LD 490
featured

    WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 598518

CAS#: 58336-35-9

Description: LD 490 is a laser dye.


Chemical Structure

img
LD 490
CAS# 58336-35-9

Theoretical Analysis

MedKoo Cat#: 598518
Name: LD 490
CAS#: 58336-35-9
Chemical Formula: C15H15NO2
Exact Mass: 241.11
Molecular Weight: 241.290
Elemental Analysis: C, 74.67; H, 6.27; N, 5.81; O, 13.26

Price and Availability

Size Price Availability Quantity
200mg USD 350 2 Weeks
1g USD 750 2 Weeks
Bulk inquiry

Synonym: LD 490; LD-490; LD490; C 6H; C-6H; C6H; Exciton LD 490;

IUPAC/Chemical Name: 2,3,6,7-tetrahydro-1H,5H,11H-pyrano[2,3-f]pyrido[3,2,1-ij]quinolin-11-one

InChi Key: MZSOXGPKUOAXNY-UHFFFAOYSA-N

InChi Code: InChI=1S/C15H15NO2/c17-13-6-5-11-9-10-3-1-7-16-8-2-4-12(14(10)16)15(11)18-13/h5-6,9H,1-4,7-8H2

SMILES Code: O=C1C=CC2=CC3=C4N(CCC3)CCCC4=C2O1

Appearance: Solid powder

Purity: >95% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >3 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.03.00

More Info:

Product Data:
Biological target: Coumarin 6H, a Coumarin (Coumarin (HY-N0709)) derivative, is a laser dye.
In vitro activity: A triaxial flashlamp-pumped dye laser has been used to perform laser damage testing of metal surfaces in the blue-green spectral region. Using LD490 laser dye, the laser produces 0.18-J, 0.5-microsec pulses at 492 nm. Reference: Appl Opt. 1982 Nov 15;21(22):4063-6. https://pubmed.ncbi.nlm.nih.gov/20401009/
In vivo activity: Herein, based on the combined effect of two different fluorescence quenching groups, this study rationally developed a novel fluorescent probe for hypochlorite with extremely low background fluorescence. Moreover, the fluorescence imaging different concentration of hypochlorite in zebrafish has been successfully conducted. Reference: Anal Biochem. 2020 Aug 1;602:113795. https://pubmed.ncbi.nlm.nih.gov/32473120/

Preparing Stock Solutions

The following data is based on the product molecular weight 241.29 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol: 1. Usta HM, Forough M, Persil Çetinkol Ö. Coumarin 6H-fused fluorescent probe for highly sensitive detection of coralyne using oligonucleotide-modified silver nanoparticles. Anal Bioanal Chem. 2022 Oct;414(24):7299-7313. doi: 10.1007/s00216-022-04282-2. Epub 2022 Aug 17. PMID: 35976422. 2. Marrs CD, Faith WN, Dancy JH, Porteus JO. Pulsed laser-induced damage of metals at 492 nm. Appl Opt. 1982 Nov 15;21(22):4063-6. doi: 10.1364/AO.21.004063. PMID: 20401009. 3. Han Z, Dong L, Sun F, Long L, Jiang S, Dai X, Zhang M. A novel fluorescent probe with extremely low background fluorescence for sensing hypochlorite in zebrafish. Anal Biochem. 2020 Aug 1;602:113795. doi: 10.1016/j.ab.2020.113795. Epub 2020 May 28. PMID: 32473120.
In vitro protocol: 1. Usta HM, Forough M, Persil Çetinkol Ö. Coumarin 6H-fused fluorescent probe for highly sensitive detection of coralyne using oligonucleotide-modified silver nanoparticles. Anal Bioanal Chem. 2022 Oct;414(24):7299-7313. doi: 10.1007/s00216-022-04282-2. Epub 2022 Aug 17. PMID: 35976422. 2. Marrs CD, Faith WN, Dancy JH, Porteus JO. Pulsed laser-induced damage of metals at 492 nm. Appl Opt. 1982 Nov 15;21(22):4063-6. doi: 10.1364/AO.21.004063. PMID: 20401009.
In vivo protocol: 1. Han Z, Dong L, Sun F, Long L, Jiang S, Dai X, Zhang M. A novel fluorescent probe with extremely low background fluorescence for sensing hypochlorite in zebrafish. Anal Biochem. 2020 Aug 1;602:113795. doi: 10.1016/j.ab.2020.113795. Epub 2020 May 28. PMID: 32473120.

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

1: Li H, Yin H, Liu X, Shi Y, Jin M, Ding D. An experimental and theoretical study of solvent hydrogen-bond-donating capacity effects on ultrafast intramolecular charge transfer of LD 490. Spectrochim Acta A Mol Biomol Spectrosc. 2017 Sep 5;184:270-276. doi: 10.1016/j.saa.2017.05.027. Epub 2017 May 15. PubMed PMID: 28525861.

2: Degirmencioglu A, Buturak A, Zencirci E, Karakus G, Güvenc TS, Akyol A, Esen A, Demirci Y, Sipahi I, Dagdelen S, Norgaz T, Gorgulu S. Comparison of Effects of Low- versus High-Dose Heparin on Access-Site Complications during Transradial Coronary Angiography: A Double-Blind Randomized Study. Cardiology. 2015;131(3):142-8. doi: 10.1159/000377621. Epub 2015 Apr 28. PubMed PMID: 25926088.

3: Qin X, Zerr DM, Kronman MP, Adler AL, Berry JE, Rich S, Buccat AM, Xu M, Englund JA. Comparison of molecular detection methods for pertussis in children during a state-wide outbreak. Ann Clin Microbiol Antimicrob. 2016 Apr 27;15:28. doi: 10.1186/s12941-016-0142-4. PubMed PMID: 27121506; PubMed Central PMCID: PMC4847268.

4: Wahman DG, Schrantz KA, Pressman JG. Determination of the effects of medium composition on the monochloramine disinfection kinetics of Nitrosomonas europaea by the propidium monoazide quantitative PCR and Live/Dead BacLight methods. Appl Environ Microbiol. 2010 Dec;76(24):8277-80. doi: 10.1128/AEM.01631-10. Epub 2010 Oct 15. PubMed PMID: 20952645; PubMed Central PMCID: PMC3008258.

5: Higashikawa FS, Cayuela ML, Roig A, Silva CA, Sánchez-Monedero MA. Matrix effect on the performance of headspace solid phase microextraction method for the analysis of target volatile organic compounds (VOCs) in environmental samples. Chemosphere. 2013 Nov;93(10):2311-8. doi: 10.1016/j.chemosphere.2013.08.023. Epub 2013 Sep 10. PubMed PMID: 24034827.

6: Wahman DG, Wulfeck-Kleier KA, Pressman JG. Monochloramine disinfection kinetics of Nitrosomonas europaea by propidium monoazide quantitative PCR and Live/dead BacLight methods. Appl Environ Microbiol. 2009 Sep;75(17):5555-62. doi: 10.1128/AEM.00407-09. Epub 2009 Jun 26. PubMed PMID: 19561179; PubMed Central PMCID: PMC2737938.

7: Zakomyrdina LN, Sakharova IS, Torchinskiĭ IuM. [Study of coenzyme reorientation in the active center of tryptophanase by linear dichroism method]. Mol Biol (Mosk). 1989 Nov-Dec;23(6):1596-602. Russian. PubMed PMID: 2698994.

8: Zakomirdina LN, Sakharova IS, Torchinsky YM. Linear dichroism studies of tryptophanase and its quasisubstrate complexes oriented in polyacrylamide gel. Eur J Biochem. 1990 Oct 5;193(1):243-7. Erratum in: Eur J Biochem 1991 Jan 1;195(1):287. PubMed PMID: 2121476.

9: Patel YM, Stram DO, Wilkens LR, Park SS, Henderson BE, Le Marchand L, Haiman CA, Murphy SE. The contribution of common genetic variation to nicotine and cotinine glucuronidation in multiple ethnic/racial populations. Cancer Epidemiol Biomarkers Prev. 2015 Jan;24(1):119-27. doi: 10.1158/1055-9965.EPI-14-0815. Epub 2014 Oct 7. PubMed PMID: 25293881; PubMed Central PMCID: PMC4294952.

10: Sudo K, Maekawa M, Watanabe H, Matsumoto K, Mori Y, Sasaki T, Kanno T. A case of immunoglobulin G conjugated with lactate dehydrogenase, producing both loss of enzyme activity and an abnormal isoenzyme pattern. Clin Chem. 1986 Jul;32(7):1420-2. PubMed PMID: 2941184.

11: Gowda BG, Melwanki MB, Seetharamappa J. Spectrophotometric determination of certain vicinal dihydroxybenzene derivatives in pharmaceutical preparations. Anal Sci. 2001 Apr;17(4):533-4. PubMed PMID: 11990572.

12: Chapman SJ, Khor CC, Vannberg FO, Rautanen A, Segal S, Moore CE, Davies RJ, Day NP, Peshu N, Crook DW, Berkley JA, Williams TN, Scott JA, Hill AV. NFKBIZ polymorphisms and susceptibility to pneumococcal disease in European and African populations. Genes Immun. 2010 Jun;11(4):319-25. doi: 10.1038/gene.2009.76. Epub 2009 Oct 1. PubMed PMID: 19798075; PubMed Central PMCID: PMC3051152.

13: Hayes DK. Action spectra for prevention or termination of diapause in three species of Lepidoptera. Prog Clin Biol Res. 1987;227A:239-47. PubMed PMID: 3601961.

14: Zheng L, Yin J, Wang L, Wang X, Shi Y, Shao A, Tang W, Ding G, Liu C, Chen S, Gu H. Interleukin 1B rs16944 G>A polymorphism was associated with a decreased risk of esophageal cancer in a Chinese population. Clin Biochem. 2013 Oct;46(15):1469-73. doi: 10.1016/j.clinbiochem.2013.05.050. Epub 2013 May 29. PubMed PMID: 23726808.

15: Streltsov SA, Gromyko AV, Oleinikov VA, Zhuze AL. The Hoechst 33258 covalent dimer covers a total turn of the double-stranded DNA. J Biomol Struct Dyn. 2006 Dec;24(3):285-302. Erratum in: J Biomol Struct Dyn. 2007 Feb;24(4):X. PubMed PMID: 17054387.

16: Chen JF, Wang HR, Yang S, Zhao YP, Zhao XH, Chen YC, DU QL, Liu SJ, Shen C, Xu YC. [An association study between transforming growth factor-β1 receptor 2 gene polymorphisms and essential hypertension]. Zhonghua Yu Fang Yi Xue Za Zhi. 2012 Sep;46(9):825-30. Chinese. PubMed PMID: 23157888.

17: Lambert W, Brisebois FJ, Wharton TJ, Carrier RC, Boyle D, Rowe BH. The effectiveness of low dose tranexamic acid in primary cardiac surgery. Can J Anaesth. 1998 Jun;45(6):571-4. PubMed PMID: 9669014.

18: Tang T, Joyner WL. Differential role of endothelial function on vasodilator responses in series-arranged arterioles. Microvasc Res. 1992 Jul;44(1):61-72. PubMed PMID: 1379321.

19: Ramakrishnan A, Gupta SK. Anaerobic biogranulation in a hybrid reactor treating phenolic waste. J Hazard Mater. 2006 Oct 11;137(3):1488-95. Epub 2006 May 2. PubMed PMID: 16762495.

20: Bang S, Nagata S, Onda M, Kreitman RJ, Pastan I. HA22 (R490A) is a recombinant immunotoxin with increased antitumor activity without an increase in animal toxicity. Clin Cancer Res. 2005 Feb 15;11(4):1545-50. PubMed PMID: 15746059.