pCAME
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    WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 563226

CAS#: 19367-38-5

Description: pCAME is an inducer of ectopic tail formation of zebrafish.

Chemical Structure

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pCAME
CAS# 19367-38-5
Product data Instruction

Theoretical Analysis

MedKoo Cat#: 563226
Name: pCAME
CAS#: 19367-38-5
Chemical Formula: C10H10O3
Exact Mass: 178.06
Molecular Weight: 178.187
Elemental Analysis: C, 67.41; H, 5.66; O, 26.94

Price and Availability

This product is not in stock, which may be available by custom synthesis. For cost-effective reason, minimum order is 1g (price is usually high, lead time is 2~3 months, depending on the technical challenge). Quote less than 1g will not be provided. To request quote, please email to sales @medkoo.com or click below button.
Note: Price will be listed if it is available in the future.

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Synonym: pCAME; p-CAME; p CAME;

IUPAC/Chemical Name: 3-(4-Hydroxy-phenyl)-acrylic acid methyl ester

InChi Key: NITWSHWHQAQBAW-QPJJXVBHSA-N

InChi Code: InChI=1S/C10H10O3/c1-13-10(12)7-4-8-2-5-9(11)6-3-8/h2-7,11H,1H3/b7-4+

SMILES Code: O=C(OC)/C=C/C1=CC=C(O)C=C1

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >3 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.03.00

More Info:

Product Data:
Product Data
Instruction:
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Biological target: (E)-Methyl 4-coumarate (Methyl 4-hydroxycinnamate), found in several plants, such as Allium cepa or Morinda citrifolia L. leaves. (E)-Methyl 4-coumarate cooperates with Carnosic Acid in inducing apoptosis and killing acute myeloid leukemia cells, but not normal peripheral blood mononuclear cells.
In vitro activity: pCAME could inhibit the proliferation, would healing, migration and tube formation of HUVECs, disrupt the physiological formation of intersegmental vessels (ISVs) and the subintestinal vessels (SIVs) of zebrafish embryos, and inhibit tumor angiogenesis in the zebrafish cell-line derived xenograft (zCDX) model of SGC-7901 in a dose-dependent manner. Reference: Phytomedicine. 2018 Sep 15;48:10-20. https://pubmed.ncbi.nlm.nih.gov/30195867/
In vivo activity: Hence, the present study is aimed to determine the protective effect of PCAME against F- induced pulmonary toxicity in female albino Wistar rats. The animals were treated with sodium fluoride (NaF, 300 ppm in drinking water ad libitum) alone or in combination with PCAME (25 or 50 mg/kg bw/day by oral intubation) for 60 days and analyzed for changes in lung histology, oxidative stress, inflammation, apoptosis and fibrosis markers. PCAME supplementation prevented the F- induced changes in the above markers. Also, altered serum and bronchoalveolar lavage fluid markers and lung histoarchitecture were also restored by PCAME. F- induced modulations in oxidative stress markers, TUNEL positive cells and mRNA levels of inflammatory genes further normalized by PCAME in lung tissues. These results revealed that PCAME effectively attenuated the F- induced changes in the rat lungs by reducing cellular F- accumulation and enhancing antioxidants level. Reference: Food Chem Toxicol. 2018 Aug;118:235-244. https://pubmed.ncbi.nlm.nih.gov/29758312/

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMF 20.0 112.24
DMF:PBS (pH 7.2) (1:5) 0.2 0.90
DMSO 50.0 280.60
Ethanol 5.0 28.06

Preparing Stock Solutions

The following data is based on the product molecular weight 178.19 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol: 1. Zhang HZ, Li CY, Wu JQ, Wang RX, Wei P, Liu MH, He MF. Anti-angiogenic activity of para-coumaric acid methyl ester on HUVECs in vitro and zebrafish in vivo. Phytomedicine. 2018 Sep 15;48:10-20. doi: 10.1016/j.phymed.2018.04.056. Epub 2018 May 9. PMID: 30195867. 2. Ameeramja J, Kanagaraj VV, Perumal E. Protocatechuic acid methyl ester modulates fluoride induced pulmonary toxicity in rats. Food Chem Toxicol. 2018 Aug;118:235-244. doi: 10.1016/j.fct.2018.05.031. Epub 2018 May 11. PMID: 29758312.
In vitro protocol: 1. Zhang HZ, Li CY, Wu JQ, Wang RX, Wei P, Liu MH, He MF. Anti-angiogenic activity of para-coumaric acid methyl ester on HUVECs in vitro and zebrafish in vivo. Phytomedicine. 2018 Sep 15;48:10-20. doi: 10.1016/j.phymed.2018.04.056. Epub 2018 May 9. PMID: 30195867.
In vivo protocol: 1. Ameeramja J, Kanagaraj VV, Perumal E. Protocatechuic acid methyl ester modulates fluoride induced pulmonary toxicity in rats. Food Chem Toxicol. 2018 Aug;118:235-244. doi: 10.1016/j.fct.2018.05.031. Epub 2018 May 11. PMID: 29758312.

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1: Ameeramja J, Kanagaraj VV, Perumal E. Protocatechuic acid methyl ester modulates fluoride induced pulmonary toxicity in rats. Food Chem Toxicol. 2018 May 11;118:235-244. doi: 10.1016/j.fct.2018.05.031. [Epub ahead of print] PubMed PMID: 29758312.

2: Ameeramja J, Perumal E. Protocatechuic acid methyl ester ameliorates fluoride toxicity in A549 cells. Food Chem Toxicol. 2017 Nov;109(Pt 2):941-950. doi: 10.1016/j.fct.2016.12.024. Epub 2016 Dec 22. PubMed PMID: 28012895.

3: Gebruers E, Cordero-Maldonado ML, Gray AI, Clements C, Harvey AL, Edrada-Ebel R, de Witte PA, Crawford AD, Esguerra CV. A phenotypic screen in zebrafish identifies a novel small-molecule inducer of ectopic tail formation suggestive of alterations in non-canonical Wnt/PCP signaling. PLoS One. 2013 Dec 11;8(12):e83293. doi: 10.1371/journal.pone.0083293. eCollection 2013. PubMed PMID: 24349481; PubMed Central PMCID: PMC3859651.