Acid blue 3

    WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 581236

CAS#: 3536-49-0

Description: Acid blue is also known as Patent Blue V, and is a dark bluish synthetic triphenylmethane dye used as a food coloring. As a food additive, it has E number E131. It is a sodium or calcium salt of [4-(α-(4-diethylaminophenyl)-5-hydroxy- 2,4-disulfophenylmethylidene)-2,5-cyclohexadien-1-ylidene] diethylammonium hydroxide inner salt. The color of the dye is pH-dependent. In aqueous solution, its color will vary from a deep blue in alkaline or weakly acidic medium to a yellow–orange in stronger acidic conditions. It is useful as a pH indicator for the range 0.8–3.0. The structure is also redox-sensitive, changing from a reduced yellow form to an oxidized red form in solution. The reduction potential of around 0.77 volts is similar to that of other triphenylmethane dyes. It is usable as a reversible redox indicator in some analytical methods.

Chemical Structure

Acid blue 3
CAS# 3536-49-0

Theoretical Analysis

MedKoo Cat#: 581236
Name: Acid blue 3
CAS#: 3536-49-0
Chemical Formula: C54H62CaN4O14S4
Exact Mass:
Molecular Weight: 1159.42
Elemental Analysis: C, 55.94; H, 5.39; Ca, 3.46; N, 4.83; O, 19.32; S, 11.06

Price and Availability

Size Price Availability Quantity
5.0g USD 200.0 2 Weeks
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Synonym: Acid blue 3; Blue ZN 3; C.I. 42051; Food Blue 3; NSC 5010; L-Blau 3;

IUPAC/Chemical Name: Bis(hydrogen (4-(4-(diethylamino)-5'-hydroxy-2',4'-disulphonatobenzhydrylidene)cyclohexa-2,5-dien-1-ylidene)diethylammonium), calcium salt


InChi Code: InChI=1S/2C27H32N2O7S2.Ca/c2*1-5-28(6-2)21-13-9-19(10-14-21)27(20-11-15-22(16-12-20)29(7-3)8-4)23-17-24(30)26(38(34,35)36)18-25(23)37(31,32)33;/h2*9-18H,5-8H2,1-4H3,(H2-,30,31,32,33,34,35,36);/q;;+2/p-2

SMILES Code: CC/[N+](CC)=C1C=C/C(C=C/1)=C(C2=CC([O-])=C(S(=O)([O-])=O)C=C2S(=O)([O-])=O)/C3=CC=C(N(CC)CC)C=C3.CC/[N+](CC)=C4C=C/C(C=C/4)=C(C5=CC([O-])=C(S(=O)([O-])=O)C=C5S(=O)([O-])=O)/C6=CC=C(N(CC)CC)C=C6.[Ca+2].[H+].[H+]

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

Preparing Stock Solutions

The following data is based on the product molecular weight 1159.42 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL

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