BN-82002
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MedKoo CAT#: 555387

CAS#: 396073-89-5

Description: BN-82002, also known as CDC25 Phosphatase Inhibitor I, is a cell-permeable ortho-hydroxybenzylamino compound that displays anti-tumor properties. BN-82002 acts as a potent, selective, and irreversible inhibitor of CDC25 phosphatase family (IC50 = 2.4, 3.9, 6.3, 5.4, and 4.6 µM for 25A, 25B2, 25B3, 25C, and 25C-cat, respectively). BN82002 inhibits the phosphatase activity of recombinant human CDC25A, B, and C in vitro. It impairs the proliferation of tumoral cell lines and increases cyclin-dependent kinase 1 inhibitory tyrosine phosphorylation. In synchronized HeLa cells, BN82002 delays cell cycle progression at G1-S, in S phase and at the G2-M transition.

Chemical Structure

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BN-82002
CAS# 396073-89-5
Product data Instruction

Theoretical Analysis

MedKoo Cat#: 555387
Name: BN-82002
CAS#: 396073-89-5
Chemical Formula: C19H25N3O4
Exact Mass: 359.18
Molecular Weight: 359.426
Elemental Analysis: C, 63.49; H, 7.01; N, 11.69; O, 17.81

Price and Availability

Size Price Availability Quantity
50mg USD 350 2 weeks
100mg USD 550 2 weeks
200mg USD 850 2 weeks
500mg USD 1650 2 weeks
1g USD 2450 2 weeks
2g USD 3950 2 weeks
Bulk inquiry

Synonym: BN-82002; BN 82002; BN82002; CDC25 Phosphatase Inhibitor I; PTP Inhibitor XX;

IUPAC/Chemical Name: Phenol, 4-(dimethylamino)-2-methoxy-6-((methyl(2-(4-nitrophenyl)ethyl)amino)methyl)-

InChi Key: GOKYHQGRIIXMNE-UHFFFAOYSA-N

InChi Code: InChI=1S/C19H25N3O4/c1-20(2)17-11-15(19(23)18(12-17)26-4)13-21(3)10-9-14-5-7-16(8-6-14)22(24)25/h5-8,11-12,23H,9-10,13H2,1-4H3

SMILES Code: OC1=C(CN(C)CCC2=CC=C([N+]([O-])=O)C=C2)C=C(N(C)C)C=C1OC

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >3 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.03.00

More Info: CDC25 dual-specificity phosphatases are essential regulators that dephosphorylate and activate cyclin-dependent kinase/cyclin complexes at key transitions of the cell cycle. CDC25 activity is currently considered to be an interesting target for the development of new antiproliferative agents

Product Data:
Product Data
Instruction:
View handling instruction
Biological target: BN82002 is a potent, selective and irreversible inhibitor of CDC25 phosphatase family.
In vitro activity: BN82002 dose-dependently down-regulated mRNA levels of nitric oxide synthase, tumor necrosis factor-α, and cyclooxygenase-2. The nuclear translocation of nuclear factor (NF)-κB (p65 and p50) was also blocked by BN82002 in RAW265.7 cells stimulated by LPS. Reference: Biochem Pharmacol. 2019 Jun;164:216-227. https://pubmed.ncbi.nlm.nih.gov/30980807/
In vivo activity: On the basis of the in vitro results, the activity of BN82002 was evaluated in athymic mice xenografted with the human pancreatic cell MIA PaCa-2. BN82002 stabilized tumor growth during the once daily × 10 treatment and induced a delay in the once weekly × 3 schedule. Reference: Cancer Res. 2004 May 1;64(9):3320-5. https://pubmed.ncbi.nlm.nih.gov/15126376/

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 150.0 417.33

Preparing Stock Solutions

The following data is based on the product molecular weight 359.43 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol: 1. Kim HG, Yang WS, Hong YH, Kweon DH, Lee J, Kim S, Cho JY. Anti-inflammatory functions of the CDC25 phosphatase inhibitor BN82002 via targeting AKT2. Biochem Pharmacol. 2019 Jun;164:216-227. doi: 10.1016/j.bcp.2019.04.007. Epub 2019 Apr 10. PMID: 30980807. 2. Brezak MC, Quaranta M, Mondésert O, Galcera MO, Lavergne O, Alby F, Cazales M, Baldin V, Thurieau C, Harnett J, Lanco C, Kasprzyk PG, Prevost GP, Ducommun B. A novel synthetic inhibitor of CDC25 phosphatases: BN82002. Cancer Res. 2004 May 1;64(9):3320-5. doi: 10.1158/0008-5472.can-03-3984. PMID: 15126376. 3. Brezak MC, Quaranta M, Mondésert O, Galcera MO, Lavergne O, Alby F, Cazales M, Baldin V, Thurieau C, Harnett J, Lanco C, Kasprzyk PG, Prevost GP, Ducommun B. A novel synthetic inhibitor of CDC25 phosphatases: BN82002. Cancer Res. 2004 May 1;64(9):3320-5. doi: 10.1158/0008-5472.can-03-3984. PMID: 15126376.
In vitro protocol: 1. Kim HG, Yang WS, Hong YH, Kweon DH, Lee J, Kim S, Cho JY. Anti-inflammatory functions of the CDC25 phosphatase inhibitor BN82002 via targeting AKT2. Biochem Pharmacol. 2019 Jun;164:216-227. doi: 10.1016/j.bcp.2019.04.007. Epub 2019 Apr 10. PMID: 30980807. 2. Brezak MC, Quaranta M, Mondésert O, Galcera MO, Lavergne O, Alby F, Cazales M, Baldin V, Thurieau C, Harnett J, Lanco C, Kasprzyk PG, Prevost GP, Ducommun B. A novel synthetic inhibitor of CDC25 phosphatases: BN82002. Cancer Res. 2004 May 1;64(9):3320-5. doi: 10.1158/0008-5472.can-03-3984. PMID: 15126376.
In vivo protocol: 1. Brezak MC, Quaranta M, Mondésert O, Galcera MO, Lavergne O, Alby F, Cazales M, Baldin V, Thurieau C, Harnett J, Lanco C, Kasprzyk PG, Prevost GP, Ducommun B. A novel synthetic inhibitor of CDC25 phosphatases: BN82002. Cancer Res. 2004 May 1;64(9):3320-5. doi: 10.1158/0008-5472.can-03-3984. PMID: 15126376.

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1: Brenner AK, Reikvam H, Rye KP, Hagen KM, Lavecchia A, Bruserud Ø. CDC25 Inhibition in Acute Myeloid Leukemia-A Study of Patient Heterogeneity and the Effects of Different Inhibitors. Molecules. 2017 Mar 11;22(3). pii: E446. doi: 10.3390/molecules22030446. PubMed PMID: 28287460; PubMed Central PMCID: PMC6155411.

2: Yamashita Y, Kasugai I, Sato M, Tanuma N, Sato I, Nomura M, Yamashita K, Sonoda Y, Kumabe T, Tominaga T, Katakura R, Shima H. CDC25A mRNA levels significantly correlate with Ki-67 expression in human glioma samples. J Neurooncol. 2010 Oct;100(1):43-9. doi: 10.1007/s11060-010-0147-3. Epub 2010 Mar 10. PubMed PMID: 20217459.

3: Contour-Galcéra MO, Lavergne O, Brezak MC, Ducommun B, Prévost G. Synthesis of small molecule CDC25 phosphatases inhibitors. Bioorg Med Chem Lett. 2004 Dec 6;14(23):5809-12. PubMed PMID: 15501045.

4: Brezak MC, Quaranta M, Mondésert O, Galcera MO, Lavergne O, Alby F, Cazales M, Baldin V, Thurieau C, Harnett J, Lanco C, Kasprzyk PG, Prevost GP, Ducommun B. A novel synthetic inhibitor of CDC25 phosphatases: BN82002. Cancer Res. 2004 May 1;64(9):3320-5. PubMed PMID: 15126376.