WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 584383

CAS#: 312-48-1

Description: Edrophonium is a rapid-onset, short-acting cholinesterase inhibitor used in cardiac arrhythmias and in the diagnosis of myasthenia gravis. It has also been used as an antidote to curare principles.

Chemical Structure

CAS# 312-48-1

Theoretical Analysis

MedKoo Cat#: 584383
Name: Edrophonium
CAS#: 312-48-1
Chemical Formula: C10H16NO+
Exact Mass: 166.1226
Molecular Weight: 166.2435
Elemental Analysis: C, 72.25; H, 9.70; N, 8.43; O, 9.62

Price and Availability

This product is not in stock, which may be available by custom synthesis. For cost-effective reason, minimum order is 1g (price is usually high, lead time is 2~3 months, depending on the technical challenge). Quote less than 1g will not be provided. To request quote, please email to sales @medkoo.com or click below button.
Note: Price will be listed if it is available in the future.

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Synonym: Edrophonium; Edrophonum

IUPAC/Chemical Name: Benzenaminium, N-ethyl-3-hydroxy-N,N-dimethyl-


InChi Code: InChI=1S/C10H15NO/c1-4-11(2,3)9-6-5-7-10(12)8-9/h5-8H,4H2,1-3H3/p+1

SMILES Code: C[N+](C)(CC)C1=CC=CC(O)=C1

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

Preparing Stock Solutions

The following data is based on the product molecular weight 166.2435 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL

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9: Chinnadurai RK, Saravanaraman P, Boopathy R. The significance of aryl acylamidase activity of acetylcholinesterase in osteoblast differentiation and mineralization. Mol Cell Biochem. 2018 Mar;440(1-2):199-208. doi: 10.1007/s11010-017-3167-x. Epub 2017 Aug 29. PubMed PMID: 28852920.

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12: Fukatsu M, Murakami T, Ohkawara H, Saito S, Ikeda K, Kadowaki S, Sasaki I, Segawa M, Soeda T, Hoshi A, Takahashi H, Shichishima-Nakamura A, Ogawa K, Sugiura Y, Ohto H, Takeishi Y, Ikezoe T, Ugawa Y. A possible role of low regulatory T cells in anti-acetylcholine receptor antibody positive myasthenia gravis after bone marrow transplantation. BMC Neurol. 2017 May 15;17(1):93. doi: 10.1186/s12883-017-0881-7. PubMed PMID: 28506261; PubMed Central PMCID: PMC5433011.

13: Tsunoda K, Fujimaki Y, Morita Y. Detectable voice change with the edrophonium test in laryngeal myasthenia gravis. J Int Med Res. 2017 Oct;45(5):1466-1469. doi: 10.1177/0300060516685026. Epub 2017 Jan 30. PubMed PMID: 28379106; PubMed Central PMCID: PMC5718711.

14: Faiz MA, Ahsan MF, Ghose A, Rahman MR, Amin R, Hossain M, Tareq MNU, Jalil MA, Kuch U, Theakston RDG, Warrell DA, Harris JB. Bites by the Monocled Cobra, Naja kaouthia, in Chittagong Division, Bangladesh: Epidemiology, Clinical Features of Envenoming and Management of 70 Identified Cases. Am J Trop Med Hyg. 2017 Apr;96(4):876-884. doi: 10.4269/ajtmh.16-0842. Epub 2017 Jan 30. PubMed PMID: 28138054; PubMed Central PMCID: PMC5392636.

15: Hagstrom D, Hirokawa H, Zhang L, Radic Z, Taylor P, Collins ES. Planarian cholinesterase: in vitro characterization of an evolutionarily ancient enzyme to study organophosphorus pesticide toxicity and reactivation. Arch Toxicol. 2017 Aug;91(8):2837-2847. doi: 10.1007/s00204-016-1908-3. Epub 2016 Dec 18. PubMed PMID: 27990564.

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19: Matsumoto S, Murakami N, Koizumi H, Takahashi M, Izumi Y, Kaji R. Edrophonium Challenge Test for Blepharospasm. Front Neurosci. 2016 Jun 6;10:226. doi: 10.3389/fnins.2016.00226. eCollection 2016. PubMed PMID: 27375406; PubMed Central PMCID: PMC4894005.

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