USP25/28-IN-AZ2

    WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 565719

CAS#: 2165322-95-0

Description: USP25/28-IN-AZ2 is a novel potent, selective, dual usp25/28 inhibitor

Chemical Structure

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USP25/28-IN-AZ2
CAS# 2165322-95-0
Instruction

Theoretical Analysis

MedKoo Cat#: 565719
Name: USP25/28-IN-AZ2
CAS#: 2165322-95-0
Chemical Formula: C17H17BrF3NO3
Exact Mass: 419.03
Molecular Weight: 420.226
Elemental Analysis: C, 48.59; H, 4.08; Br, 19.01; F, 13.56; N, 3.33; O, 11.42

Price and Availability

This product is not in stock, which may be available by custom synthesis. For cost-effective reason, minimum order is 1g (price is usually high, lead time is 2~3 months, depending on the technical challenge). Quote less than 1g will not be provided. To request quote, please email to sales @medkoo.com or click below button.
Note: Price will be listed if it is available in the future.

Synonym: USP25/28-IN-AZ2

IUPAC/Chemical Name: 2-((5-Bromo-2-((3-(trifluoromethoxy)benzyl)oxy)benzyl)amino)ethan-1-ol

InChi Key: FUYSDKCEPZFKSP-UHFFFAOYSA-N

InChi Code: InChI=1S/C17H17BrF3NO3/c18-14-4-5-16(13(9-14)10-22-6-7-23)24-11-12-2-1-3-15(8-12)25-17(19,20)21/h1-5,8-9,22-23H,6-7,10-11H2

SMILES Code: OCCNCC1=CC(Br)=CC=C1OCC2=CC=CC(OC(F)(F)F)=C2

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >3 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

Product Data:
Product Data
Instruction:
View handling instruction

Preparing Stock Solutions

The following data is based on the product molecular weight 420.226200000000000000000000000000 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL

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1: Wrigley JD, Gavory G, Simpson I, Preston M, Plant H, Bradley J, Goeppert AU, Rozycka E, Davies G, Walsh J, Valentine A, McClelland K, Odrzywol KE, Renshaw J, Boros J, Tart J, Leach L, Nowak T, Ward RA, Harrison T, Andrews DM. Identification and Characterization of Dual Inhibitors of the USP25/28 Deubiquitinating Enzyme Subfamily. ACS Chem Biol. 2017 Dec 15;12(12):3113-3125. doi: 10.1021/acschembio.7b00334. Epub 2017 Nov 28. PubMed PMID: 29131570. 2: Xu D, Liu J, Fu T, Shan B, Qian L, Pan L, Yuan J. USP25 regulates Wnt signaling by controlling the stability of tankyrases. Genes Dev. 2017 May 15;31(10):1024-1035. doi: 10.1101/gad.300889.117. Epub 2017 Jun 15. PubMed PMID: 28619731; PubMed Central PMCID: PMC5495119. 3: Long C, Lai Y, Li J, Huang J, Zou C. LPS promotes HBO1 stability via USP25 to modulate inflammatory gene transcription in THP-1 cells. Biochim Biophys Acta Gene Regul Mech. 2018 Sep;1861(9):773-782. doi: 10.1016/j.bbagrm.2018.08.001. Epub 2018 Aug 4. PubMed PMID: 30745998; PubMed Central PMCID: PMC6368258. 4: Ding J, Cheng Y, Zhang Y, Liao S, Yin T, Yang J. The miR-27a-3p/USP25 axis participates in the pathogenesis of recurrent miscarriage by inhibiting trophoblast migration and invasion. J Cell Physiol. 2019 Nov;234(11):19951-19963. doi: 10.1002/jcp.28593. Epub 2019 Apr 5. PubMed PMID: 30953360. 5: Zhong B, Liu X, Wang X, Chang SH, Liu X, Wang A, Reynolds JM, Dong C. Negative regulation of IL-17-mediated signaling and inflammation by the ubiquitin-specific protease USP25. Nat Immunol. 2012 Nov;13(11):1110-7. doi: 10.1038/ni.2427. Epub 2012 Oct 7. PubMed PMID: 23042150; PubMed Central PMCID: PMC3477275. 6: Valero R, Marfany G, González-Angulo O, González-González G, Puelles L, Gonzàlez-Duarte R. USP25, a novel gene encoding a deubiquitinating enzyme, is located in the gene-poor region 21q11.2. Genomics. 1999 Dec 15;62(3):395-405. PubMed PMID: 10644437. 7: Lin D, Zhang M, Zhang MX, Ren Y, Jin J, Zhao Q, Pan Z, Wu M, Shu HB, Dong C, Zhong B. Induction of USP25 by viral infection promotes innate antiviral responses by mediating the stabilization of TRAF3 and TRAF6. Proc Natl Acad Sci U S A. 2015 Sep 8;112(36):11324-9. doi: 10.1073/pnas.1509968112. Epub 2015 Aug 24. Erratum in: Proc Natl Acad Sci U S A. 2015 Oct 27;112(43):E5901. PubMed PMID: 26305951; PubMed Central PMCID: PMC4568686. 8: Qian G, Hu X, Li G, Ding Y, Zhu L, Zheng H, Li M, Li Z, Pan J, Li Y, Li G, Yang C, Liu Y, Xie Y, Lv H. Smurf1 restricts the antiviral function mediated by USP25 through promoting its ubiquitination and degradation. Biochem Biophys Res Commun. 2018 Apr 6;498(3):537-543. doi: 10.1016/j.bbrc.2018.03.015. Epub 2018 Mar 6. PubMed PMID: 29518389. 9: Yang Y, Shi L, Ding Y, Shi Y, Hu HY, Wen Y, Zhang N. Structural and Functional Investigations of the N-Terminal Ubiquitin Binding Region of Usp25. Biophys J. 2017 May 23;112(10):2099-2108. doi: 10.1016/j.bpj.2017.04.022. PubMed PMID: 28538147; PubMed Central PMCID: PMC5444020. 10: Ding C, Li F, Long Y, Zheng J. Chloroquine attenuates lipopolysaccharide-induced inflammatory responses through upregulation of USP25. Can J Physiol Pharmacol. 2017 May;95(5):481-491. doi: 10.1139/cjpp-2016-0303. Epub 2016 Nov 30. PubMed PMID: 28134560. 11: Kawaguchi K, Uo K, Tanaka T, Komada M. Tandem UIMs confer Lys48 ubiquitin chain substrate preference to deubiquitinase USP25. Sci Rep. 2017 Mar 22;7:45037. doi: 10.1038/srep45037. PubMed PMID: 28327663; PubMed Central PMCID: PMC5361193. 12: Liu B, Sureda-Gómez M, Zhen Y, Amador V, Reverter D. A quaternary tetramer assembly inhibits the deubiquitinating activity of USP25. Nat Commun. 2018 Nov 26;9(1):4973. doi: 10.1038/s41467-018-07510-5. PubMed PMID: 30478318; PubMed Central PMCID: PMC6255862. 13: Wen J, Bai H, Chen N, Zhang W, Zhu X, Li P, Gong J. USP25 promotes endotoxin tolerance via suppressing K48-linked ubiquitination and degradation of TRAF3 in Kupffer cells. Mol Immunol. 2019 Feb;106:53-62. doi: 10.1016/j.molimm.2018.12.017. Epub 2018 Dec 19. PubMed PMID: 30579117. 14: Ren Y, Zhao Y, Lin D, Xu X, Zhu Q, Yao J, Shu HB, Zhong B. The Type I Interferon-IRF7 Axis Mediates Transcriptional Expression of Usp25 Gene. J Biol Chem. 2016 Jun 17;291(25):13206-15. doi: 10.1074/jbc.M116.718080. Epub 2016 Apr 27. PubMed PMID: 27129230; PubMed Central PMCID: PMC4933234. 15: Sadler JBA, Lamb CA, Welburn CR, Adamson IS, Kioumourtzoglou D, Chi NW, Gould GW, Bryant NJ. The deubiquitinating enzyme USP25 binds tankyrase and regulates trafficking of the facilitative glucose transporter GLUT4 in adipocytes. Sci Rep. 2019 Mar 18;9(1):4710. doi: 10.1038/s41598-019-40596-5. PubMed PMID: 30886164; PubMed Central PMCID: PMC6423145. 16: Zhong H, Wang D, Fang L, Zhang H, Luo R, Shang M, Ouyang C, Ouyang H, Chen H, Xiao S. Ubiquitin-specific proteases 25 negatively regulates virus-induced type I interferon signaling. PLoS One. 2013 Nov 18;8(11):e80976. doi: 10.1371/journal.pone.0080976. eCollection 2013. PubMed PMID: 24260525; PubMed Central PMCID: PMC3832446. 17: Kim S, Lee D, Lee J, Song H, Kim HJ, Kim KT. Vaccinia-Related Kinase 2 Controls the Stability of the Eukaryotic Chaperonin TRiC/CCT by Inhibiting the Deubiquitinating Enzyme USP25. Mol Cell Biol. 2015 May;35(10):1754-62. doi: 10.1128/MCB.01325-14. Epub 2015 Mar 9. PubMed PMID: 25755282; PubMed Central PMCID: PMC4405641. 18: Masoumi KC, Marfany G, Wu Y, Massoumi R. Putative role of SUMOylation in controlling the activity of deubiquitinating enzymes in cancer. Future Oncol. 2016 Feb;12(4):565-74. doi: 10.2217/fon.15.320. Epub 2016 Jan 18. Review. PubMed PMID: 26777062. 19: Li J, Tan Q, Yan M, Liu L, Lin H, Zhao F, Bao G, Kong H, Ge C, Zhang F, Yu T, Li J, He X, Yao M. miRNA-200c inhibits invasion and metastasis of human non-small cell lung cancer by directly targeting ubiquitin specific peptidase 25. Mol Cancer. 2014 Jul 6;13:166. doi: 10.1186/1476-4598-13-166. PubMed PMID: 24997798; PubMed Central PMCID: PMC4105889. 20: Cheng H, Li X, Wang C, Chen Y, Li S, Tan J, Tan B, He Y. Inhibition of tankyrase by a novel small molecule significantly attenuates prostate cancer cell proliferation. Cancer Lett. 2019 Feb 28;443:80-90. doi: 10.1016/j.canlet.2018.11.013. Epub 2018 Nov 22. PubMed PMID: 30472184.