Nicotinamide Mononucleotide
featured

    WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 330188

CAS#: 1094-61-7

Description: Nicotinamide Mononucleotide, also known as ("NMN" and "β-NMN") is a nucleotide derived from ribose and nicotinamide Like nicotinamide riboside, NMN is a derivative of niacin, and humans have enzymes that can use NMN to generate nicotinamide adenine dinucleotide (NADH). Because NADH is a cofactor for processes inside mitochondria, for sirtuins, and for PARP, NMN has been studied in animal models as a potential neuroprotective and anti-aging agent. Dietary supplement companies have aggressively marketed NMN products claiming those benefits, though there is no clinical study on humans published yet.

Chemical Structure

img
Nicotinamide Mononucleotide
CAS# 1094-61-7
Product data Instruction

Theoretical Analysis

MedKoo Cat#: 330188
Name: Nicotinamide Mononucleotide
CAS#: 1094-61-7
Chemical Formula: C11H15N2O8P
Exact Mass: 334.06
Molecular Weight: 334.220
Elemental Analysis: C, 39.53; H, 4.52; N, 8.38; O, 38.30; P, 9.27

Price and Availability

Size Price Availability Quantity
5g USD 150 Ready to ship
10g USD 250 Ready to ship
25g USD 550 Ready to ship
50g USD 950 Ready to ship
Bulk inquiry

Synonym: Nicotinamide Mononucleotide; NMN; β-NMN; β-Nicotinamide mononucleotide;

IUPAC/Chemical Name: ((2R,3S,4R,5R)-5-(3-carbamoylpyridin-1-ium-1-yl)-3,4-dihydroxytetrahydrofuran-2-yl)methyl hydrogen phosphate

InChi Key: DAYLJWODMCOQEW-TURQNECASA-N

InChi Code: InChI=1S/C11H15N2O8P/c12-10(16)6-2-1-3-13(4-6)11-9(15)8(14)7(21-11)5-20-22(17,18)19/h1-4,7-9,11,14-15H,5H2,(H3-,12,16,17,18,19)/t7-,8-,9-,11-/m1/s1

SMILES Code: O=P(OC[C@H]1O[C@@H]([N+]2=CC=CC(C(N)=O)=C2)[C@H](O)[C@@H]1O)([O-])O

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >3 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Biological target: Human Endogenous Metabolite
In vitro activity: This particular molecule has demonstrated several beneficial pharmacological activities in preclinical studies, which suggest its potential therapeutic use. Mostly mediated by its involvement in NAD+ biosynthesis, the pharmacological activities of NMN include its role in cellular biochemical functions, cardioprotection, diabetes, Alzheimer's disease, and complications associated with obesity. The recent groundbreaking discovery of anti-ageing activities of this chemical moiety has added a valuable essence in the research involving this molecule. This review focuses on the biosynthesis of NMN in mammalian and prokaryotic cells and mechanism of absorption along with the reported pharmacological activities in murine model. Reference: Poddar SK, Sifat AE, Haque S, Nahid NA, Chowdhury S, Mehedi I. Nicotinamide Mononucleotide: Exploration of Diverse Therapeutic Applications of a Potential Molecule. Biomolecules. 2019 Jan 21;9(1):34. doi: 10.3390/biom9010034. PMID: 30669679; PMCID: PMC6359187.
In vivo activity: MN effectively mitigates age-associated physiological decline in mice. Without any obvious toxicity or deleterious effects, NMN suppressed age-associated body weight gain, enhanced energy metabolism, promoted physical activity, improved insulin sensitivity and plasma lipid profile, and ameliorated eye function and other pathophysiologies. Consistent with these phenotypes, NMN prevented age-associated gene expression changes in key metabolic organs and enhanced mitochondrial oxidative metabolism and mitonuclear protein imbalance in skeletal muscle. These effects of NMN highlight the preventive and therapeutic potential of NAD+ intermediates as effective anti-aging interventions in humans. Reference: Mills KF, Yoshida S, Stein LR, Grozio A, Kubota S, Sasaki Y, Redpath P, Migaud ME, Apte RS, Uchida K, Yoshino J, Imai SI. Long-Term Administration of Nicotinamide Mononucleotide Mitigates Age-Associated Physiological Decline in Mice. Cell Metab. 2016 Dec 13;24(6):795-806. doi: 10.1016/j.cmet.2016.09.013. Epub 2016 Oct 27. PMID: 28068222; PMCID: PMC5668137.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
PBS (pH 7.2) 10.0 29.92

Preparing Stock Solutions

The following data is based on the product molecular weight 334.22 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol: Poddar SK, Sifat AE, Haque S, Nahid NA, Chowdhury S, Mehedi I. Nicotinamide Mononucleotide: Exploration of Diverse Therapeutic Applications of a Potential Molecule. Biomolecules. 2019 Jan 21;9(1):34. doi: 10.3390/biom9010034. PMID: 30669679; PMCID: PMC6359187.
In vitro protocol: Poddar SK, Sifat AE, Haque S, Nahid NA, Chowdhury S, Mehedi I. Nicotinamide Mononucleotide: Exploration of Diverse Therapeutic Applications of a Potential Molecule. Biomolecules. 2019 Jan 21;9(1):34. doi: 10.3390/biom9010034. PMID: 30669679; PMCID: PMC6359187.
In vivo protocol: Mills KF, Yoshida S, Stein LR, Grozio A, Kubota S, Sasaki Y, Redpath P, Migaud ME, Apte RS, Uchida K, Yoshino J, Imai SI. Long-Term Administration of Nicotinamide Mononucleotide Mitigates Age-Associated Physiological Decline in Mice. Cell Metab. 2016 Dec 13;24(6):795-806. doi: 10.1016/j.cmet.2016.09.013. Epub 2016 Oct 27. PMID: 28068222; PMCID: PMC5668137.

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

1: Kiss T, Balasubramanian P, Valcarcel-Ares MN, Tarantini S, Yabluchanskiy A, Csipo T, Lipecz A, Reglodi D, Zhang XA, Bari F, Farkas E, Csiszar A, Ungvari Z. Nicotinamide mononucleotide (NMN) treatment attenuates oxidative stress and rescues angiogenic capacity in aged cerebromicrovascular endothelial cells: a potential mechanism for the prevention of vascular cognitive impairment. Geroscience. 2019 May 29. doi: 10.1007/s11357-019-00074-2. [Epub ahead of print] PubMed PMID: 31144244.

2: Lukacs M, Gilley J, Zhu Y, Orsomando G, Angeletti C, Liu J, Yang X, Park J, Hopkin RJ, Coleman MP, Zhai RG, Stottmann RW. Severe biallelic loss-of-function mutations in nicotinamide mononucleotide adenylyltransferase 2 (NMNAT2) in two fetuses with fetal akinesia deformation sequence. Exp Neurol. 2019 May 25:112961. doi: 10.1016/j.expneurol.2019.112961. [Epub ahead of print] PubMed PMID: 31136762.

3: Grozio A, Mills KF, Yoshino J, Bruzzone S, Sociali G, Tokizane K, Lei HC, Cunningham R, Sasaki Y, Migaud ME, Imai SI. Slc12a8 is a nicotinamide mononucleotide transporter. Nat Metab. 2019 Jan;1(1):47-57. doi: 10.1038/s42255-018-0009-4. Epub 2019 Jan 7. PubMed PMID: 31131364; PubMed Central PMCID: PMC6530925.

4: Tarantini S, Valcarcel-Ares MN, Toth P, Yabluchanskiy A, Tucsek Z, Kiss T, Hertelendy P, Kinter M, Ballabh P, Süle Z, Farkas E, Baur JA, Sinclair DA, Csiszar A, Ungvari Z. Nicotinamide mononucleotide (NMN) supplementation rescues cerebromicrovascular endothelial function and neurovascular coupling responses and improves cognitive function in aged mice. Redox Biol. 2019 Apr 10;24:101192. doi: 10.1016/j.redox.2019.101192. [Epub ahead of print] PubMed PMID: 31015147; PubMed Central PMCID: PMC6477631.

5: Song J, Li J, Yang F, Ning G, Zhen L, Wu L, Zheng Y, Zhang Q, Lin D, Xie C, Peng L. Nicotinamide mononucleotide promotes osteogenesis and reduces adipogenesis by regulating mesenchymal stromal cells via the SIRT1 pathway in aged bone marrow. Cell Death Dis. 2019 Apr 18;10(5):336. doi: 10.1038/s41419-019-1569-2. PubMed PMID: 31000692; PubMed Central PMCID: PMC6472410.

6: Bertoldo MJ, Uddin GM, Youngson NA, Agapiou D, Walters KA, Sinclair DA, Morris MJ, Gilchrist RB. Multigenerational obesity-induced perturbations in oocyte-secreted factor signalling can be ameliorated by exercise and nicotinamide mononucleotide. Hum Reprod Open. 2018 May 29;2018(3):hoy010. doi: 10.1093/hropen/hoy010. eCollection 2018. PubMed PMID: 30895251; PubMed Central PMCID: PMC6276682.

7: Hasegawa K. Novel tubular-glomerular interplay in diabetic kidney disease mediated by sirtuin 1, nicotinamide mononucleotide, and nicotinamide adenine dinucleotide Oshima Award Address 2017. Clin Exp Nephrol. 2019 Mar 11. doi: 10.1007/s10157-019-01719-4. [Epub ahead of print] Review. PubMed PMID: 30859351.

8: Klimova N, Long A, Kristian T. Nicotinamide mononucleotide alters mitochondrial dynamics by SIRT3-dependent mechanism in male mice. J Neurosci Res. 2019 Aug;97(8):975-990. doi: 10.1002/jnr.24397. Epub 2019 Feb 23. PubMed PMID: 30801823; PubMed Central PMCID: PMC6565489.

9: Poddar SK, Sifat AE, Haque S, Nahid NA, Chowdhury S, Mehedi I. Nicotinamide Mononucleotide: Exploration of Diverse Therapeutic Applications of a Potential Molecule. Biomolecules. 2019 Jan 21;9(1). pii: E34. doi: 10.3390/biom9010034. Review. PubMed PMID: 30669679; PubMed Central PMCID: PMC6359187.

10: Klimova N, Kristian T. Multi-targeted Effect of Nicotinamide Mononucleotide on Brain Bioenergetic Metabolism. Neurochem Res. 2019 Jan 19. doi: 10.1007/s11064-019-02729-0. [Epub ahead of print] PubMed PMID: 30661231.

11: Johnson S, Wozniak DF, Imai S. CA1 Nampt knockdown recapitulates hippocampal cognitive phenotypes in old mice which nicotinamide mononucleotide improves. NPJ Aging Mech Dis. 2018 Nov 8;4:10. doi: 10.1038/s41514-018-0029-z. eCollection 2018. PubMed PMID: 30416740; PubMed Central PMCID: PMC6224504.

12: Liang H, Gao J, Zhang C, Li C, Wang Q, Fan J, Wu Z, Wang Q. Nicotinamide mononucleotide alleviates Aluminum induced bone loss by inhibiting the TXNIP-NLRP3 inflammasome. Toxicol Appl Pharmacol. 2019 Jan 1;362:20-27. doi: 10.1016/j.taap.2018.10.006. Epub 2018 Oct 4. PubMed PMID: 30292833.

13: Pottorf T, Mann A, Fross S, Mansel C, Vohra BPS. Nicotinamide Mononucleotide Adenylyltransferase 2 maintains neuronal structural integrity through the maintenance of golgi structure. Neurochem Int. 2018 Dec;121:86-97. doi: 10.1016/j.neuint.2018.09.010. Epub 2018 Sep 29. PubMed PMID: 30278188.

14: Sims CA, Guan Y, Mukherjee S, Singh K, Botolin P, Davila A Jr, Baur JA. Nicotinamide mononucleotide preserves mitochondrial function and increases survival in hemorrhagic shock. JCI Insight. 2018 Sep 6;3(17). pii: 120182. doi: 10.1172/jci.insight.120182. eCollection 2018 Sep 6. PubMed PMID: 30185676; PubMed Central PMCID: PMC6171817.

15: Marinescu GC, Popescu RG, Stoian G, Dinischiotu A. β-nicotinamide mononucleotide (NMN) production in Escherichia coli. Sci Rep. 2018 Aug 16;8(1):12278. doi: 10.1038/s41598-018-30792-0. PubMed PMID: 30115969; PubMed Central PMCID: PMC6095924.

16: ERRATUM: Structural insights into Plasmodium falciparum nicotinamide mononucleotide adenylyltransferase: oligomeric assembly". Mem Inst Oswaldo Cruz. 2018;113(9):e180073ER. doi: 10.1590/0074-02760180073er. Epub 2018 Aug 9. PubMed PMID: 30110070; PubMed Central PMCID: PMC6088553.

17: Contreras-Rodríguez LE, Marin-Mogollon CY, Sánchez-Mejía LM, Ramírez-Hernández MH. Structural insights into Plasmodium falciparum nicotinamide mononucleotide adenylyltransferase: oligomeric assembly. Mem Inst Oswaldo Cruz. 2018 Jul 10;113(9):e180073. doi: 10.1590/0074-02760180073. Erratum in: Mem Inst Oswaldo Cruz. 2018;113(9):e180073ER. PubMed PMID: 29995110; PubMed Central PMCID: PMC6037046.

18: Nadtochiy SM, Wang YT, Nehrke K, Munger J, Brookes PS. Cardioprotection by nicotinamide mononucleotide (NMN): Involvement of glycolysis and acidic pH. J Mol Cell Cardiol. 2018 Aug;121:155-162. doi: 10.1016/j.yjmcc.2018.06.007. Epub 2018 Jun 26. PubMed PMID: 29958828; PubMed Central PMCID: PMC6103815.

19: Marinescu GC, Popescu RG, Dinischiotu A. Size Exclusion Chromatography Method for Purification of Nicotinamide Mononucleotide (NMN) from Bacterial Cells. Sci Rep. 2018 Mar 13;8(1):4433. doi: 10.1038/s41598-018-22806-8. PubMed PMID: 29535407; PubMed Central PMCID: PMC5849608.

20: Konishi K, Ueda S, Kawano M, Osawa S, Tamura T, Hokazono E, Kayamori Y, Sakasegawa SI. Characterization and application of a novel nicotinamide mononucleotide adenylyltransferase from Thermus thermophilus HB8. J Biosci Bioeng. 2018 Apr;125(4):385-389. doi: 10.1016/j.jbiosc.2017.10.017. Epub 2017 Nov 27. PubMed PMID: 29175123.