Pyronin Y
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    WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 592290

CAS#: 92-32-0

Description: Pyronine is a Xanthene dye used as a bacterial and biological stain.


Chemical Structure

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Pyronin Y
CAS# 92-32-0

Theoretical Analysis

MedKoo Cat#: 592290
Name: Pyronin Y
CAS#: 92-32-0
Chemical Formula: C17H19ClN2O
Exact Mass: 0.00
Molecular Weight: 302.800
Elemental Analysis: C, 67.43; H, 6.32; Cl, 11.71; N, 9.25; O, 5.28

Price and Availability

Size Price Availability Quantity
1g USD 230
5g USD 360
10g USD 560
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Synonym: Pyronine; Pyronin Y

IUPAC/Chemical Name: Ammonium, (6-(dimethylamino)-3H-xanten-3-ylidene)dimethyl-, chloride

InChi Key: INCIMLINXXICKS-UHFFFAOYSA-M

InChi Code: InChI=1S/C17H19N2O.ClH/c1-18(2)14-7-5-12-9-13-6-8-15(19(3)4)11-17(13)20-16(12)10-14;/h5-11H,1-4H3;1H/q+1;/p-1

SMILES Code: C/[N+](C)=C1C=CC2=CC3=C(C=C(N(C)C)C=C3)OC2=C/1.[Cl-]

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >3 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.03.00

More Info:

Product Data:
Biological target: Pyronin Y is a dye.
In vitro activity: In this study, pyronin Y was used as a staining agent for cellular RNA in Chinese hamster ovary (CHO) cells. Pyronin Y was used to assess the stainability of RNA in different cellular conditions, such as mitosis (M), G2 phase, and interphase cells exposed to hyperthermia. Pyronin Y was used to as a sensitive probe to investigate the effects of mitosis and hyperthermia on the stainability of RNA in CHO cells. Reference: Exp Cell Res. 1988 Dec;179(2):535-44. https://pubmed.ncbi.nlm.nih.gov/2461311/
In vivo activity: Pyronin assessed primitive hematopoietic stem cells (PHSC) during G(0) and G(1). It differentiated the Rh(lo)Lin(-)Kit(+)Sca-1(+) PHSC subset from more mature cells. Further, it revealed diverse functionality within the Rh(lo)Ho(lo/sp)Lin(-)Sca-1(+) PHSC group, with PY(lo)Rh(lo)Ho(lo/sp) PHSC having a higher competitive repopulating unit (CRU) frequency than PY(hi) PHSC at G(1). Pyronin showed dose-dependent cytotoxicity, impacting non-early hematopoietic cells while sparing the early HSC compartment. Reference: Exp Hematol. 2001 Sep;29(9):1109-16. https://pubmed.ncbi.nlm.nih.gov/11532352/

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 25.0 82.56
Water 4.0 13.21

Preparing Stock Solutions

The following data is based on the product molecular weight 302.80 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol: 1. Salci A, Toprak M. Spectroscopic investigations on the binding of Pyronin Y to human serum albumin. J Biomol Struct Dyn. 2017 Jan;35(1):8-16. doi: 10.1080/07391102.2015.1128357. Epub 2016 Feb 17. PMID: 26646531. 2. Traganos F, Crissman HA, Darzynkiewicz Z. Staining with pyronin Y detects changes in conformation of RNA during mitosis and hyperthermia of CHO cells. Exp Cell Res. 1988 Dec;179(2):535-44. doi: 10.1016/0014-4827(88)90291-1. PMID: 2461311. In vivo study 1. Hüttmann A, Liu SL, Boyd AW, Li CL. Functional heterogeneity within rhodamine123(lo) Hoechst33342(lo/sp) primitive hemopoietic stem cells revealed by pyronin Y. Exp Hematol. 2001 Sep;29(9):1109-16. doi: 10.1016/s0301-472x(01)00684-1. PMID: 11532352.
In vitro protocol: 1. Salci A, Toprak M. Spectroscopic investigations on the binding of Pyronin Y to human serum albumin. J Biomol Struct Dyn. 2017 Jan;35(1):8-16. doi: 10.1080/07391102.2015.1128357. Epub 2016 Feb 17. PMID: 26646531. 2. Traganos F, Crissman HA, Darzynkiewicz Z. Staining with pyronin Y detects changes in conformation of RNA during mitosis and hyperthermia of CHO cells. Exp Cell Res. 1988 Dec;179(2):535-44. doi: 10.1016/0014-4827(88)90291-1. PMID: 2461311.
In vivo protocol: 1. Hüttmann A, Liu SL, Boyd AW, Li CL. Functional heterogeneity within rhodamine123(lo) Hoechst33342(lo/sp) primitive hemopoietic stem cells revealed by pyronin Y. Exp Hematol. 2001 Sep;29(9):1109-16. doi: 10.1016/s0301-472x(01)00684-1. PMID: 11532352.

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