Ferrostatin-1 | FER-1 | CAS#347174-05-4

Ferrostatin-1
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WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 555148

CAS#: 347174-05-4

Description: Ferrostatin-1 (Fer-1) is a lipophilic antioxidant that effectively blocks ferroptosis, a distinct non-apoptotic form of cell death caused by lipid peroxidation. Fer-1 inhibited the ROS/RNS generated under rotenone insult in SH-SY5Y cells. Fourth, we revealed the effective role of Fer-1 in ER stress mediated activation of apoptotic pathway. Finally, we reported that Fer-1 mitigated rotenone-induced α-syn aggregation.

Chemical Structure

Ferrostatin-1

CAS# 347174-05-4

Product data Instruction

Theoretical Analysis

MedKoo Cat#: 555148
Name: Ferrostatin-1
CAS#: 347174-05-4
Chemical Formula: C15H22N2O2
Exact Mass: 262.17
Molecular Weight: 262.353
Elemental Analysis: C, 68.67; H, 8.45; N, 10.68; O, 12.20

Price and Availability

Size	Price	Availability
10mg	USD 150	Ready to ship
25mg	USD 250	Ready to ship
50mg	USD 450	Ready to ship
100mg	USD 750	Ready to ship
200mg	USD 1350	Ready to ship
500mg	USD 2650	Ready to ship
1g	USD 3850	2 Weeks
Bulk inquiry Welcome, Customer: Please do not inquire quote if your intended use is for a patient since our products are for research use and for chemical synthesis use, not for human use . For in-stock products, we listed price in the web page. You may inquire prices for which sizes were not listed. If no price is listed, this means the product is not in stock at the moment, which may be available via custom synthesis. For cost-effective reason, minimum order of 1g is requested (typically very expensive). The lead time is usually 2-4 months. Quote of less than 1g will not be provided. MedKoo may not offer quote for below reasons: (1) current available synthetic method appears to be extremely expensive which is obviously not affordable to most customers; (2) Key raw material to make the product is temporally not available; (3) DEA controlled substances; (4) the product is under patent protection in customer’s country.

Synonym: Ferrostatin-1; Frer-1;

IUPAC/Chemical Name: 3-amino-4-(cyclohexylamino)-benzoic acid, ethyl ester

InChi Key: UJHBVMHOBZBWMX-UHFFFAOYSA-N

InChi Code: UJHBVMHOBZBWMX-UHFFFAOYSA-N

SMILES Code: O=C(OCC)C1=CC=C(NC2CCCCC2)C(N)=C1

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Product Data:

Product Data

Certificate of Analysis:

View CoA: current batch, Lot#A23K09S22

Safety Data Sheet (SDS):

View Safety Data Sheet (SDS)

Instruction:

View handling instruction

Biological target:	Ferrostatin-1, a potent and selective ferroptosis inhibitor, suppresses Erastin-induced ferroptosis in HT-1080 cells (EC50=60 nM).
In vitro activity:	The expression levels of AT1R, IL-6, IL-1β, COX-2, and GFAP in the astrocytes were significantly elevated by stimulation with Ang II and greatly suppressed by the introduction of Ferrostatin-1 in a dose-dependent manner. The promoted ROS level and inhibited GSH level in the astrocytes by the stimulation with Ang II were significantly reversed by Ferrostatin-1. Down-regulated GPx4, Nrf2, and HO-1 in the astrocytes induced by Ang II were extremely up-regulated by the treatment of Ferrostatin-1 in a dose-dependent manner. Reference: Int Immunopharmacol. 2021 Jan;90:107179. https://pubmed.ncbi.nlm.nih.gov/33278745/
In vivo activity:	Consistently, the results of in vivo studies demonstrated that ferrostatin-1 protected against light-induced ferroptosis in rats. And it exerted therapeutic effects by inhibiting neuroinflammation and prevented the effects of light exposure on the structure and function of the retina. Reference: Biochem Biophys Res Commun. 2021 Apr 9;548:27-34. https://pubmed.ncbi.nlm.nih.gov/33631670/

Solubility Data

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	53.3	203.20
	DMF	30.0	114.35
	DMF:PBS (pH 7.2) (1:4)	0.2	0.76
	Ethanol	29.4	112.10

Preparing Stock Solutions

The following data is based on the product molecular weight 262.35 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:	1. Li S, Zhou C, Zhu Y, Chao Z, Sheng Z, Zhang Y, Zhao Y. Ferrostatin-1 alleviates angiotensin II (Ang II)- induced inflammation and ferroptosis in astrocytes. Int Immunopharmacol. 2021 Jan;90:107179. doi: 10.1016/j.intimp.2020.107179. Epub 2020 Dec 2. PMID: 33278745. 2. Anthonymuthu TS, Tyurina YY, Sun WY, Mikulska-Ruminska K, Shrivastava IH, Tyurin VA, Cinemre FB, Dar HH, VanDemark AP, Holman TR, Sadovsky Y, Stockwell BR, He RR, Bahar I, Bayır H, Kagan VE. Resolving the paradox of ferroptotic cell death: Ferrostatin-1 binds to 15LOX/PEBP1 complex, suppresses generation of peroxidized ETE-PE, and protects against ferroptosis. Redox Biol. 2021 Jan;38:101744. doi: 10.1016/j.redox.2020.101744. Epub 2020 Oct 16. PMID: 33126055; PMCID: PMC7596334. 3. Tang W, Guo J, Liu W, Ma J, Xu G. Ferrostatin-1 attenuates ferroptosis and protects the retina against light-induced retinal degeneration. Biochem Biophys Res Commun. 2021 Apr 9;548:27-34. doi: 10.1016/j.bbrc.2021.02.055. Epub 2021 Feb 22. PMID: 33631670. 4. Hu B, Liu Y, Chen X, Zhao J, Han J, Dong H, Zheng Q, Nie G. Ferrostatin-1 protects auditory hair cells from cisplatin-induced ototoxicity in vitro and in vivo. Biochem Biophys Res Commun. 2020 Dec 17;533(4):1442-1448. doi: 10.1016/j.bbrc.2020.10.019. Epub 2020 Oct 24. PMID: 33109343.
In vitro protocol:	1. Li S, Zhou C, Zhu Y, Chao Z, Sheng Z, Zhang Y, Zhao Y. Ferrostatin-1 alleviates angiotensin II (Ang II)- induced inflammation and ferroptosis in astrocytes. Int Immunopharmacol. 2021 Jan;90:107179. doi: 10.1016/j.intimp.2020.107179. Epub 2020 Dec 2. PMID: 33278745. 2. Anthonymuthu TS, Tyurina YY, Sun WY, Mikulska-Ruminska K, Shrivastava IH, Tyurin VA, Cinemre FB, Dar HH, VanDemark AP, Holman TR, Sadovsky Y, Stockwell BR, He RR, Bahar I, Bayır H, Kagan VE. Resolving the paradox of ferroptotic cell death: Ferrostatin-1 binds to 15LOX/PEBP1 complex, suppresses generation of peroxidized ETE-PE, and protects against ferroptosis. Redox Biol. 2021 Jan;38:101744. doi: 10.1016/j.redox.2020.101744. Epub 2020 Oct 16. PMID: 33126055; PMCID: PMC7596334.
In vivo protocol:	1. Tang W, Guo J, Liu W, Ma J, Xu G. Ferrostatin-1 attenuates ferroptosis and protects the retina against light-induced retinal degeneration. Biochem Biophys Res Commun. 2021 Apr 9;548:27-34. doi: 10.1016/j.bbrc.2021.02.055. Epub 2021 Feb 22. PMID: 33631670. 2. Hu B, Liu Y, Chen X, Zhao J, Han J, Dong H, Zheng Q, Nie G. Ferrostatin-1 protects auditory hair cells from cisplatin-induced ototoxicity in vitro and in vivo. Biochem Biophys Res Commun. 2020 Dec 17;533(4):1442-1448. doi: 10.1016/j.bbrc.2020.10.019. Epub 2020 Oct 24. PMID: 33109343.

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1: Horwath MC, Bell-Horwath TR, Lescano V, Krishnan K, Merino EJ, Deepe GS Jr.
Antifungal Activity of the Lipophilic Antioxidant Ferrostatin-1. Chembiochem.
2017 Oct 18;18(20):2069-2078. doi: 10.1002/cbic.201700105. Epub 2017 Sep 1.
PubMed PMID: 28783875.

2: Zilka O, Shah R, Li B, Friedmann Angeli JP, Griesser M, Conrad M, Pratt DA. On
the Mechanism of Cytoprotection by Ferrostatin-1 and Liproxstatin-1 and the Role
of Lipid Peroxidation in Ferroptotic Cell Death. ACS Cent Sci. 2017 Mar
22;3(3):232-243. doi: 10.1021/acscentsci.7b00028. Epub 2017 Mar 7. PubMed PMID:
28386601; PubMed Central PMCID: PMC5364454.

3: Ito K, Eguchi Y, Imagawa Y, Akai S, Mochizuki H, Tsujimoto Y. MPP+ induces
necrostatin-1- and ferrostatin-1-sensitive necrotic death of neuronal SH-SY5Y
cells. Cell Death Discov. 2017 Feb 27;3:17013. doi: 10.1038/cddiscovery.2017.13.
eCollection 2017. PubMed PMID: 28250973; PubMed Central PMCID: PMC5327502.

4: Kabiraj P, Valenzuela CA, Marin JE, Ramirez DA, Mendez L, Hwang MS,
Varela-Ramirez A, Fenelon K, Narayan M, Skouta R. The neuroprotective role of
ferrostatin-1 under rotenone-induced oxidative stress in dopaminergic
neuroblastoma cells. Protein J. 2015 Oct;34(5):349-58. doi:
10.1007/s10930-015-9629-7. PubMed PMID: 26385697.

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