WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 574167

CAS#: 30931-67-0

Description: ABTS is a radical cation and a peroxidase substrate. It is used to assess antioxidant capacity in the Trolox equivalent antioxidant capacity (TEAC) assay. It is blue in the presence of sodium persulfate or metmyoglobin but decolorizes upon incubation with antioxidants, and the antioxidant capacity can be determined spectrophotometrically. ABTS has also been used as an enzyme substrate in ELISAs. Upon incubation with a peroxidase, it produces a soluble green product that can be quantified by colorimetric detection at 405 nm.

Chemical Structure

CAS# 30931-67-0

Theoretical Analysis

MedKoo Cat#: 574167
Name: ABTS
CAS#: 30931-67-0
Chemical Formula: C18H24N6O6S4
Exact Mass: 548.064
Molecular Weight: 548.67
Elemental Analysis: C, 39.40; H, 4.41; N, 15.32; O, 17.50; S, 23.37

Price and Availability

Size Price Availability Quantity
1.0g USD 220.0 2 Weeks
5.0g USD 450.0 2 Weeks
10.0g USD 750.0 2 Weeks
25.0g USD 1475.0 2 Weeks
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Synonym: ABTS

IUPAC/Chemical Name: ammonium (2Z,2'Z)-2,2'-(hydrazine-1,2-diylidene)bis(3-ethyl-2,3-dihydrobenzo[d]thiazole-6-sulfonate)


InChi Code: InChI=1S/C18H18N4O6S4.2H3N/c1-3-21-13-7-5-11(31(23,24)25)9-15(13)29-17(21)19-20-18-22(4-2)14-8-6-12(32(26,27)28)10-16(14)30-18;;/h5-10H,3-4H2,1-2H3,(H,23,24,25)(H,26,27,28);2*1H3/b19-17-,20-18-;;

SMILES Code: [O-]S(C1=CC=C(N(CC)/C(S2)=N/N=C3SC(C=C(S([O-])(=O)=O)C=C4)=C4N\3CC)C2=C1)(=O)=O.[NH4+].[NH4+]

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >3 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

Preparing Stock Solutions

The following data is based on the product molecular weight 548.67 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL

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1. Bhattarai, H.D., Paudel, B., Lee, H.S., et al. Antioxidant activity of Sanionia uncinata, a polar moss species from King George Island, Antarctica. Phytother. Res. 22(12), 1635-1639 (2008).

2. Huang, D., Ou, B., and Prior, R.L. The chemistry behind antioxidant capacity assays. J. Agric. Food Chem. 53(6), 1841-1856 (2005).

3. Walker, R.B., and Everette, J.D. Comparative reaction rates of various antioxidants with ABTS radical cation. J. Agric. Food Chem. 57(4), 1156-1161 (2009).

4. Reyna-Bello, A., Eleizalde, M.C., and Silva, A.M. Assessment of chromogen suitability in ELISA for the detection of anaplasmosis and trypanosomosis. J. Immunoassay Immunochem. 28(1), 1-11 (2007).

5. Su, Y.C., and Wong, A.C. Detection of staphylococcal enterotoxin H by an enzyme-linked immunosorbent assay. J. Food Prot. 59(3), 327-330 (1996).


1.0g / USD 220.0