Wilforlide A
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MedKoo CAT#: 555369

CAS#: 84104-71-2

Description: Wilforlide A, also known as Regelide and Abruslactone A, is an active compound in Tripterygium wilfordii Hook F (TW) which is a traditional Chinese medicine for treatment of autoimmune diseases, is a quality control marker for TW product.


Chemical Structure

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Wilforlide A
CAS# 84104-71-2

Theoretical Analysis

MedKoo Cat#: 555369
Name: Wilforlide A
CAS#: 84104-71-2
Chemical Formula: C30H46O3
Exact Mass: 454.34
Molecular Weight: 454.695
Elemental Analysis: C, 79.25; H, 10.20; O, 10.56

Price and Availability

Size Price Availability Quantity
100mg USD 850
200mg USD 1250
500mg USD 1950
1g USD 2950
2g USD 4950
5g USD 9650
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Synonym: Wilforlide A; Regelide; Abruslactone A;

IUPAC/Chemical Name: (2S,5S,5aR,7aS,7bR,9aR,11S,13aR,13bR,15bS)-11-hydroxy-2,5a,7a,7b,10,10,13a-heptamethyl-1,5,5a,6,7,7a,7b,8,9,9a,10,11,12,13,13a,13b,14,15b-octadecahydro-2,5-methanochryseno[2,1-c]oxepin-3(2H)-one

InChi Key: HHQJBWYXBWOFJY-YLXTXNMFSA-N

InChi Code: InChI=1S/C30H46O3/c1-25(2)20-10-13-30(7)21(28(20,5)12-11-22(25)31)9-8-18-19-16-26(3)17-23(33-24(26)32)27(19,4)14-15-29(18,30)6/h8,19-23,31H,9-17H2,1-7H3/t19-,20-,21+,22-,23-,26-,27+,28-,29+,30+/m0/s1

SMILES Code: O=C1[C@@](C2)(C)C[C@]3([H])[C@](CC[C@@]4(C)[C@]5(C)CC[C@@]6([H])C(C)(C)[C@@H](O)CC[C@]6(C)[C@@]5([H])CC=C43)(C)[C@@]2([H])O1

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >3 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.03.00

More Info:

Biological target:
In vitro activity:
In vivo activity:

Preparing Stock Solutions

The following data is based on the product molecular weight 454.70 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
In vitro protocol:
In vivo protocol:

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1: Wang Z, Yeung S, Chen S, Moatazedi Y, Chow MSS. Bioavailability of wilforlide A in mice and its concentration determination using an HPLC-APCI-MS/MS method. J Chromatogr B Analyt Technol Biomed Life Sci. 2018 Jul 15;1090:65-72. doi: 10.1016/j.jchromb.2018.05.018. Epub 2018 May 16. PubMed PMID: 29787994.

2: Zhang Q, Li Y, Liu M, Duan J, Zhou X, Zhu H. Compatibility with Panax notoginseng and Rehmannia glutinosa Alleviates the Hepatotoxicity and Nephrotoxicity of Tripterygium wilfordii via Modulating the Pharmacokinetics of Triptolide. Int J Mol Sci. 2018 Jan 19;19(1). pii: E305. doi: 10.3390/ijms19010305. PubMed PMID: 29351251; PubMed Central PMCID: PMC5796250.

3: Fan D, Parhira S, Zhu GY, Jiang ZH, Bai LP. Triterpenoids from the stems of Tripterygium regelii. Fitoterapia. 2016 Sep;113:69-73. doi: 10.1016/j.fitote.2016.07.006. Epub 2016 Jul 15. PubMed PMID: 27425447.

4: Zhai JX, Liu W. [Determination of Triptolide and Wilforlide A in Biological Samples by LC-MS/MS]. Fa Yi Xue Za Zhi. 2015 Dec;31(6):445-9, 453. Chinese. PubMed PMID: 27141802.

5: Su P, Cheng Q, Wang X, Cheng X, Zhang M, Tong Y, Li F, Gao W, Huang L. Characterization of eight terpenoids from tissue cultures of the Chinese herbal plant, Tripterygium wilfordii, by high-performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry. Biomed Chromatogr. 2014 Sep;28(9):1183-92. doi: 10.1002/bmc.3140. PubMed PMID: 25237706.

6: Zhou D, Zhang L, Sun W. [Study of wilforlide on foot cells and renal tubular epithelial cells protection]. Zhongguo Zhong Xi Yi Jie He Za Zhi. 2011 Apr;31(4):569-71. Review. Chinese. PubMed PMID: 21608234.

7: Xue M, Jiang ZZ, Liu JP, Zhang LY, Wang T, Wang H, Liu L, Zhou ZX. Comparative study on the anti-inflammatory and immune suppressive effect of Wilforlide A. Fitoterapia. 2010 Dec;81(8):1109-12. doi: 10.1016/j.fitote.2010.07.007. Epub 2010 Jul 16. PubMed PMID: 20621167.

8: Luo XL, Shao Q, Qu HB, Cheng YY. Simple method for determination of five terpenoids from different parts of Tripterygium wilfordii and its preparations by HPLC coupled with evaporative light scattering detection. J Sep Sci. 2007 Jun;30(9):1284-91. PubMed PMID: 17623469.

9: Yang JH, Luo SD, Wang YS, Zhao JF, Zhang HB, Li L. Triterpenes from Tripterygium wilfordii Hook. J Asian Nat Prod Res. 2006 Jul-Aug;8(5):425-9. PubMed PMID: 16864458.

10: Yao J, Zhang L, Zhao X, Hu L, Jiang Z. Simultaneous determination of triptolide, wilforlide A and triptonide in human plasma by high-performance liquid chromatography-electrospray ionization mass spectrometry. Biol Pharm Bull. 2006 Jul;29(7):1483-6. PubMed PMID: 16819194.

11: Lu Y, Zheng Q, Lu D, Ma P, Chen Y. [Fine stereo structure for natural organic molecules, a preliminary study. II. Melting point influenced by structure factors]. Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 1995 Jun;17(3):192-6. Chinese. PubMed PMID: 8706154.

12: Yan XL, Liao SX, Liang HQ, Zhou P. [Studies on the chemical constituents of Euonymus mupinensis]. Yao Xue Xue Bao. 1993;28(9):684-9. Chinese. PubMed PMID: 8010015.

13: Zhang CP. [Studies on triterpenoids of total glucosides of Tripterygium wilfordii (T II)]. Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 1989 Oct;11(5):322-5. Chinese. PubMed PMID: 2534610.

14: Gao QP. [Chemical constituents of heiman (Tripterygium regelii)]. Zhong Yao Tong Bao. 1987 Jan;12(1):41-2. Chinese. PubMed PMID: 2954704.