WARNING: This product is for research use only, not for human or veterinary use.
MedKoo CAT#: 563706
CAS#: 92557-80-7
Description: 5-FAMSE is a cell-permeable green fluorescent labeling probe, demostrating excitation and emission maxima are 494 and 520 nm, respectively.
MedKoo Cat#: 563706
Name: 5-FAMSE
CAS#: 92557-80-7
Chemical Formula: C25H15NO9
Exact Mass: 473.0747
Molecular Weight: 473.39
Elemental Analysis: C, 63.43; H, 3.19; N, 2.96; O, 30.42
Synonym: 5-FAMSE; 5 FAMSE; 5FAMSE; CFSE; 5-FAM-SE;
IUPAC/Chemical Name: 2,5-dioxopyrrolidin-1-yl 3',6'-dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-5-carboxylate
InChi Key: GECIDMICWWDIBO-UHFFFAOYSA-N
InChi Code: InChI=1S/C25H15NO9/c27-13-2-5-17-19(10-13)33-20-11-14(28)3-6-18(20)25(17)16-4-1-12(9-15(16)24(32)34-25)23(31)35-26-21(29)7-8-22(26)30/h1-6,9-11,27-28H,7-8H2
SMILES Code: OC1=CC=C2C3(C4=C(OC2=C1)C=C(O)C=C4)C5=C(C(O3)=O)C=C(C(ON6C(CCC6=O)=O)=O)C=C5
Appearance: Solid powder
Purity: >98% (or refer to the Certificate of Analysis)
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility: Soluble in DMSO
Shelf Life: >3 years if stored properly
Drug Formulation: This drug may be formulated in DMSO
Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code: 2934.99.03.00
The following data is based on the product molecular weight 473.39 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
1: Zahng J, Liu W, Liu L. [Study on 5-carboxyfluorescein N-succinimidyl ester in detecting the degree of cross-linked allogenic tissue]. Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2010 Aug;27(4):816-9. Chinese. PubMed PMID: 20842851.
