o-3M3FBS | CAS#313981-55-4 | m-3M3FBS negative control

o-3M3FBS
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WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 563159

CAS#: 313981-55-4

Description: o-3M3FBS is a negative control for m-3M3FBS, an activator of phospholipase C (PLC).

Chemical Structure

o-3M3FBS

CAS# 313981-55-4

Product data Instruction

Theoretical Analysis

MedKoo Cat#: 563159
Name: o-3M3FBS
CAS#: 313981-55-4
Chemical Formula: C16H16F3NO2S
Exact Mass: 343.09
Molecular Weight: 343.364
Elemental Analysis: C, 55.97; H, 4.70; F, 16.60; N, 4.08; O, 9.32; S, 9.34

Price and Availability

Size	Price	Availability	Quantity
5mg	USD 250
10mg	USD 390
50mg	USD 830
Bulk inquiry Welcome, Customer: Please do not inquire quote if your intended use is for a patient since our products are for research use and for chemical synthesis use, not for human use . For in-stock products, we listed price in the web page. You may inquire prices for which sizes were not listed. If no price is listed, this means the product is not in stock at the moment, which may be available via custom synthesis. For cost-effective reason, minimum order of 1g is requested (typically very expensive). The lead time is usually 2-4 months. Quote of less than 1g will not be provided. MedKoo may not offer quote for below reasons: (1) current available synthetic method appears to be extremely expensive which is obviously not affordable to most customers; (2) Key raw material to make the product is temporally not available; (3) DEA controlled substances; (4) the product is under patent protection in customer’s country.

Synonym: o-3M3FBS; o 3M3FBS; o3M3FBS;

IUPAC/Chemical Name: 2,4,6-Trimethyl-N-[2-(trifluoromethyl)phenyl]benzenesulfonamide

InChi Key: SKJJIFRWCCSXGL-UHFFFAOYSA-N

InChi Code: InChI=1S/C16H16F3NO2S/c1-10-8-11(2)15(12(3)9-10)23(21,22)20-14-7-5-4-6-13(14)16(17,18)19/h4-9,20H,1-3H3

SMILES Code: O=S(C1=C(C)C=C(C)C=C1C)(NC2=CC=CC=C2C(F)(F)F)=O

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >3 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.03.00

More Info:

Product Data:

Product Data

Instruction:

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Biological target:	o-3M3FBS is the negative control of m-3M3FBS. o-3M3FBS inhibits inward and outward currents via mechanisms independent of PLC acting in an antagonistic manner.
In vitro activity:	The active compound 2,4,6-trimethyl-N-(meta-3-trifluoromethyl-phenyl)-benzenesulfonamide (m-3M3FBS) stimulated a transient intracellular calcium concentration ([Ca(2+)](i)) increase in neutrophils. Moreover, m-3M3FBS stimulated the formation of inositol phosphates in U937 cells, indicating that it stimulates PLC activity. This study also observed that m-3M3FBS stimulated PLC activity in vitro. Reference: Mol Pharmacol. 2003 May;63(5):1043-50. https://pubmed.ncbi.nlm.nih.gov/12695532/
In vivo activity:	This study examined the effects of m-3M3FBS and 2, 4, 6-trimethyl-N-(ortho-3-trifluoromethyl-phenyl)-benzenesulfonamide (o-3M3FBS), on murine colonic smooth muscle tissue and cells by performing conventional microelectrode recordings, isometric force measurements and patch clamp experiments. Application of m-3M3FBS decreased spontaneous contractility in murine colonic smooth muscle without affecting the resting membrane potential. Patch clamp studies revealed that delayed rectifier K(+) channels were reversibly inhibited by m-3M3FBS and o-3M3FBS. Reference: Eur J Pharmacol. 2010 Feb 25;628(1-3):187-94. https://pubmed.ncbi.nlm.nih.gov/19931239/

Solubility Data

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMF	30.0	87.37
	DMSO	51.5	149.83
	Ethanol	34.3	100.00
	Ethanol:PBS (pH 7.2) (1:3)	0.3	0.73

Preparing Stock Solutions

The following data is based on the product molecular weight 343.36 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:	1. Chen WC, Chou CT, Liou WC, Liu SI, Lin KL, Lu T, Lu YC, Hsu SS, Tsai JY, Liao WC, Liang WZ, Jan CR. Rise of [Ca²⁺]i and apoptosis induced by M-3M3FBS in SCM1 human gastric cancer cells. Chin J Physiol. 2014 Feb 28;57(1):31-40. doi: 10.4077/CJP.2014.BAB141. PMID: 24621336. 2. Bae YS, Lee TG, Park JC, Hur JH, Kim Y, Heo K, Kwak JY, Suh PG, Ryu SH. Identification of a compound that directly stimulates phospholipase C activity. Mol Pharmacol. 2003 May;63(5):1043-50. doi: 10.1124/mol.63.5.1043. PMID: 12695532. 3. Kim SD, Kim HJ, Shim JW, Lee HY, Lee SK, Kwon S, Jung YS, Baek SH, Park JS, Zabel BA, Bae YS. Phospholipase C activator m-3M3FBS protects against morbidity and mortality associated with sepsis. J Immunol. 2012 Aug 15;189(4):2000-5. doi: 10.4049/jimmunol.1200635. Epub 2012 Jul 13. PMID: 22798676. 4. Dwyer L, Kim HJ, Koh BH, Koh SD. Phospholipase C-independent effects of 3M3FBS in murine colon. Eur J Pharmacol. 2010 Feb 25;628(1-3):187-94. doi: 10.1016/j.ejphar.2009.11.029. Epub 2009 Nov 18. PMID: 19931239; PMCID: PMC3152466.
In vitro protocol:	1. Chen WC, Chou CT, Liou WC, Liu SI, Lin KL, Lu T, Lu YC, Hsu SS, Tsai JY, Liao WC, Liang WZ, Jan CR. Rise of [Ca²⁺]i and apoptosis induced by M-3M3FBS in SCM1 human gastric cancer cells. Chin J Physiol. 2014 Feb 28;57(1):31-40. doi: 10.4077/CJP.2014.BAB141. PMID: 24621336. 2. Bae YS, Lee TG, Park JC, Hur JH, Kim Y, Heo K, Kwak JY, Suh PG, Ryu SH. Identification of a compound that directly stimulates phospholipase C activity. Mol Pharmacol. 2003 May;63(5):1043-50. doi: 10.1124/mol.63.5.1043. PMID: 12695532.
In vivo protocol:	1. Kim SD, Kim HJ, Shim JW, Lee HY, Lee SK, Kwon S, Jung YS, Baek SH, Park JS, Zabel BA, Bae YS. Phospholipase C activator m-3M3FBS protects against morbidity and mortality associated with sepsis. J Immunol. 2012 Aug 15;189(4):2000-5. doi: 10.4049/jimmunol.1200635. Epub 2012 Jul 13. PMID: 22798676. 2. Dwyer L, Kim HJ, Koh BH, Koh SD. Phospholipase C-independent effects of 3M3FBS in murine colon. Eur J Pharmacol. 2010 Feb 25;628(1-3):187-94. doi: 10.1016/j.ejphar.2009.11.029. Epub 2009 Nov 18. PMID: 19931239; PMCID: PMC3152466.

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1: Szebenyi SA, Ogura T, Sathyanesan A, AlMatrouk AK, Chang J, Lin W. Increases in intracellular calcium via activation of potentially multiple phospholipase C isozymes in mouse olfactory neurons. Front Cell Neurosci. 2014 Oct 21;8:336. doi: 10.3389/fncel.2014.00336. eCollection 2014. PubMed PMID: 25374507; PubMed Central PMCID: PMC4204526.

2: Dwyer L, Kim HJ, Koh BH, Koh SD. Phospholipase C-independent effects of 3M3FBS in murine colon. Eur J Pharmacol. 2010 Feb 25;628(1-3):187-94. doi: 10.1016/j.ejphar.2009.11.029. Epub 2009 Nov 18. PubMed PMID: 19931239; PubMed Central PMCID: PMC3152466.

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