Danshensu sodium
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MedKoo CAT#: 574142

CAS#: 67920-52-9

Description: Danshensu sodium is a salvianolic acid that reduces expression of the autophagy-associated proteins p62, LC3-II, and Beclin-1 and the apoptosis-related proteins Bax and caspase-3, prevents cardiomyocyte damage. It increases heart rate, coronary flow (CF), and left ventricular developed pressure (LVDP). Danshensu reduces infarct size and improves left ventricular function in a rat model of myocardial infarction. It enhances radiation-induced tumor cell death in a Lewis lung carcinoma mouse xenograft model. Danshensu also decreases infarct volume, neuronal apoptosis, production of TNF-α, IL-1β, and IL-6, and superoxide dismutase (SOD) and glutathione peroxidase (GPx) activity in a rat model of cerebral ischemia and reperfusion injury.

Chemical Structure

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Danshensu sodium
CAS# 67920-52-9
Product data Instruction

Theoretical Analysis

MedKoo Cat#: 574142
Name: Danshensu sodium
CAS#: 67920-52-9
Chemical Formula: C9H9NaO5
Exact Mass: 220.03
Molecular Weight: 220.160
Elemental Analysis: C, 49.10; H, 4.12; Na, 10.44; O, 36.34

Price and Availability

Size Price Availability Quantity
100mg USD 300
1g USD 1410
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Synonym: Danshensu sodium

IUPAC/Chemical Name: α,3,4-trihydroxy-benzenepropanoic acid, monosodium salt

InChi Key: ZMMKVDBZTXUHFO-UHFFFAOYSA-M

InChi Code: InChI=1S/C9H10O5.Na/c10-6-2-1-5(3-7(6)11)4-8(12)9(13)14;/h1-3,8,10-12H,4H2,(H,13,14);/q;+1/p-1

SMILES Code: OC1=C(O)C=CC(CC(O)C([O-])=O)=C1.[Na+]

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >3 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Product Data:
Product Data
Instruction:
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Biological target: Danshensu (sodium salt) is sodium salt of danshensu from the widely used Chinese herb Danshen that can inhibit phenylephrine- and CaCl2-induced vasoconstriction in Ca2+-free medium
In vitro activity: The present study aimed at deciphering the effects of a bioactive phytochemical, sodium danshensu, on human oral cancer cell metastasis. he treatment of FaDu and Ca9-22 cells with different doses of sodium danshensu (25, 50, and 100 μM) caused a significant reduction in cellular motility, migration, and invasion, as compared to the untreated cells. This effect was associated with a reduced expression of MMP-2, vimentin and N-cadherin, together with an enhanced expression of E-cadherin and ZO-1. Further investigation on the molecular mechanism revealed that treatment with sodium danshensu caused significant reduction in p38 phosphorylation; however, phosphorylation of ERK1/2 significantly decreased only in FaDu cells, whereas p-JNK1/2 did not show any alteration. Collectively, the present study findings reveal that sodium danshensu execute anti-metastatic effect by suppressing p38 phosphorylation in human oral cancer Reference: Front Endocrinol (Lausanne). 2020 Sep 30;11:568436. https://pubmed.ncbi.nlm.nih.gov/33101201/
In vivo activity: In ischemic stroke mice, SDS ( sodium danshensu ) (700 mg/kg, i.p.) was injected 10 min after the onset of the ischemic insult. In the focal ischemic stroke model of the mouse, sham control and stroke animals received vehicle or SDS treatment (700 mg/kg, i.p., 10 min after ischemia and once daily) for 14 days. At 14 days after stroke and SDS or vehicle treatment, Western blot analysis showed that the SDS treatment enhanced the expression of VEGF, BDNF, SDF-1, and eNOS in the perfi-infarct region compared with vehicle controls (Fig. 1A to E). At 28 days after stroke, SDS treatment significantly increased the number of NeuN and BrdU co-labeled cells in the peri-infarct region compared with stroke vehicle controls (Figs. 3C and 3D). These results indicated that the SDS treatment promoted neurogenesis in the post-stroke brain. Reference: Cell Transplant. 2018 Apr; 27(4): 622–636. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7020234/

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 72.0 327.03
H2O 72.0 327.03

Preparing Stock Solutions

The following data is based on the product molecular weight 220.16 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol: 1. Kumar VB, Lin SH, Mahalakshmi B, Lo YS, Lin CC, Chuang YC, Hsieh MJ, Chen MK. Sodium Danshensu Inhibits Oral Cancer Cell Migration and Invasion by Modulating p38 Signaling Pathway. Front Endocrinol (Lausanne). 2020 Sep 30;11:568436. doi: 10.3389/fendo.2020.568436. PMID: 33101201; PMCID: PMC7554528. 2. Zhang N, Zou H, Jin L, Wang J, Zhong MF, Huang P, Gu BQ, Mao SL, Zhang C, Chen H. Biphasic effects of sodium danshensu on vessel function in isolated rat aorta. Acta Pharmacol Sin. 2010 Apr;31(4):421-8. doi: 10.1038/aps.2010.24. Epub 2010 Mar 15. PMID: 20228827; PMCID: PMC4007672. 3. Meng X, Jiang J, Pan H, Wu S, Wang S, Lou Y, Fan G. Preclinical Absorption, Distribution, Metabolism, and Excretion of Sodium Danshensu, One of the Main Water-Soluble Ingredients in Salvia miltiorrhiza, in Rats. Front Pharmacol. 2019 May 29;10:554. doi: 10.3389/fphar.2019.00554. PMID: 31231211; PMCID: PMC6558371. 4. Wei ZZ, Chen D, Liu LP, Gu X, Zhong W, Zhang YB, Wang Y, Yu SP, Wei L. Enhanced Neurogenesis and Collaterogenesis by Sodium Danshensu Treatment After Focal Cerebral Ischemia in Mice. Cell Transplant. 2018 Apr;27(4):622-636. doi: 10.1177/0963689718771889. PMID: 29984620; PMCID: PMC7020234.
In vitro protocol: 1. Kumar VB, Lin SH, Mahalakshmi B, Lo YS, Lin CC, Chuang YC, Hsieh MJ, Chen MK. Sodium Danshensu Inhibits Oral Cancer Cell Migration and Invasion by Modulating p38 Signaling Pathway. Front Endocrinol (Lausanne). 2020 Sep 30;11:568436. doi: 10.3389/fendo.2020.568436. PMID: 33101201; PMCID: PMC7554528. 2. Zhang N, Zou H, Jin L, Wang J, Zhong MF, Huang P, Gu BQ, Mao SL, Zhang C, Chen H. Biphasic effects of sodium danshensu on vessel function in isolated rat aorta. Acta Pharmacol Sin. 2010 Apr;31(4):421-8. doi: 10.1038/aps.2010.24. Epub 2010 Mar 15. PMID: 20228827; PMCID: PMC4007672.
In vivo protocol: 1. Meng X, Jiang J, Pan H, Wu S, Wang S, Lou Y, Fan G. Preclinical Absorption, Distribution, Metabolism, and Excretion of Sodium Danshensu, One of the Main Water-Soluble Ingredients in Salvia miltiorrhiza, in Rats. Front Pharmacol. 2019 May 29;10:554. doi: 10.3389/fphar.2019.00554. PMID: 31231211; PMCID: PMC6558371. 2. Wei ZZ, Chen D, Liu LP, Gu X, Zhong W, Zhang YB, Wang Y, Yu SP, Wei L. Enhanced Neurogenesis and Collaterogenesis by Sodium Danshensu Treatment After Focal Cerebral Ischemia in Mice. Cell Transplant. 2018 Apr;27(4):622-636. doi: 10.1177/0963689718771889. PMID: 29984620; PMCID: PMC7020234.

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1: Wang M, Tang W, Gong N, Liu P. Sodium Danshensu inhibits the progression of lung cancer by regulating PI3K/Akt signaling pathway. Drug Dev Res. 2022 Feb;83(1):88-96. doi: 10.1002/ddr.21846. Epub 2021 Jul 1. PMID: 34196024.


2: Gao Q, Deng H, Yang Z, Yang Q, Zhang Y, Yuan X, Zeng M, Guo M, Zeng W, Jiang X, Yu B. Sodium danshensu attenuates cerebral ischemia-reperfusion injury by targeting AKT1. Front Pharmacol. 2022 Sep 15;13:946668. doi: 10.3389/fphar.2022.946668. PMID: 36188542; PMCID: PMC9520076.


3: Hu S, Chen Y, Huang S, Liu M, Liu Y, Huang S. Sodium Danshensu protects against oxygen glucose deprivation/reoxygenation-induced astrocytes injury through regulating NOD-like receptor pyrin domain containing 3 (NLRP3) inflammasome and tuberous sclerosis complex-2 (TSC2)/mammalian target of rapamycin (mTOR) pathways. Ann Transl Med. 2022 Oct;10(20):1097. doi: 10.21037/atm-22-2143. PMID: 36388798; PMCID: PMC9652549.


4: Kumar VB, Lin SH, Mahalakshmi B, Lo YS, Lin CC, Chuang YC, Hsieh MJ, Chen MK. Sodium Danshensu Inhibits Oral Cancer Cell Migration and Invasion by Modulating p38 Signaling Pathway. Front Endocrinol (Lausanne). 2020 Sep 30;11:568436. doi: 10.3389/fendo.2020.568436. PMID: 33101201; PMCID: PMC7554528.


5: Zhang X, Yang Q, Zhang R, Zhang Y, Zeng W, Yu Q, Zeng M, Gan J, Li H, Yang L, Gao Q, Jiang X. Sodium Danshensu ameliorates cerebral ischemia/reperfusion injury by inhibiting CLIC4/NLRP3 inflammasome-mediated endothelial cell pyroptosis. Biofactors. 2023 Jul 17. doi: 10.1002/biof.1991. Epub ahead of print. PMID: 37458329.


6: Yang F, Shen C. Sodium Danshensu Cream Promotes the Healing of Pressure Ulcers in Mice through the Nrf2/HO-1 and NF-κB Pathways. Pharmaceuticals (Basel). 2022 Dec 13;15(12):1548. doi: 10.3390/ph15121548. PMID: 36558999; PMCID: PMC9783848.


7: Zeng M, Zhang X, Lv N, Wang L, Suo Y, Gan J, Yang L, Yu B, Jiang X, Zeng W. Sodium Danshensu stabilizes atherosclerotic vulnerable plaques by targeting IKKβ mediated inflammation in macrophages. Biomed Pharmacother. 2023 Sep;165:115153. doi: 10.1016/j.biopha.2023.115153. Epub 2023 Jul 10. PMID: 37437377.


8: Zhang QZ, Fu TT, Dai JN, Zhou ZN, Shen CZ. Sodium Danshensu promotes the healing of stage 2 pressure injury wounds in ischemia/reperfusion injury rat models: possible regulation of apoptosis and inflammatory response. J Tradit Chin Med. 2021 Aug;41(4):571-580. doi: 10.19852/j.cnki.jtcm.2021.03.009. PMID: 34392650.


9: Zhang N, Zou H, Jin L, Wang J, Zhong MF, Huang P, Gu BQ, Mao SL, Zhang C, Chen H. Biphasic effects of sodium danshensu on vessel function in isolated rat aorta. Acta Pharmacol Sin. 2010 Apr;31(4):421-8. doi: 10.1038/aps.2010.24. Epub 2010 Mar 15. PMID: 20228827; PMCID: PMC4007672.


10: Meng X, Jiang J, Pan H, Wu S, Wang S, Lou Y, Fan G. Preclinical Absorption, Distribution, Metabolism, and Excretion of Sodium Danshensu, One of the Main Water-Soluble Ingredients in Salvia miltiorrhiza, in Rats. Front Pharmacol. 2019 May 29;10:554. doi: 10.3389/fphar.2019.00554. PMID: 31231211; PMCID: PMC6558371.


11: Jiang J, Zhao X, Li X, Wu S, Yu S, Lou Y, Fan G. High-Throughput Determination of Sodium Danshensu in Beagle Dogs by the LCMS/MS Method, Employing Liquid-Liquid Extraction Based on 96-Well Format Plates. Molecules. 2017 Apr 25;22(5):667. doi: 10.3390/molecules22050667. PMID: 28441352; PMCID: PMC6154683.


12: Wu D, Xu J, Jiao W, Liu L, Yu J, Zhang M, Chen G. Suppression of Macrophage Activation by Sodium Danshensu via HIF-1α/STAT3/NLRP3 Pathway Ameliorated Collagen-Induced Arthritis in Mice. Molecules. 2023 Feb 6;28(4):1551. doi: 10.3390/molecules28041551. PMID: 36838542; PMCID: PMC9963181.


13: Wei ZZ, Chen D, Liu LP, Gu X, Zhong W, Zhang YB, Wang Y, Yu SP, Wei L. Enhanced Neurogenesis and Collaterogenesis by Sodium Danshensu Treatment After Focal Cerebral Ischemia in Mice. Cell Transplant. 2018 Apr;27(4):622-636. doi: 10.1177/0963689718771889. PMID: 29984620; PMCID: PMC7020234.


14: Wang C, Li H, Zhou K, Luo C, Li Y, Xie L, Hua Y. Sodium tanshinone IIA sulfonate and sodium danshensu open the placental barrier through down- regulation of placental P-glycoprotein in mice: implications in the transplacental digoxin treatment for fetal heart failure. Int J Cardiol. 2014 Oct 20;176(3):1331-3. doi: 10.1016/j.ijcard.2014.07.147. Epub 2014 Aug 3. PMID: 25127967.


15: Zhou L, Chow MS, Zuo Z. Effect of sodium caprate on the oral absorptions of danshensu and salvianolic acid B. Int J Pharm. 2009 Sep 8;379(1):109-18. doi: 10.1016/j.ijpharm.2009.06.016. Epub 2009 Jun 23. PMID: 19555749.


16: Wang J, Ma Z, Hong Z, Song J. [Tissue distribution in mice of danshensu from sodium danshensu and Salvia miltiorrhiza injection]. Zhongguo Zhong Yao Za Zhi. 2011 Jun;36(11):1516-8. Chinese. PMID: 22779190.


17: Guo C, Yin Y, Duan J, Zhu Y, Yan J, Wei G, Guan Y, Wu X, Wang Y, Xi M, Wen A. Neuroprotective effect and underlying mechanism of sodium danshensu [3-(3,4-dihydroxyphenyl) lactic acid from Radix and Rhizoma Salviae miltiorrhizae = Danshen] against cerebral ischemia and reperfusion injury in rats. Phytomedicine. 2015 Feb 15;22(2):283-9. doi: 10.1016/j.phymed.2014.12.001. Epub 2014 Dec 19. PMID: 25765834.


18: Alghamdi S, Baeissa HM, Azhar Kamal M, Rafeeq MM, Al Zahrani A, Maslum AA, Hakeem IJ, Alazragi RS, Alam Q. Unveiling the multitargeted potency of Sodium Danshensu against cervical cancer: a multitargeted docking-based, structural fingerprinting and molecular dynamics simulation study. J Biomol Struct Dyn. 2023 Aug 20:1-13. doi: 10.1080/07391102.2023.2248260. Epub ahead of print. PMID: 37599470.


19: Teng MC, Wu PC, Lin SP, Wu CY, Wang PH, Chen CT, Chen BY. Danshensu Decreases UVB-Induced Corneal Inflammation in an Experimental Mouse Model via Oral Administration. Curr Eye Res. 2018 Jan;43(1):27-34. doi: 10.1080/02713683.2017.1379543. Epub 2017 Nov 7. PMID: 29111819.


20: Qi HP, Wei SQ, Zhang LQ, Gao XC, Yu NN, Bi S, Cui H. Preventive effect of danshensu on selenite-induced cataractogenesis in cultured rat lens. Clin Exp Ophthalmol. 2013 Mar;41(2):172-9. doi: 10.1111/j.1442-9071.2012.02837.x. Epub 2012 Sep 4. PMID: 22712555.