: 10.1021/bi00562a024. PMID: 6775696.
In vitro protocol: Laura R, Robison DJ, Bing DH. (p-Amidinophenyl)methanesulfonyl fluoride, an irreversible inhibitor of serine proteases. Biochemistry. 1980 Oct 14;19(21):4859-64. doi: 10.1021/bi00562a024. PMID: 6775696.
In vivo protocol: TBD

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1. Laura, R., Robison, D.J., and Bing, D.H. (p-Amidinophenyl)methanesulfonyl fluoride, an irreversible inhibitor of serine proteases. Biochemistry 19(21), 4859-4864 (1980).