JNJ0966 | CAS# 315705-75-0 | MMP-9 Inhibitor

JNJ0966
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WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 564581

CAS#: 315705-75-0

Description: JNJ0966 is a highly selective allosteric inhibitor of matrix metalloproteinase-9 (MMP-9).

Chemical Structure

JNJ0966

CAS# 315705-75-0

Product data Instruction

Theoretical Analysis

MedKoo Cat#: 564581
Name: JNJ0966
CAS#: 315705-75-0
Chemical Formula: C16H16N4O2S2
Exact Mass: 360.07
Molecular Weight: 360.450
Elemental Analysis: C, 53.32; H, 4.47; N, 15.54; O, 8.88; S, 17.79

Price and Availability

Size	Price	Availability
25mg	USD 250	2 Weeks
50mg	USD 450	2 Weeks
100mg	USD 750	2 Weeks
200mg	USD 1250	2 Weeks
500mg	USD 2650	2 Weeks
1g	USD 3750	2 Weeks
2g	USD 6250	2 Weeks
Bulk inquiry Welcome, Customer: Please do not inquire quote if your intended use is for a patient since our products are for research use and for chemical synthesis use, not for human use . For in-stock products, we listed price in the web page. You may inquire prices for which sizes were not listed. If no price is listed, this means the product is not in stock at the moment, which may be available via custom synthesis. For cost-effective reason, minimum order of 1g is requested (typically very expensive). The lead time is usually 2-4 months. Quote of less than 1g will not be provided. MedKoo may not offer quote for below reasons: (1) current available synthetic method appears to be extremely expensive which is obviously not affordable to most customers; (2) Key raw material to make the product is temporally not available; (3) DEA controlled substances; (4) the product is under patent protection in customer’s country.

Synonym: JNJ-0966; JNJ 0966; JNJ0966

IUPAC/Chemical Name: N-(2-((2-Methoxyphenyl)amino)-4'-methyl-[4,5'-bithiazol]-2'-yl)acetamide

InChi Key: ZADCDCMLLGDCRM-UHFFFAOYSA-N

InChi Code: InChI=1S/C16H16N4O2S2/c1-9-14(24-16(17-9)18-10(2)21)12-8-23-15(20-12)19-11-6-4-5-7-13(11)22-3/h4-8H,1-3H3,(H,19,20)(H,17,18,21)

SMILES Code: CC(NC1=NC(C)=C(C2=CSC(NC3=CC=CC=C3OC)=N2)S1)=O

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >3 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Product Data:

Product Data

Instruction:

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Biological target:	JNJ0966 is a highly selective MMP-9 zymogen inhibitor with an IC50 of 440 nM.
In vitro activity:	The efficacy of the inhibitor behaves in a dose-dependent manner, and this study determined that a 2-h pre-treatment with 20 µM of JNJ0966 could prevent the elongation of rat LECs that have been exposed to 6 ng/mL of TGF-β for 48 h. Immunofluorescence analysis was conducted to further confirm the efficacy of JNJ0966. Reference: Int J Mol Sci. 2021 Nov 5;22(21):11988. https://pubmed.ncbi.nlm.nih.gov/34769418/
In vivo activity:	JNJ0966 was efficacious in reducing disease severity in a mouse experimental autoimmune encephalomyelitis model, demonstrating the viability of this therapeutic approach. Reference: J Biol Chem. 2017 Oct 27;292(43):17963-17974. https://pubmed.ncbi.nlm.nih.gov/28860188/

Solubility Data

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	63.3	175.72

Preparing Stock Solutions

The following data is based on the product molecular weight 360.45 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:	1. Liu ZZG, Taiyab A, West-Mays JA. MMP9 Differentially Regulates Proteins Involved in Actin Polymerization and Cell Migration during TGF-β-Induced EMT in the Lens. Int J Mol Sci. 2021 Nov 5;22(21):11988. doi: 10.3390/ijms222111988. PMID: 34769418; PMCID: PMC8584335. 2. Boon L, Ugarte-Berzal E, Martens E, Fiten P, Vandooren J, Janssens R, Blanter M, Yu K, Boon M, Struyf S, Proost P, Opdenakker G. Citrullination as a novel posttranslational modification of matrix metalloproteinases. Matrix Biol. 2021 Jan;95:68-83. doi: 10.1016/j.matbio.2020.10.005. Epub 2020 Nov 4. PMID: 33157227. 3. Scannevin RH, Alexander R, Haarlander TM, Burke SL, Singer M, Huo C, Zhang YM, Maguire D, Spurlino J, Deckman I, Carroll KI, Lewandowski F, Devine E, Dzordzorme K, Tounge B, Milligan C, Bayoumy S, Williams R, Schalk-Hihi C, Leonard K, Jackson P, Todd M, Kuo LC, Rhodes KJ. Discovery of a highly selective chemical inhibitor of matrix metalloproteinase-9 (MMP-9) that allosterically inhibits zymogen activation. J Biol Chem. 2017 Oct 27;292(43):17963-17974. doi: 10.1074/jbc.M117.806075. Epub 2017 Aug 31. PMID: 28860188; PMCID: PMC5663893.
In vitro protocol:	1. Liu ZZG, Taiyab A, West-Mays JA. MMP9 Differentially Regulates Proteins Involved in Actin Polymerization and Cell Migration during TGF-β-Induced EMT in the Lens. Int J Mol Sci. 2021 Nov 5;22(21):11988. doi: 10.3390/ijms222111988. PMID: 34769418; PMCID: PMC8584335. 2. Boon L, Ugarte-Berzal E, Martens E, Fiten P, Vandooren J, Janssens R, Blanter M, Yu K, Boon M, Struyf S, Proost P, Opdenakker G. Citrullination as a novel posttranslational modification of matrix metalloproteinases. Matrix Biol. 2021 Jan;95:68-83. doi: 10.1016/j.matbio.2020.10.005. Epub 2020 Nov 4. PMID: 33157227.
In vivo protocol:	1. Scannevin RH, Alexander R, Haarlander TM, Burke SL, Singer M, Huo C, Zhang YM, Maguire D, Spurlino J, Deckman I, Carroll KI, Lewandowski F, Devine E, Dzordzorme K, Tounge B, Milligan C, Bayoumy S, Williams R, Schalk-Hihi C, Leonard K, Jackson P, Todd M, Kuo LC, Rhodes KJ. Discovery of a highly selective chemical inhibitor of matrix metalloproteinase-9 (MMP-9) that allosterically inhibits zymogen activation. J Biol Chem. 2017 Oct 27;292(43):17963-17974. doi: 10.1074/jbc.M117.806075. Epub 2017 Aug 31. PMID: 28860188; PMCID: PMC5663893.

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1: Scannevin RH, Alexander R, Haarlander TM, Burke SL, Singer M, Huo C, Zhang YM, Maguire D, Spurlino J, Deckman I, Carroll KI, Lewandowski F, Devine E, Dzordzorme K, Tounge B, Milligan C, Bayoumy S, Williams R, Schalk-Hihi C, Leonard K, Jackson P, Todd M, Kuo LC, Rhodes KJ. Discovery of a highly selective chemical inhibitor of matrix metalloproteinase-9 (MMP-9) that allosterically inhibits zymogen activation. J Biol Chem. 2017 Oct 27;292(43):17963-17974. doi: 10.1074/jbc.M117.806075. Epub 2017 Aug 31. PubMed PMID: 28860188; PubMed Central PMCID: PMC5663893.

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