WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 563560

CAS#: 79955-27-4

Description: 5-CFDA is a cell membranes permeatable non-fluorescent prodrug of 5-carboxyfluorescein. It shows strongly fluorescent to differentiate viable cells from apoptotic cells after being enzymatically hydrolyzed inside cells.

Chemical Structure

CAS# 79955-27-4

Theoretical Analysis

MedKoo Cat#: 563560
Name: 5-CFDA
CAS#: 79955-27-4
Chemical Formula: C25H16O9
Exact Mass: 460.0794
Molecular Weight: 460.39
Elemental Analysis: C, 65.22; H, 3.50; O, 31.28

Price and Availability

Size Price Availability Quantity
25.0mg USD 260.0 2 Weeks
100.0mg USD 510.0 2 Weeks
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Synonym: 5-CFDA; 5 CFDA; 5CFDA; CFDA-5; CFDA 5; CFDA5;

IUPAC/Chemical Name: 3',6'-Diacetyloxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-5-carboxylic acid


InChi Code: InChI=1S/C25H16O9/c1-12(26)31-15-4-7-19-21(10-15)33-22-11-16(32-13(2)27)5-8-20(22)25(19)18-6-3-14(23(28)29)9-17(18)24(30)34-25/h3-11H,1-2H3,(H,28,29)

SMILES Code: O=C(C1=CC=C(C2=C1)C3(C4=C(OC5=C3C=CC(OC(C)=O)=C5)C=C(OC(C)=O)C=C4)OC2=O)O

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >3 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.03.00

Preparing Stock Solutions

The following data is based on the product molecular weight 460.39 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL

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1: Kasaian J, Mosaffa F, Behravan J, Masullo M, Piacente S, Iranshahi M. Modulation of Multidrug Resistance Protein 2 Efflux in the Cisplatin Resistance Human Ovarian Carcinoma Cells A2780/RCIS by Sesquiterpene Coumarins. Phytother Res. 2016 Jan;30(1):84-9. doi: 10.1002/ptr.5504. Epub 2015 Oct 27. PubMed PMID: 26503061.

2: Plominsky ÁM, Delherbe N, Mandakovic D, Riquelme B, González K, Bergman B, Mariscal V, Vásquez M. Intercellular transfer along the trichomes of the invasive terminal heterocyst forming cyanobacterium Cylindrospermopsis raciborskii CS-505. FEMS Microbiol Lett. 2015 Mar;362(5). pii: fnu009. doi: 10.1093/femsle/fnu009. Epub 2014 Dec 4. PubMed PMID: 25757729.

3: Wang CY, Wang DL, Tao XH, Yang LL, Chen JP, Cao Y. [Influence of huatanjiangqi prescription on multidrug resistance-associated protein 1 in human bronchial epithelial cells]. Zhong Yao Cai. 2014 Feb;37(2):280-3. Chinese. PubMed PMID: 25095351.

4: Gorokhova E, Mattsson L, Sundström AM. A comparison of TO-PRO-1 iodide and 5-CFDA-AM staining methods for assessing viability of planktonic algae with epifluorescence microscopy. J Microbiol Methods. 2012 Jun;89(3):216-21. doi: 10.1016/j.mimet.2012.03.005. Epub 2012 Mar 14. PubMed PMID: 22446098.

5: Jekerle V, Klinkhammer W, Scollard DA, Breitbach K, Reilly RM, Piquette-Miller M, Wiese M. In vitro and in vivo evaluation of WK-X-34, a novel inhibitor of P-glycoprotein and BCRP, using radio imaging techniques. Int J Cancer. 2006 Jul 15;119(2):414-22. PubMed PMID: 16646006.

6: Jekerle V, Klinkhammer W, Reilly RM, Piquette-Miller M, Wiese M. Novel tetrahydroisoquinolin-ethyl-phenylamine based multidrug resistance inhibitors with broad-spectrum modulating properties. Cancer Chemother Pharmacol. 2007 Jan;59(1):61-9. Epub 2006 Apr 25. PubMed PMID: 16636798.

7: Sugawara S, Hosono M, Ogawa Y, Takayanagi M, Nitta K. Catfish egg lectin causes rapid activation of multidrug resistance 1 P-glycoprotein as a lipid translocase. Biol Pharm Bull. 2005 Mar;28(3):434-41. PubMed PMID: 15744065.

8: Fuller ME, Streger SH, Rothmel RK, Mailloux BJ, Hall JA, Onstott TC, Fredrickson JK, Balkwill DL, DeFlaun MF. Development of a vital fluorescent staining method for monitoring bacterial transport in subsurface environments. Appl Environ Microbiol. 2000 Oct;66(10):4486-96. PubMed PMID: 11010903; PubMed Central PMCID: PMC92329.


25.0mg / USD 260.0