DAMPA
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    WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 563418

CAS#: 19741-14-1

Description: DAMPA is a metabolite of methotrexate (MTX).

Chemical Structure

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DAMPA
CAS# 19741-14-1
Instruction

Theoretical Analysis

MedKoo Cat#: 563418
Name: DAMPA
CAS#: 19741-14-1
Chemical Formula: C15H15N7O2
Exact Mass: 325.13
Molecular Weight: 325.330
Elemental Analysis: C, 55.38; H, 4.65; N, 30.14; O, 9.84

Price and Availability

Size Price Availability Quantity
50mg USD 340 2 Weeks
500mg USD 1710 2 Weeks
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Synonym: DAMPA; Deoxyaminopteroic acid; NSC131463; NSC-131463; NSC 131463;

IUPAC/Chemical Name: 4-[(2,4-Diaminopteridin-6-yl)methyl-methylamino]benzoic acid

InChi Key: LWCXZSDKANNOAR-UHFFFAOYSA-N

InChi Code: InChI=1S/C15H15N7O2/c1-22(10-4-2-8(3-5-10)14(23)24)7-9-6-18-13-11(19-9)12(16)20-15(17)21-13/h2-6H,7H2,1H3,(H,23,24)(H4,16,17,18,20,21)

SMILES Code: O=C(O)C1=CC=C(N(CC2=NC3=C(N)N=C(N)N=C3N=C2)C)C=C1

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >3 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.03.00

More Info:

Biological target:
In vitro activity:
In vivo activity:

Preparing Stock Solutions

The following data is based on the product molecular weight 325.33 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
In vitro protocol:
In vivo protocol:

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5: Schofield RC, Ramanathan LV, Murata K, Fleisher M, Pessin MS, Carlow DC. Development of an Assay for Methotrexate and Its Metabolites 7-Hydroxy Methotrexate and DAMPA in Serum by LC-MS/MS. Methods Mol Biol. 2016;1383:213-22. doi: 10.1007/978-1-4939-3252-8_23. PubMed PMID: 26660190.

6: Schofield RC, Ramanathan LV, Murata K, Grace M, Fleisher M, Pessin MS, Carlow DC. Development and validation of a turbulent flow chromatography and tandem mass spectrometry method for the quantitation of methotrexate and its metabolites 7-hydroxy methotrexate and DAMPA in serum. J Chromatogr B Analyt Technol Biomed Life Sci. 2015 Oct 1;1002:169-75. doi: 10.1016/j.jchromb.2015.08.025. Epub 2015 Aug 22. PubMed PMID: 26322588; PubMed Central PMCID: PMC4982141.

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10: Zhao SS, Bichelberger MA, Colin DY, Robitaille R, Pelletier JN, Masson JF. Monitoring methotrexate in clinical samples from cancer patients during chemotherapy with a LSPR-based competitive sensor. Analyst. 2012 Oct 21;137(20):4742-50. doi: 10.1039/c2an35839e. PubMed PMID: 22943049.

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12: Uchiyama M, Matsumoto T, Matsumoto T, Jimi S, Takamatsu Y, Tamura K, Hara S. Simple and sensitive HPLC method for the fluorometric determination of methotrexate and its major metabolites in human plasma by post-column photochemical reaction. Biomed Chromatogr. 2012 Jan;26(1):76-80. doi: 10.1002/bmc.1628. Epub 2011 Mar 25. PubMed PMID: 21437923.

13: Cheng HL, Chiou SS, Liao YM, Lu CY, Chen YL, Wu SM. Analysis of methotrexate and its eight metabolites in cerebrospinal fluid by solid-phase extraction and triple-stacking capillary electrophoresis. Anal Bioanal Chem. 2010 Nov;398(5):2183-90. doi: 10.1007/s00216-010-4152-3. Epub 2010 Sep 6. PubMed PMID: 20820994.

14: Kumar VS, Law T, Kellogg M. Liquid chromatography-tandem mass spectrometry (LC-MS-MS) method for monitoring methotrexate in the setting of carboxypeptidase-G2 therapy. Methods Mol Biol. 2010;603:359-63. doi: 10.1007/978-1-60761-459-3_34. PubMed PMID: 20077087.

15: Nduati E, Hunt S, Kamau EM, Nzila A. 2,4-diaminopteridine-based compounds as precursors for de novo synthesis of antifolates: a novel class of antimalarials. Antimicrob Agents Chemother. 2005 Sep;49(9):3652-7. PubMed PMID: 16127035; PubMed Central PMCID: PMC1195384.

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