OG-L002
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    WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 584450

CAS#: 1357302-64-7

Description: OG-L002 is a potent LSD1 inhibitor that has been found to inhibit herpes simplex virus (HSV) immediate early gene expression and viral yield in vitro and suppresses HSV primary infection in vivo.


Chemical Structure

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OG-L002
CAS# 1357302-64-7

Theoretical Analysis

MedKoo Cat#: 584450
Name: OG-L002
CAS#: 1357302-64-7
Chemical Formula: C15H15NO
Exact Mass: 225.12
Molecular Weight: 225.291
Elemental Analysis: C, 79.97; H, 6.71; N, 6.22; O, 7.10

Price and Availability

Size Price Availability Quantity
5mg USD 310
25mg USD 780
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Synonym: OG-L002; OGL002; OG L002

IUPAC/Chemical Name: 4'-((1R,2S)-2-aminocyclopropyl)biphenyl-3-ol

InChi Key: DSOJSZXQRJGBCW-CABCVRRESA-N

InChi Code: InChI=1S/C15H15NO/c16-15-9-14(15)11-6-4-10(5-7-11)12-2-1-3-13(17)8-12/h1-8,14-15,17H,9,16H2/t14-,15+/m1/s1

SMILES Code: OC1=CC(C2=CC=C([C@@H]3[C@@H](N)C3)C=C2)=CC=C1

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Product Data:
Biological target: OG-L002 is a potent and highly selective LSD1 inhibitor with an IC50 of 0.02 μM.
In vitro activity: As shown in Fig. 2, compound OG-L002, which is highly specific to LSD1 (IC50, ~0.02 µM), potently inhibited the expression of viral IE (ICP27 and ICP4) genes, but not cellular control genes. As shown, OG-L002 inhibited viral IE gene expression in both cells with a significantly reduced IC50 (IC50, ~10 µM in HeLa cells; IC50, ~3 µM in HFF cells) relative to the control MAOI TCP (IC50, ~1 mM). In addition, OG-L002 potently reduced viral yields (~100-fold) in comparison to control DMSO treatment. Reference: mBio. 2013 Feb 5;4(1):e00558-12. https://pubmed.ncbi.nlm.nih.gov/23386436/
In vivo activity: Here, this study analysed the activation by lipopolysaccaride (LPS) of primary splenocytes obtained from two different Ogg1-/- mouse strains. This study found that the induction of TNF-α expression was reduced in splenocytes (in particular macrophages) of both Ogg1-/- strains. Notably, an inhibitor of LSD1, OG-L002, reduced the induction of TNF-α mRNA in splenocytes from wild-type mice to the level observed in splenocytes from Ogg1-/- mice and had no influence in the latter cells. Reference: DNA Repair (Amst). 2017 Oct;58:13-20. https://pubmed.ncbi.nlm.nih.gov/28843610/

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMF 50.0 221.94
DMSO 91.9 407.84
DMSO:PBS (pH 7.2) (1:1) 0.5 2.22
Ethanol 17.2 76.24

Preparing Stock Solutions

The following data is based on the product molecular weight 225.29 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol: 1. Tallmadge RL, Žygelytė E, Van de Walle GR, Kristie TM, Felippe MJB. Effect of a Histone Demethylase Inhibitor on Equine Herpesvirus-1 Activity In Vitro. Front Vet Sci. 2018 Mar 12;5:34. doi: 10.3389/fvets.2018.00034. PMID: 29594155; PMCID: PMC5857540. 2. Liang Y, Quenelle D, Vogel JL, Mascaro C, Ortega A, Kristie TM. A novel selective LSD1/KDM1A inhibitor epigenetically blocks herpes simplex virus lytic replication and reactivation from latency. mBio. 2013 Feb 5;4(1):e00558-12. doi: 10.1128/mBio.00558-12. PMID: 23386436; PMCID: PMC3565832. 3. Seifermann M, Ulges A, Bopp T, Melcea S, Schäfer A, Oka S, Nakabeppu Y, Klungland A, Niehrs C, Epe B. Role of the DNA repair glycosylase OGG1 in the activation of murine splenocytes. DNA Repair (Amst). 2017 Oct;58:13-20. doi: 10.1016/j.dnarep.2017.08.005. Epub 2017 Aug 12. PMID: 28843610.
In vitro protocol: 1. Tallmadge RL, Žygelytė E, Van de Walle GR, Kristie TM, Felippe MJB. Effect of a Histone Demethylase Inhibitor on Equine Herpesvirus-1 Activity In Vitro. Front Vet Sci. 2018 Mar 12;5:34. doi: 10.3389/fvets.2018.00034. PMID: 29594155; PMCID: PMC5857540. 2. Liang Y, Quenelle D, Vogel JL, Mascaro C, Ortega A, Kristie TM. A novel selective LSD1/KDM1A inhibitor epigenetically blocks herpes simplex virus lytic replication and reactivation from latency. mBio. 2013 Feb 5;4(1):e00558-12. doi: 10.1128/mBio.00558-12. PMID: 23386436; PMCID: PMC3565832.
In vivo protocol: 1. Seifermann M, Ulges A, Bopp T, Melcea S, Schäfer A, Oka S, Nakabeppu Y, Klungland A, Niehrs C, Epe B. Role of the DNA repair glycosylase OGG1 in the activation of murine splenocytes. DNA Repair (Amst). 2017 Oct;58:13-20. doi: 10.1016/j.dnarep.2017.08.005. Epub 2017 Aug 12. PMID: 28843610.

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Liang et al (2013) A novel selective LSD1/KDM1A inhibitor epigenetically blocks herpes simplex virus lytic replication and reactivation from latency. MBio. 4 e00558 PMID: 23386436