Acetylcysteine amide

    WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 591061

CAS#: 38520-57-9

Description: Acetylcysteine amide prevents Retinal Degeneration by Up-Regulating Reduced Glutathione Production and Reversing Lipid Peroxidation

Chemical Structure

Acetylcysteine amide
CAS# 38520-57-9

Theoretical Analysis

MedKoo Cat#: 591061
Name: Acetylcysteine amide
CAS#: 38520-57-9
Chemical Formula:
Exact Mass:
Molecular Weight:
Elemental Analysis:

Price and Availability

Size Price Availability Quantity
5.0mg USD 290.0 2 Weeks
25.0mg USD 400.0 2 Weeks
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Synonym: Nac amide; NACA

IUPAC/Chemical Name: Propanamide, 2-(acetylamino)-3-mercapto-, (2R)-


InChi Code: InChI=1S/C5H10N2O2S/c1-3(8)7-4(2-10)5(6)9/h4,10H,2H2,1H3,(H2,6,9)(H,7,8)/t4-/m0/s1

SMILES Code: O=C(N)[C@@H](NC(C)=O)CS

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

Preparing Stock Solutions

The following data is based on the product molecular weight Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

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1: Bhatti J, Nascimento B, Akhtar U, Rhind SG, Tien H, Nathens A, da Luz LT. Systematic Review of Human and Animal Studies Examining the Efficacy and Safety of N-Acetylcysteine (NAC) and N-Acetylcysteine Amide (NACA) in Traumatic Brain Injury: Impact on Neurofunctional Outcome and Biomarkers of Oxidative Stress and Inflammation. Front Neurol. 2018 Jan 15;8:744. doi: 10.3389/fneur.2017.00744. eCollection 2017. PubMed PMID: 29387038; PubMed Central PMCID: PMC5776005.

2: Hara Y, McKeehan N, Dacks PA, Fillit HM. Evaluation of the Neuroprotective Potential of N-Acetylcysteine for Prevention and Treatment of Cognitive Aging and Dementia. J Prev Alzheimers Dis. 2017;4(3):201-206. doi: 10.14283/jpad.2017.22. PubMed PMID: 29182711.

3: Gong X, Duan Y, Zheng J, Wang Y, Wang G, Norgren S, Hei TK. Nephroprotective Effects of N-Acetylcysteine Amide against Contrast-Induced Nephropathy through Upregulating Thioredoxin-1, Inhibiting ASK1/p38MAPK Pathway, and Suppressing Oxidative Stress and Apoptosis in Rats. Oxid Med Cell Longev. 2016;2016:8715185. doi: 10.1155/2016/8715185. Epub 2016 Dec 26. PubMed PMID: 28105252; PubMed Central PMCID: PMC5220479.

4: Jastrzębska J, Frankowska M, Filip M, Atlas D. N-acetylcysteine amide (AD4) reduces cocaine-induced reinstatement. Psychopharmacology (Berl). 2016 Sep;233(18):3437-48. doi: 10.1007/s00213-016-4388-5. Epub 2016 Jul 28. PubMed PMID: 27469021.

5: Khayyat A, Tobwala S, Hart M, Ercal N. N-acetylcysteine amide, a promising antidote for acetaminophen toxicity. Toxicol Lett. 2016 Jan 22;241:133-42. doi: 10.1016/j.toxlet.2015.11.008. Epub 2015 Nov 19. PubMed PMID: 26602168.

6: Günther M, Davidsson J, Plantman S, Norgren S, Mathiesen T, Risling M. Neuroprotective effects of N-acetylcysteine amide on experimental focal penetrating brain injury in rats. J Clin Neurosci. 2015 Sep;22(9):1477-83. doi: 10.1016/j.jocn.2015.03.025. Epub 2015 Jun 19. PubMed PMID: 26100161.

7: Paul M, Hemshekhar M, Thushara RM, Sundaram MS, NaveenKumar SK, Naveen S, Devaraja S, Somyajit K, West R, Basappa, Nayaka SC, Zakai UI, Nagaraju G, Rangappa KS, Kemparaju K, Girish KS. Methotrexate Promotes Platelet Apoptosis via JNK-Mediated Mitochondrial Damage: Alleviation by N-Acetylcysteine and N-Acetylcysteine Amide. PLoS One. 2015 Jun 17;10(6):e0127558. doi: 10.1371/journal.pone.0127558. eCollection 2015. PubMed PMID: 26083398; PubMed Central PMCID: PMC4471342.

8: Tobwala S, Khayyat A, Fan W, Ercal N. Comparative evaluation of N-acetylcysteine and N-acetylcysteineamide in acetaminophen-induced hepatotoxicity in human hepatoma HepaRG cells. Exp Biol Med (Maywood). 2015 Feb;240(2):261-72. doi: 10.1177/1535370214549520. Epub 2014 Sep 21. PubMed PMID: 25245075; PubMed Central PMCID: PMC4935326.

9: Pandya JD, Readnower RD, Patel SP, Yonutas HM, Pauly JR, Goldstein GA, Rabchevsky AG, Sullivan PG. N-acetylcysteine amide confers neuroprotection, improves bioenergetics and behavioral outcome following TBI. Exp Neurol. 2014 Jul;257:106-13. doi: 10.1016/j.expneurol.2014.04.020. Epub 2014 May 1. PubMed PMID: 24792639; PubMed Central PMCID: PMC4086163.

10: Atabek Yigit E, Ercal N. Release of N-acetylcysteine and N-acetylcysteine amide from contact lenses. Eye Contact Lens. 2013 Sep;39(5):335-40. doi: 10.1097/ICL.0b013e3182a2f8bc. PubMed PMID: 23945523.

11: Sunitha K, Hemshekhar M, Thushara RM, Santhosh MS, Yariswamy M, Kemparaju K, Girish KS. N-Acetylcysteine amide: a derivative to fulfill the promises of N-Acetylcysteine. Free Radic Res. 2013 May;47(5):357-67. doi: 10.3109/10715762.2013.781595. Epub 2013 Apr 8. Review. PubMed PMID: 23472882.

12: Chen W, Ercal N, Huynh T, Volkov A, Chusuei CC. Characterizing N-acetylcysteine (NAC) and N-acetylcysteine amide (NACA) binding for lead poisoning treatment. J Colloid Interface Sci. 2012 Apr 1;371(1):144-9. doi: 10.1016/j.jcis.2011.12.052. Epub 2011 Dec 27. PubMed PMID: 22284448; PubMed Central PMCID: PMC3342509.

13: Penugonda S, Ercal N. Comparative evaluation of N-acetylcysteine (NAC) and N-acetylcysteine amide (NACA) on glutamate and lead-induced toxicity in CD-1 mice. Toxicol Lett. 2011 Feb 25;201(1):1-7. doi: 10.1016/j.toxlet.2010.11.013. Epub 2010 Dec 9. PubMed PMID: 21145953; PubMed Central PMCID: PMC3387542.

14: Ferreira LF, Campbell KS, Reid MB. Effectiveness of sulfur-containing antioxidants in delaying skeletal muscle fatigue. Med Sci Sports Exerc. 2011 Jun;43(6):1025-31. doi: 10.1249/MSS.0b013e3182019a78. PubMed PMID: 20980926.

15: Whitaker BD, Knight JW. Effects of N-acetyl-cysteine and N-acetyl-cysteine-amide supplementation on in vitro matured porcine oocytes. Reprod Domest Anim. 2010 Oct;45(5):755-9. doi: 10.1111/j.1439-0531.2009.01344.x. PubMed PMID: 19220795.

16: Whitaker BD, Knight JW. Mechanisms of oxidative stress in porcine oocytes and the role of anti-oxidants. Reprod Fertil Dev. 2008;20(6):694-702. PubMed PMID: 18671917.

17: Wu W, Abraham L, Ogony J, Matthews R, Goldstein G, Ercal N. Effects of N-acetylcysteine amide (NACA), a thiol antioxidant on radiation-induced cytotoxicity in Chinese hamster ovary cells. Life Sci. 2008 May 23;82(21-22):1122-30. doi: 10.1016/j.lfs.2008.03.016. Epub 2008 Apr 3. PubMed PMID: 18448127.

18: Ates B, Abraham L, Ercal N. Antioxidant and free radical scavenging properties of N-acetylcysteine amide (NACA) and comparison with N-acetylcysteine (NAC). Free Radic Res. 2008 Apr;42(4):372-7. doi: 10.1080/10715760801998638. PubMed PMID: 18404536.

19: Wu W, Goldstein G, Adams C, Matthews RH, Ercal N. Separation and quantification of N-acetyl-l-cysteine and N-acetyl-cysteine-amide by HPLC with fluorescence detection. Biomed Chromatogr. 2006 May;20(5):415-22. PubMed PMID: 16167305.

20: Penugonda S, Mare S, Goldstein G, Banks WA, Ercal N. Effects of N-acetylcysteine amide (NACA), a novel thiol antioxidant against glutamate-induced cytotoxicity in neuronal cell line PC12. Brain Res. 2005 Sep 21;1056(2):132-8. PubMed PMID: 16120436.