WARNING: This product is for research use only, not for human or veterinary use.
MedKoo CAT#: 574121
Description: Dansyl chloride is a reactive probe for the derivatization of primary amines, including those on amino acids, peptides, and polyamines, for detection by HPLC. It has also been used for the derivatization of compounds containing phenol groups, such as steroids, for detection by MS/MS. Dansyl chloride is a fluorescent probe for proteins and enzymes. It displays excitation/emission maxima of 340/535 nm, respectively, in acetone.
MedKoo Cat#: 574121
Name: Dansyl chloride
Chemical Formula: C12H12ClNO2S
Exact Mass: 269.0277
Molecular Weight: 269.74
Elemental Analysis: C, 53.43; H, 4.48; Cl, 13.14; N, 5.19; O, 11.86; S, 11.89
Synonym: Dansyl chloride, DNS-Cl, NSC 83616
IUPAC/Chemical Name: 5-(dimethylamino)-1-naphthalenesulfonyl chloride
InChi Key: XPDXVDYUQZHFPV-UHFFFAOYSA-N
InChi Code: InChI=1S/C12H12ClNO2S/c1-14(2)11-7-3-6-10-9(11)5-4-8-12(10)17(13,15)16/h3-8H,1-2H3
SMILES Code: CN(C)C1=CC=CC2=C1C=CC=C2S(Cl)(=O)=O
Emission: 535 nm, in acetone
Excitation: 340 nm, in acetone
1. Walker, J.M. The dansyl method for identifying N-terminal amino acids. Methods in molecular biology: Basic protein and peptide protocols 32, 321-328 (1994).
2. Takeuchi, T. HPLC of amino acids as dansyl and dabsyl derivatives. J. Chromatogr. Lib. 70, 229-241 (2005).
3. Hunter, K.J. A dansyl chloride-HPLC method for the determination of polyamines. Methods in molecular biology: Polyamine protocols 79, 119-123 (1998).
4. Santa, T. Derivatization reagents in liquid chromatography/electrospray ionization tandem mass spectrometry. Biomed. Chromatogr. 25(1-2), 1-10 (2011).
5. Chen, R.F., and Scott, C.H. Atlas of fluorescence spectra and lifetimes of dyes attached to protein. Anal. Lett. 18(4), 393-421 (1985).